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1

Serum TNF-alpha levels in the neoplasm of patients qualified for surgical treatment

100%
Gendek-Kubiak H., Grzegorczyk J., Gendek E. G., Kowalski M. L., Berner J.
Archivum Immunologiae et Therapiae Experimentalis
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2001
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vol. 49
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issue 2 suppl.
97-102
EN
The aim of the study was to analyze serum tumor necrosis factor alpha (TNF-) levels in different neoplasm types qualified for surgical treatment and to evaluate their possible correlations with circulating immune complexes (CIC), IgG, IgM, and thecomplement (C) compounds: C1 inhibitor (C1i), C3c and C4 levels. Studies were performed in sera from 30 neoplasm patients before surgical treatment and in 10 persons from a control group (CG) with no malignancy. Serum TNF- levels were measured with the Cytogen ELISA kit. Average TNF- levels measured in neoplastic patient groups qualified for surgical treatment were not significantly different from the average TNF- level in the CG group.
2

Plasmids R577 and R785 decrease the resistance of Escherichia coli K12 strain W1485 to bactericidal action of normal serum

100%
Jankowski S., Cisowska A.
Journal of Applied Genetics
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1998
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vol. 39
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issue 3
211-216
EN
The role of plasmids of drug resistance R577 and R785 in the development of susceptibility of Escherichia coli K12 strain W1485 to the bactericidal action of serum was studied. Plasmids R577 and R785 were transfered to cells of strain W1485 by means of conjugation. The susceptibility of cells containing the plasmid to serum was compared to that of cells lacking the plasmid. It was found that plasmids R577 and R758 sensitize bacteria to the action of serum.
3
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Lipid metabolism parameters in patients with Alzheimer disease and their first degree relatives

88%
Czyzewski K., Lalowski M. M., Pfeffer A., Barcikowska M.
Acta Neurobiologiae Experimentalis
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2001
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vol. 61
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issue 1
21-26
EN
Recently, it was suggested that the presence of total cholesterol (TC), age and sex interaction in Alzheimers type dementia (AD) is linked with the apolipoprotein E (APOE) genotype. Our objective was to determine whether the serum lipid profile in AD patients and their first degree non-demented relatives of a certain age (NDR) was dependent on APOE genotype. We included 28 mild to moderate AD and 30 NDR according to DSM-III-R and NINCDS-ADRDA criteria. NDR individuals were investigated in an age group similar to the AD group (brother-sister relationship) and in a group including younger individuals (AD patients-children relationship). Our data indicate significant differences between decreased total cholesterol and low density lipoprotein cholesterol ratio in the group of AD patients versus NDR individuals of similar age, independent of APOE genotype, and an increased total cholesterol and low density lipoprotein cholesterol ratio in a group of AD patients versus their children of the same genotype. There was no significant correlation between triglycerides and high density lipoprotein levels with APOE genotype in any of the tested groups. In conclusion, there was a decreased selected lipid serum profile parameters in AD compared to age matched non demented first degree relatives.
4

Application of two different kinds of sera against the Proteus penneri lipopolysaccharide core region in search of epitopes determining cross-reactions with antibodies

88%
Palusiak A., Dzieciatkowska M., Sidorczyk Z.
Archivum Immunologiae et Therapiae Experimentalis
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2008
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vol. 56
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issue 2
135-140
EN
Introduction: Proteus penneri lipopolysaccharide (LPS) core regions are characterized by a greater structural variability than that observed in other Enterobacteriaceae. This fact and the small amount of published data concerning the serological activity of this part of P. penneri LPS prompted an examination of which fragment might determine cross-reactions with antibodies. To date, such epitopes have been found in the LPS core regions of P. mirabilis and P. vulgaris strains. Materials and Methods: Proteus sp. LPSs were tested with unabsorbed rabbit antisera by enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot, and once again by ELISA or passive immunohemolysis after the absorption of these antisera with selected LPSs. Results: The serological studies of P. penneri 8 LPS demonstrated antibodies in the tested antisera recognizing a common epitope located in the core regions of six of the LPSs, i.e. P. penneri 8, 34, 133, 7, 14, and 15. Additionally, another type of antibody directed against some fragment of P. penneri 13 and the core regions of other LPSs investigated was observed in one antiserum. Conclusions: A distal, trisaccharide fragment of the P. penneri 8 LPS core region is suggested to determine the cross-reactions of the tested antisera with the six P. penneri LPSs.
5

Age-dependent effect of limb immobilization and remobilization on rat bone

88%
Trebacz H., Dmowska M., Baj J.
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issue 3-4
121-127
EN
The effect of unilateral hindlimb immobilization and subsequent free remobilization on bone tissue in rats was examined. Right hindlimb of intensively growing (G), young adult (Y) and adult (A) male rats was immobilized by taping for two weeks. Bone tissue was investigated post mortem in experimental and age-matched control rats, either directly after immobilization (Imm) or after two or four weeks of remobilization (Re2, Re4). Apparent density (dapp) and mineralization (Min) were estimated in femora and pelvis. The mechanical state of bone tissue in femora was evaluated using an ultrasonic method. Additionally, activity of serum alkaline phosphatase, and serum calcium and phosphorus were measured in each group. Min and dapp in Imm bones were changed in G rats, while in Y and A only dapp in Imm femora was affected. Velocity of ultrasound was significantly lower in immobilized femora in each age group, indicating decreased elasticity of bone tissue. The differences between immobilized and control limbs were still significant in Re2 and Re4 groups in G rats. In Y rats the differences between experimental and control bones increased during remobilization. It is concluded that deterioration of bone initiated during two weeks of unloading, last within at least four weeks of free remobilization, despite restoration of normal activity.
6

Selenium requirement during pregnancy and in early infancy

75%
Zachara B. A., Czerwionka-Szaflarska M.
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EN
The paper described the up-to-date knowledge on the essential trace element, selenium, in human nutrition, its concentration in blood during pregnancu and in milk during lactation.Changes in selenium concentration in maternal milk during various stages of lactation and selenium concentration in infant blood as well as the selenium intake by breast- and formula-fed infants is discussed.
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