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1

RNA virus-derived expression vectors

100%
Siek A., Figlerowicz M.
Biotechnologia
|
2003
|
issue 2
120-128
EN
Large-scale production of pure, properly folded and biologically active proteins is a requirement of industry as well as of basic sciences. Although many expression systems have been developed, not all of them entirely comply with the conditions of the synthesis of therapeuticals. The application of plants as bioreactors seems to be a promising solution for transient expression of recombinant genes. The article reviews some strategies used in the construction of RNA virus-derived vectors risen for this purpose.
2

Infectious cDNA clones of plant viruses

100%
Sadowy E., Zagorski W., Hulanicka D.
Biotechnologia
|
1996
|
issue 3
60-68
EN
Obtaining of infectious clones corresponding to the genomes of RNA viruses has greatly enhanced investigations.The different strategies employed to construct infectious clones from plant RNA virus and parameters affecting infectivity of such clones have been described.The system represent a powerful tool fo studying plant virus replication, short and long distance virus movement and the virus - host interactions.It may serve as autonomously replicating vectors for the expression of foreign genes in plants.
3

RNA recombination in the virus-transgenic plant system ? the risk of creating a new virus

100%
Urbanowicz A., Alejska M., Figlerowicz M., Bujarski J. J.
Biotechnologia
|
2003
|
issue 2
129-140
EN
It is generally assumed that RNA recombination is one of the major driving forces in the evolution of plant viruses. This process leads to rearrangements of viral genomes and plays an important role in adaptation, genome repair and genetic variability of RNA viruses. It has been observed that viruses could recombine not only with each other, but also with mRNA of transgenic plants expressing viral genes. This observation has given rise to new concerns about creating virus-resistant transgenic plants, because the recombination could generate the viruses with new properties that were different from the parental strains. In this article, we present the current state of knowledge about recombination between transgens and challenging virus, we discuss what may happen in a field during interaction between the virus and the transgenic plant, and we propose strategies that allow to control the virus-transgene crossovers.
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