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Isoenergetic microarrays, a new method to study the structure of RNA

100%

Kierzek E., Fratczak A.

Biotechnologia

2008

issue 4

144-153

RNA is one of the most important molecule essential for proper functioning of the cell. However, there are a growing number of RNAs for which biological function and structure are not known. Isoenergetic microarrays have been designed and apply as a new method to study the structure and interactions of RNAs. Isoenergetic microarrays are built with short modified RNA probes. The 2'-O-methylated and LNA-modified oligomer probes have been designed in such a way, that the interaction of each probe with its complementary single stranded fragment of target RNA has comparable thermodynamic stability (is isoenergetic). This method was tested and optimized on several native RNAs.

Preparation of 13C- and 15N-labelled RNA for the structural atudy using multi-dimensional, heteronuclear magnetic resonance techniques

80%

Olejniczak M.

Biotechnologia

1996

issue 1

176-182

The article briefly presents ways of RNA isolation from isotopically labelled bacteria and its subsequent degradation to 13C- and 15N-labelled nucleoside 5'-monophosphates then enzymatically converted to NTPs. 13C- and 15N-labelling of RNA is achieved by in vitro transcription with T7 RNA polymerase using isotopically labelled nucleoside 5'-triphosphates as a substrates and DNA template.

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2 Biotechnologia

1 2008

1 1996

1 Fratczak A.

1 Kierzek E.

1 Olejniczak M.

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Isoenergetic microarrays, a new method to study the structure of RNA

Preparation of 13C- and 15N-labelled RNA for the structural atudy using multi-dimensional, heteronuclear magnetic resonance techniques