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1

Prenatal detection of maternal UPD15 in a new case with i(15p) by Timing Replication Test (TRT) and methylation analysis

100%
Constantinou M., Kaluzewski B., Helszer Z., Zajac E., Nowacka J.
Journal of Applied Genetics
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2003
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vol. 44
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issue 2
209-218
EN
DNA replication kinetics of the Prader-Willi/Angelman Critical Region (PWACR) was studied with and without synchronisation in human amniotic cell cultures obtained from 20 cases with normal karyotype and 4 cases with a marker of chromosome 15, respectively. A Timing Replication Test (TRT) was performed by synchronisation of amniotic cell cultures and followed by interphase FISH to analyse and compare the early/late replication patterns in SNRPN and UBE3A genes between the homologues of chromosome 15. Asynchronous replication patterns of the analysed genes were observed in both amniotic cell cultures but the percentage of interphase nuclei presenting with asynchronous replication was significantly increased in the cultures with synchronisation (40-51%), as compared to those without synchronisation (20-23%). The evaluations, performed by means of TRT, showed asynchronous replication patterns on control values: between 39% and 46% of cells in all the cases with inv dup(15). In contrast, the percentage of cells with asynchronous replication in the case with i(15p) was significantly decreased (3-6%), as compared to the control value, and it may be indicated by uniparental disomy of chromosome 15 (UPD15). In addition, those results have been confirmed by molecular evaluation, using the methylation diagnostic test for diagnosis of the Prader-Willi Syndrome.
2

Influence of replication on organization and evolution of bacterial genomes

100%
Kowalczuk M., Mackiewicz D., Mackiewicz P., Polak N., Cebrat S.
Biotechnologia
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2005
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issue 3
75-90
EN
In bacterial chromosomes, strong bias in nucleotide composition has been observed between differently replicated DNA strands (leading and lagging ones), and also in many species between the regions proximal and distal to the origin of replication (ori). This bias is also reflected in composition and distribution of genes along the chromosome. Several phenomena connected with the replication of the chromosome are responsible for such polarization, especially mutational pressure, repair mechanisms and recombinations, and also selection pressure. All these phenomena are not indifferent for gene evolution and their rearrangements which are strictly connected with the organization of bacterial chromosome.
3

Initiation of chromosomes replication of Streptomyces; comparative anatomy of oriC of eubacteria

100%
Zakrzewska-Czerwinska J.
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vol. 49
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issue 1
149-160
4

Determination of replication initiation site

100%
Zakrzewska-Czerwinska J., Mackiewicz P., Zawilak A., Cebrat S.
Biotechnologia
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2005
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issue 3
91-101
EN
Initiation of bacterial chromosome replication is mediated by a single initiator protein ? DnaA which interacts specifically with multiple DnaA boxes located within the origin of replication oriC. We have applied in silico methods: DNA asymmetry, DnaA box distribution and dnaA gene location to identify the putative replication origins in bacterial chromosomes. The three methods identify the same region as a putative origin in more than half of the analyzed chromosomes. The most universal method of putative oriC identification in bacterial chromosomes is DNA asymmetry, although in some cases it is necessary to apply all three methods. Interestingly, most bacterial chromosomes exhibit an overrepresentation of DnaA boxes; they contain at least one cluster of DnaA boxes in the vicinity of the oriC region that is probably involved in controlling replication initiation.
5

CD8+ T cell suppressor factors and the control of infection, replication and transcription of human imunodeficiency virus

75%
Copeland K. F. T.
Archivum Immunologiae et Therapiae Experimentalis
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2001
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vol. 49
|
issue 1
13-18
EN
CD8+ T cells have been shown to produce factors which modulate HIV-1 replication in both T cells and monocytic cells. Examination of the literature reveals that this modulation may occur by the production of -chemokines which block viral entry. However, another CD8+ T cell-derived activity targets the replication of HIV-1 at the level of transcription. CD8+ T cell factors strongly suppress replication and transcription in T cells and T cell lines, and in contrast, the factors enhance both replication and transcription in cells of the monocyte/macrophage lineage. The enhancement of transcription and replication by CD8+ T cell factors is induced by increased production of TNF by the macrophages. The enhancement is sensitive to pertussis toxin, indicating a G protein-coupled pathway. Thus, CD8+ T cells produce factors which mediate effects on transcription and replication of HIV-1 in a cell type-dependent manner. In this review a summary of the effects of chemokines and CD8-derived factors on HIV-1 transcription and replication is presented. The virus-host cell interactions that participate in the persistent replication of HIV in macrophages and the suppression of these functions in T cells require definition.The identification of CD8+ T cell factors which exert these controls on HIV-1 may lead to promising new therapies for HIV infection.
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