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1

Plankton larvae of invertebrates as an indicator of potential capacity for regeneration of bottom filtra-tor communities in the Gulf of Gdansk

100%
Wolska-Pys M.
|
1998
|
vol. 27
|
issue 4
93-103
EN
In autumn 1994 and summer 1995, the occurrence of larvae of bottom invertebrates was investigated in the coastal waters of the Gulf of Gdansk. The determined abundance of larval stages of molluscs and barnacles suggested that in spite of pollution of the environment the biofiltrator communities in the Gulf have a great potential for regeneration and development. Several areas with potentially best conditions for growth, characterised by highest quantities of the larvae, and especially by a high percentage of large larvae just before their transformation into settled forms, were selected. Bio-technical measures to increase the rates of growth and to facilitate processes of self-purification in the water body are proposed.
2

Regeneration efficiency evaluation of selected rapeseed (Brassica napus ssp. oleifera) doubled haploid lines

100%
Turczynowska A., Broda Z.
Biotechnologia
|
2010
|
issue 2
132-138
EN
Regeneration is an important step in a process of obtaining valuable plants under in vitro conditions. The aim of the research was to characterize the regeneration efficiency of 14 rapeseed (Brassica napus ssp. oleifera) doubled haploid lines which were supposed to reveal different tissue response under in vitro conditions. The plant material consisted of cotyledonous and hypocotylous explants placed on MS medium with the addition of 1mg/l and 0,1mg/l BAP. Regeneration efficiency evaluation was calculated on the basis of shoot regenerating explants number in relation to the total number of explants. The examined genotypes were characterized by varied regeneration ability and closely related DH lines with high and low regeneration efficiency were determined.
3

Regeneration of winter oilseed rape and trials of transformation mediated by Agrobacterium tumefaciens

100%
Starzycki M., Starzycka E., Matuszczak M.
Biotechnologia
|
1999
|
issue 1
164-171
EN
The effects of one strain Agrobacterium tumefaciens LBA 4404/pBI 121, conditions of co-cultivation and growth regulators on regeneration were studied and trials of transformation were performed on winter oilseed rape Brassica napus L. cv. Lirajet, Valesca and Bor. Strong shoots regeneration was observed after co-cultivation without acetosyringone. Among antibiotics that were used in the experiments, kanamycin A and geneticin hampered whereas carbenicillin and rifampicin stimulated shoots regeneration. The presence of nptII gene was confirmed by PCR and K1, K2 primers which generate a fragment of 732 bp in length.
4

Topophysis in adventitious shoots regeneration in vitro in chrysanthemum

100%
Zalewska M., Miler N.
Biotechnologia
|
2010
|
issue 2
89-95
EN
Success of breeding programmes using adventitious shoots technique often depends on the regeneration efficiency. The aim of this study was to investigate the influence of topophysical position of explants on the efficiency of adventitious shoots regeneratio . Uniform, single shoots of chrysanthemum 'Satinbleu' propagated in vitro were divided equally into three topophysical zones: distal, central and proximal. Two leaves and two internodes were isolated from each zone. The explants were cultured for 12 weeks on MS medium supplemented with 0.6 mg l-1 BAP and 2.0 mg l-1 IAA. Due to the highest adventitious shoots regeneration capability and the earliest formation of the shoots, the most suitable explants for breeding programmes for chrysanthemum are internodes excised from distal part of in vitro plantlet.
5

Efficiency of transformation of Polish cultivars of pea (Pisum sativum L.) with various regeneration capacity by using hypervirulent Agrobacterium tumefaciens strains

100%
Pniewski T., Kapusta J.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 2
139-147
EN
An Agrobacterium-mediated transformation method of pea has been developed for several edible and fodder cultivars of pea (Pisum sativum L.), characterized previously in their potential for regeneration via organogenesis. The most appropriate explant, which was susceptible to Agrobacterium infection and capable of regenerating transgenic plants, turned out to be a slice of an immature embryo, including the embryo axis and the basal part of a cotyledon. Three hypervirulent strains of A. tumefaciens were tested: AgL0, AgL1 and EHA105. Each carried the binary vector pP35SGIB containing the uid gene, with an intron under control of the 35S promoter, and the bar gene conferring resistance to phosphinotricin. Strain AgL0 was found to be efficient for the majority of cultivars, followed by AgL1 and EHA105. Transformation efficiency varied from 0.7 to 4.1%, depending on cultivar and Agrobacterium strain. The transformation efficiency of particular pea cultivars did not clearly correspond to their regeneration capacity, which ? although indispensable ? was not a critical parameter of successful transformation. The presence of integrated genes in pea genomic DNA was detected by the PCR. T-DNA was stably transmitted to the progeny, as it was confirmed by Southern hybridization. The activity of introduced genes was analysed by the histochemical GUS assay and by painting leaves or by spraying transgenic plants with the herbicide Basta.
6

The migration of bone marrow-derived non-hematopoietic tissue-committed stem cells is regulated in an SDF-1-, HGF-, and LIF-dependent manner

100%
Kucia M., Wojakowski W., Reca R., Machalinski B., Gozdzik J., Majka M., Baran J., Ratajczak J., Ratajczak M. Z.
Archivum Immunologiae et Therapiae Experimentalis
|
2006
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vol. 54
|
issue 2
121-135
EN
Introduction: Recently we identified in bone marrow (BM) by employing chemotactic isolation to SDF-1 gradient combined with real time RT-PCR analysis a mobile population of CXCR4+ BM mononuclear cells that express mRNA for various markers of early tissue-committed stem cells (TCSCs). In this study we evaluated whether TCSCs respond to other moto-morphogens, such as hepatocyte growth factor (HGF) and leukemia inhibitory factor (LIF). Materials and Methods: We again employed chemotactic isolation combined with real-time RT-PCR analysis to assess whether murine and human BM contain TCSCs that respond to HGF and LIF gradients. We also evaluated expressions of HGF and LIF in damaged organs. Results: We noted that the number of TCSCs is highest in BM from young (1- to 2-month-old) mice and decreases in 1-year-old animals. Murine and human TCSCs 1) respond to HGF and LIF gradients in addition to an SDF-1 gradient, 2) reside in populations of BM-derived non-hematopoietic CD45? cells, and 3) are released (mobilized) from BM into the peripheral blood (PB) during tissue injury (e.g. after partial body irradiation). Conclusions: These findings further support our theory of the BM as a ?hideout' for TCSCs and we suggest that their presence in BM tissue should be considered before experimental evidence is interpreted simply as transdifferentiation/plasticity of hematopoietic stem cells. Since we demonstrated that not only SDF-1, but also HGF and LIF are upregulated in damaged tissues, we postulate that CXCR4+ c-Met+ LIF-R+ TCSC could be mobilized from the BM into the PB, from which they are subsequently chemoattracted to damaged organs, where they play a role in tissue repair/regeneration.
7

Liver regeneration ofter partial hepatectomy. Function of the lipid messengers

88%
Chocian G., Zabielski P., Chabowski A.
Postępy Higieny i Medycyny Doświadczalnej
|
2002
|
vol. 56
|
issue 1
49-71
EN
The paper contains current data on the different ways of the liver regeneration. There is still not enough data about control of the regeneration processes in the liver. However nuclear phosphatidylinositols and sphingomyelins have been shown to play a potent role of messengers signaling. The lipid messengers may be potent factors affecting process of liver regeneration after partial hepatectomy.
8

Roses breeding: in vitro culture and genetic engineering

88%
Orlikowska T., Wach I., Kucharska D.
Biotechnologia
|
2000
|
issue 4
40-47
EN
Prequisites for successful transformation are developed protocols on efficient regeneration of adventitious shoots or somatic embryos. The most important works concerning regeneration, the objectives and achievements in rose transformation are discussed.
9

Alfalfa (Medicago sativa) as an object for transformation by Agrobacterium tumefaciens and in vitro regeneration

75%
Lisova O., Legocki A. B., Legocki A. B.
Biotechnologia
|
2002
|
issue 3
74-90
EN
From the economical point of view alfalfa is a very valuable plant. It has very high yield potential, it is rich in protein, vitamins and minerals, so it is prized as very important feed for horses, beef cattle, sheep, goats and other domestic animals. That is why the improving of alfalfa quality by the methods of molecular biotechnology is a very interesting issue. In this review information about the crucial elements of alfalfa transformation and regeneration are gathered together. Among other following elements, genetic background of plant, A. tumefaciens strains, explant types, in vitro culture medias, plant development stages are discussed. It seems that optimalisation of the transformation and regeneration procedure are individual for every plant genotype and A. tumefaciens strain.
10
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Human cord blood cells can differentiate into retinal nerve cells

75%
Koike-Kiriyama N., Adachi Y., Minamino K., Iwasaki M., Nakano K., Koike Y., Yamada H., Mukaide H., Shigematsu A., Mizokami T., Matsumura M., Ikehara S.
|
EN
Retinal degeneration and dystrophy are the major causes of blindness in the developed world. It has been reported that human cord blood cells (HCBCs) can differentiate into neuron-like cells in vitro. We have recently demonstrated that bone marrow cells (BMCs) of both mice and rats can differentiate into retinal nerve cells (RNCs). In the present study, we show the differentiation capacity of HCBCs into RNCs in vivo. We transplanted lineage-negative HCBCs into the subretinal space of severe combined immunodeficiency (SCID) mice. Two weeks after the transplantation, some of the transplanted cells expressed human nestin, human MAP2, human neuron specific enolase (NSE), beta-III tubulin and also rhodopsin. These results indicate that HCBCs can differentiate into RNCs and suggest that our new strategy could be used for the regeneration of retinal nerve cells in degenerative or dystrophic diseases.
11

Obtaining homozygous carrot plants with the aid of androgenesis in anther cultures

75%
Gorecka K., Krzyzanowska D., Kiszczak W., Kowalska U., Gorecki R.
Biotechnologia
|
2008
|
issue 2
142-152
EN
Experiments with anther cultures of 22 carrot cultivars were carried out to study the effect of various factors on the effectiveness of embryogenesis in these cultures. The factors included: the stage of microsporogenesis, genotype, training of donor plants and their growth conditions. A modified B5 medium (Gamborg, et al. 1968) containing 500 mg L-1 glutamine, 100 mg L-1 serine, 0.1 mg L-1 of 2,4D, 0.1 mg L-1 NAA, 100 g L-1 sucrose and 6.5 g L-1 agar were used to induce androgenesis. Regeneration was carried out on MS media and B5 with reduced concentration of sucrose at 20 g L-1 without aminoacids and hormones or with small amount of hormones. Substrates that were a mixture of various components, such as peat, sand, mineral wool and charcoal, were used for adaptation. Ploidy of the obtained plants was determined by cytometry method. Homozygosity of the plants was established using two isoenzymatic systems: PGI ? phosphoglucose isomerase, and AAT ? aspartate aminotransferase. Anatomical studies of embryogenesis during anther cultures were also carried out to confirm the androgenetic origin of embryos. It was found that the uninucleate stage was the most suitable time to stimulate microspores to produce embryos, and that bud length was a good external indicator of the stage of microsporogenesis. The studied cultivars differed in their ability to undergo androgenesis in vitro. It was shown that it was not necessary to remove all shoots and umbels except the main one. Generally, the embryos were obtained regardless of the way the donor plants were trained, even when the plants were not trained at all. The donor plants grown in a greenhouse produced more embryos than the plants grown in the field. On MS and B5 media without hormones, used to regenerate plants from embryos, secondary embryogenesis was found to take place followed by a conversion of embryos to complete plants, which subsequently resulted in better adaptation (more than 80% of plants became adapted). Cytometric studies revealed that more than 90% of the obtained androgenetic plants had a doubled chromosome complement. By analyzing the AAT and PGI isoenzymes, it was found that the obtained carrot androgenetic plants were homozygotes. Anatomical studies confirmed that embryos were formed from microspores.
12

Participation of jasmonates in differentiation processes in plants

63%
Saniewski M., Urbanek H.
|
2003
|
issue 3
75-86
EN
Jasmonic acid (JA), methyl jasmonate (JA-Me) and their related compounds which are designated as jasmonates, are widely distributed in the plant kingdom and show various important biological activities in the regulation of plant growth and development, resulting in a consideration that they are putative new plant hormones. Endogenous levels of jasmonates, mainly JA, increase rapidly and transiently in plants or their organs under both abiotic and biotic stress conditions. Jasmonates consist of an integral part of the signal transduction chain between stress signal(s) and stress response(s). In this article, we focused on and reviewed the role of jasmonates in control of differentiation processes in tissue cultures, regeneration and micropropagation, somatic embryo formation, tuber initiation and formation. The involvement of jasmonates in tuberization, tuberous root formation and bulb formation was inferred from their ability to induce the processes in vitro, and from changes in the levels of endogenous jasmonates during the growth of the plants which can account for the initiation of tuberization. The tuberization and the expansion of cells induced by jasmonates always involve the reorientation of cortical microtubules. Differential effect of jasmonic acid on cell cycle progression is also presented. It is still an open question about interactions between jasmonates and other hormones (auxin, ethylene, cytokinins, abscisic acid) in the regulation of meristem activities, cell cycle and other physiological processes.
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