Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 6

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  RAPD-PCR
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1
Content available remote

Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer

100%
Biernasiuk A., Korona-Głowniak I., Grzegorczyk A., Malm A.
Acta Biochimica Polonica
|
2014
|
vol. 61
|
issue 4
727-729
EN
Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.
2

Analysis of DNA polymorphism (RAPD-PCR) and reciprocal effects of geese crossbreeds

100%
Lisowski M., Slawinska A., Dluzniewska P., Mazanowski A., Bednarczyk M.
Folia Biologica
|
2008
|
vol. 56
|
issue 3-4
159-164
EN
Commercial geese breeding in Poland is based on two strains ofWhite Italian geese (W11 and 33). The crossbreeds W33 ( paternal line ) and W11 ( maternal line ) are distributed in Poland under the commercial brand of White Ko?uda? goose. However, there are several breeds which are covered by the animal genetic resources conservation program and kept as conservative flocks. These breeds proved invaluable to commercial geese breeding to stabilize body weight, improve muscling and decrease the amount of fat in the carcass of the crossbreeds. Therefore, this study analyzed the reciprocal crossbreeds of White Ko?uda? geese with the individuals from conservative flocks. DNA polymorphism (RAPD-PCR) of the crossbreeds as well as the phenotypic effect of crossbreeding was evaluated. PCR amplification of five RAPD markers resulted in obtaining 14.25 band/crossbreed group. The genetic similarity of the crossbreeds expressed as band sharing frequency (BS) ranged from 0.44 to 0.97. The direction of crossing of theW33 goose with one of the individuals from the conservative flock strongly affected the genetic similarity estimates. The body weight in the 17th or 24th week of life and the percentage of leg muscle weight in the 24th week of life differed significantly depending on the crossbreed genotype. A similar relationship was demonstrated for egg fertilization and number of nestlings per goose. As the lines were differentiated only by origin of the Z chromosome, the background of the differences in genetic polymorphism and the phenotypic records is hypothesized as (i) the linkage of some production traits with sex chromosomes; (ii) the impact of selection on W33 individuals resulting in lower performance of geese with a W33-derived Z chromosome; (iii) genetic imprinting displayed as the effect of eithermaternal or paternal origin of the Z chromosome.
3

Phylogenetic relationships of Paramecium jenningsi strains (classical analysis and RAPD studies)

100%
Przybos E., Skotarczak B., Wodecka B.
Folia Biologica
|
2003
|
vol. 51
|
issue 1-2
85-95
EN
The present studies with application of classical strain crosses and RAPD-PCR analyses showed the existence of different genetic species (syngens) within Paramecium jenningsi. So far the existence of only one syngen has been accepted. It was found that strains from Saudi Arabia, India, and China compose one genetic species (syngen 1) and six strains from Japan compose second genetic species (syngen 2).
4
Content available remote

Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis

88%
Czernicka M., Pławiak J., Muras P.
Acta Biochimica Polonica
|
2014
|
vol. 61
|
issue 2
195-199
EN
Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent - B. nana and 12 hybrids and cluster II grouped the male parent - B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes.
5

Occurrence and ecology of Paramecium jenningsi strains

88%
Przybos E., Fokin S., Stoeck T., Schmidt H.
Folia Biologica
|
1999
|
vol. 47
|
issue 1-2
53-59
EN
The paramecium strain, originating from Japan, Yamaguchi, belongs to Paramecium jenningsi (type of micronuclei, appearance of autogamy, presence of two macronuclear anlagen, dimensions of cells, and the RAPD fingerprints). The finding of Paramecium jenningsi in Japan enlarges the range of the species believed to be limited to a warm or even tropical climate.
6
Publication available in full text mode
Content available

Genotypic differences between isolates of Phytophthora cinnamomi Rands and P. citricola Sawada obtained from twelve nursery plant species

75%
Wiejacha K., Trzewik A., Orlikowski L. B., Szkuta G., Orlikowska T.
|
|
issue 3
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.