Search results

1

Cross-infections with Pseudomonas aeruginosa in patients with cystic fibrosis attending the Warsaw Centre

100%

Milczewska J., Wołkowicz T., Zacharczuk K., Kwiatkowska M., Sands D.

Developmental Period Medicine

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2015

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issue 1

60-65

EN

Aim: 1. To assess the prevalence of cross-infections with P. aeruginosa in order to evaluate the epidemiological situation of this infection in patients with cystic fibrosis attending our centre; 2. To correlate the clinical features of the patients carrying a potentially transmissible strain with the entire study group in order to determine the risk factors and possible effects of its acquisition. Material and methods: 170 Pseudomonas aeruginosa strains obtained from the respiratory tract of 75 cystic fibrosis patients attending the Warsaw Centre in 2011 and 2012 were typed using restriction enzyme analysis-pulsed field gel electrophoresis (Spe I restriction enzyme was used). Simultaneously, the information concerning contacts between patients, as well as several clinical data regarding the course of the disease were collected. Results: Twenty four clusters of strains were detected. The main cluster included 49 isolates derived from 21 patients. The other detected clusters included 2 to 12 isolates derived from 1 to 7 patients. Three clusters comprised the isolates derived from three pairs of siblings. There were 15 clusters containing 2 to 7 strains belonging to the same patient. The remaining 24 patients were infected with their own strains, not fitting any clonal group. Several clinical parameters showed that the 21 patients whose strains constituted the main cluster, were in worse clinical condition than the other patients in the study group. Moreover, the total duration of their hospitalizations in order to perform intravenous antibiotic treatment was longer. Conclusions: 1. Frequent hospitalizations of CF patients with a more severe course of the disease seem to be a risk factor of cross-infections with P. aeruginosa. 2. Intensification of measures to prevent cross-infection, such as hygienic precautions, patient segregation, introduction of home intravenous antibiotic therapy programme, as well as further education of patients and their parents should lead to the improvement of the epidemiological situation in our centre.

PL

Cel: 1. Ocena częstości występowania zakażeń krzyżowych Pseudomonas aeruginosa i ich możliwych konsekwencji klinicznych u pacjentów chorujących na mukowiscydozę leczonych w naszym ośrodku; 2. Porównanie cech klinicznych grupy pacjentów, u których doszło do zakażenia krzyżowego z cechami klinicznymi całej grupy badanej, w celu określenia czynników ryzyka i możliwych skutków takiego zakażenia. Materiał i metody: 170 szczepów Pseudomonas aeruginosa wyizolowanych w latach 2011-2012 z dróg oddechowych 75 pacjentów z mukowiscydozą pozostających pod opieką Instytutu Matki i Dziecka typowano metodą makrogenomowej analizy restrykcyjnej z elektroforezą w zmiennym polu elektrycznym (z wykorzystaniem endonukleazy Spe I). Zbierano wywiady dotyczące najważniejszych parametrów klinicznych, a także kontaktów pomiędzy pacjentami. Wyniki: W toku analizy wyodrębniono 24 grupy klonalne. Największa grupa zawierała 49 izolatów pochodzących od 21 chorych. Inne grupy obejmowały od 2 do 12 izolatów pochodzących od 1 do 7 pacjentów. Trzy grupy zawierały szczepy pochodzące od trzech rodzeństw. Piętnaście grup klonalnych obejmowało od 2 do 7 szczepów należących do tego samego pacjenta. Pozostałych 24 pacjentów było zakażonych szczepami, nie należącymi do żadnej grupy klonalnej. Ocena parametrów klinicznych wykazała, że przebieg choroby u 21 pacjentów, których szczepy stanowiły największą grupę klonalną, był cięższy niż u pozostałych pacjentów w grupie badanej. Także łączny czas trwania ich hospitalizacji (antybiotykoterapii dożylnych) był dłuższy. Wnioski: 1. Częste hospitalizacje pacjentów z mukowiscydozą w gorszym stanie klinicznym wydają się być istotnym czynnikiem ryzyka zakażeń krzyżowych Pseudomonas aeruginosa. 2. Intensyfikacja działań zapobiegających zakażeniom krzyżowym, takich jak środki higieny, izolacja chorych, wprowadzenie w Polsce programu domowej antybiotykoterapii dożylnej, a także dalsza edukacja pacjentów i ich rodziców odnośnie dróg transmisji zakażeń bakteryjnych, prawdopodobnie przyczynią się do poprawy sytuacji epidemiologicznej w naszym ośrodku.

2

Comparison of octenidine dihydrochloride (Octenisept®), polihexanide (Prontosan®) and povidon iodine (Betadine®) for topical antibacterial effects in Pseudomonas aeruginosa-contaminated, full-skin thickness burn wounds in rats

100%

Uygur F., Özyurt M., Evinç R., Hosbul T., Çeliköz B., Haznedaroglu T.

Open Medicine

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2008

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vol. 3

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issue 4

417-421

EN

Pseudomonas aeruginosa is one of the most frequently isolated organisms from infected burn wounds and a significant cause of nosocomial infection and septic mortality among burn patients. In this animal study, three antiseptic agents which were Octenidine dihydrochloride (Octenisept®, Schülke & Mayr, Norderstedt, Germany), polyhexanide (Prontosan®, B. Braun, Melsungen AG, Germany) and povidon iodine (Betadine, Purdue Pharma L.P, Stamford, USA) were compared to assess the antiseptic effect of their applications on experimental burn wounds in in rats contaiminated with P. aeruginosa. All treatment modalities were effective against P. aeruginosa because there were significant differences between treatment groups and control groups. The mean eschar concentrations were not different between polyhexanide and povidon iodine groups, but there were significant differences between the octenidine dihydrochloride group and the other treatment groups, indicating that the Octenidine dihydrochloride significantly eliminated P. aeruginosa more effectively in the tissues compared to the to other agents. All treatment modalities were sufficient to prevent the P. aeruginosa invasion into the muscle and to cause systemic infection. In conclusion, Octenidine dihydrochloride is the most effective antiseptic agent in the treatment of the P. aeruginosa-contaminated burn wounds; Octenidine dihydrochloride can be considered as a treatment choice because of its peculiar ability of limit the frequency of replacing wound dressings.

3

Methods for eradication of the biofilms formed by opportunistic pathogens using novel techniques – A review

100%

Zabielska J., Tyfa A., Kunicka-Styczyńska A.

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

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2016

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vol. 12

26-37

PL

W niekorzystnych warunkach środowiska, mikroorganizmy zasiedlają zarówno powierzchnie abiotyczne, jak i biotyczne takie jak tkanki zwierzęce czy roślinne, tworząc struktury biofilmu charakteryzujące się wysoką opornością. Adhezja mikroorganizmów, szczególnie patogenów oportunistycznych, niesie niebezpieczeństwo zasiedlania materiałów medycznych, co może doprowadzić do infekcji u osób z obniżoną odpornością. Chociaż dotychczasowe badania wskazują różne metody zapobiegania tworzeniu biofilmu, jego całkowita eliminacja ze środowiska jest nadal niemożliwa. Przedstawione opracowanie stanowi przegląd nowoczesnych metod usuwania dojrzałego biofilmu tworzonego przez patogeny oportunistyczne. Spośród wielu metod opisano m.in. zastosowanie: zimnej plazmy, ultradźwięków, pola elektrycznego, ozonowania wody, terapii fagowej, enzymów działających bezpośrednio na macierz biofilmu, bakteriocyn, środków chemicznych syntetycznych oraz pochodzenia naturalnego.

EN

The inconvenient environmental conditions force microorganisms to colonize either abiotic surfaces or animal and plant tissues and, therefore, form more resistant structures – biofilms. The phenomenon of microbial adherence, opportunistic pathogens in particular, is of a great concern. Colonization of medical devices and biofilm formation on their surface, may lead to severe infections mainly in humans with impaired immune system. Although, current research consider various methods for prevention of microbial biofilms formation, still, once a biofilm is formed, its elimination is almost impossible. This study focuses on the overview of novel methods applied for eradication of mature opportunistic pathogens' biofilms. Among various techniques the following: cold plasma, electric field, ultrasounds, ozonated water treatment, phagotherapy, matrix targeting enzymes, bacteriocins, synthetic chemicals and natural origin compounds used for biofilm matrix disruption were briefly described.

4

Złośliwe zapalenie ucha zewnętrznego – opis przypadku

100%

Lepka P., Katkowska A., Barnaś S.

Otorynolaryngologia - przegląd kliniczny

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2018

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vol. 17

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issue 3

118-123

EN

Malignant Otitis Externa is a rare infection in the external auditory canal, involving the temporal bone, that can be life-threatening for the patient. Immunocompromised elderly patients with diabetes are especially prone to the condition. Mortality rate used to be nearly 50%, whereas nowadays, due to long-term antibiotic therapy including fluoroquinolones, third and fourthgeneration cephalosporins, gentamicin and topical therapy, it has been reduced to about 15%. Diagnosis is based on laboratory tests, imaging studies and microbiological testing. The main etiological factor inducing the development of malignant external otitis is Pseudomonas aeruginosa. The paper presents a case report of a 71-year-old man with uncontrolled diabetes and malignant otitis externa. We describe the way an accurate diagnosis was established, together with the process of treatment, which was completed with clinical improvement, both systemic and local. The patient remains under care of the ENT, endocrine and neurological department. This report summarises information about the malignant otitis externa.

PL

„Złośliwe” zapalenie ucha zewnętrznego (malignant external otitis) jest rzadko występującym zakażeniem przewodu słuchowego zewnętrznego i kości podstawy czaszki mogącym zagrażać życiu chorego. Schorzenie to występuje głównie u osób starszych z zaburzeniami odporności oraz cukrzycą. Śmiertelność wynosiła niegdyś około 50%, aktualnie dzięki stosowaniu wła- ściwej, długotrwałej antybiotykoterapii m.in fluorochinolonów, cefalosporyn III i IV generacji, gentamycyny i dzięki leczeniu miejscowemu wynosi około 15%. Rozpoznanie opiera się na badaniach laboratoryjnych, obrazowych i mikrobiologicznych. Czynnikiem etiologicznym izolowanym najczęściej jest Pseudomonas aeruginosa. Przedstawiono przypadek procesu diagnostycznego u 71-letniego pacjenta z niewyrównaną cukrzycą, ze złośliwym zapaleniem ucha zewnętrznego oraz proces terapeutyczny u tego chorego, zakończony poprawą stanu ogólnego i miejscowego. Pacjent pozostaje pod kontrolą w poradniach ambulatoryjnych: laryngologicznej, endokrynologicznej, neurologicznej. Niniejsze opracowanie będzie podsumowaniem wiadomości o złośliwym zapaleniu ucha zewnętrznego.

5

Opportunistic Gram-negative rods' capability of creating biofilm structures on polivynyl chloride and styrene-acronitrile copolymer surfaces

100%

Zabielska J., Kunicka-Styczyńska A., Rajkowska K., Tyfa A.

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issue 4

733-737

EN

Biofilms are highly organized microbial communities displaying high resistance to disinfectants and other external environmental factors. Medical equipment, such as stents and catheters, can be colonized by a variety of bacteria including opportunistic pathogens circulating in the environment and dangerous to immunocompromised patients. Application of materials resistant to biofilm formation will minimize the risk of patients' infection. Hence, the aim of this research was to determine the biofilm growth of environmental bacteria isolates on polyvinyl chloride and styrene-acronitrile copolymer surfaces. Nine strains (Pseudomonas aeruginosa, Burkholderia cepacia and Serratia liquefacies) isolated from cosmetics, and a reference P. aeruginosa strain ATCC 15442, were tested. The ability and dynamics of biofilm formation on intubation catheters (30°C, up to 24 h) in bacterial growth cultures (107-108 CFU/ml) was investigated, with subsequent sonication and quantification by agar plate count method. The results indicated that all the tested bacteria expressed a strong ability for the polymer surface adhesion, reaching 4.6 to 6.7 log CFU/cm2 after 30 minutes. Moreover, for the majority of strains, the level of 24-hour biofilm production was from 6.67-7.61 log CFU/cm2. This research indicates that the environmental strains circulating between the cosmetics and patients may pose a threat of biofilm formation on medical equipment surfaces, and presumably in the clinical surroundings as well.

6

Biosynthesis of cobalt oxide (Co3O4) nanoparticles using plant extract of Camellia sinensis (L.) Kuntze and Apium graveolens L. as the antibacterial application

88%

Urabe A. A., Aziz W. J.

World News of Natural Sciences

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2019

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vol. 24

356-364

EN

In this work, we prepared cobalt oxide nanoparticles using Celery stalks and green tea leaves extract. The synthesized cobalt-oxide NPs were characterized using X-Ray diffraction. This showed the highest peak and top control (222) at (38.18 degree) with regard to Camellia sinensis extract and (220) at (30.14 degree) for Apium graveolens extract, Field Emission scanning electron microscopy (EF_SEM) at the range of 21-72 nm, revealed the highly uniform shape of particles, while UV-Visible spectroscopy techniques recorded the highest value of the absorptive at 230 nm and the energy band gap to be 3.55 eV for Camellia sinensis and 225 nm and energy band gap 3.85 eV at Apium graveolens, respectively. Our results indicate that the best achievable result in inhibiting bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa comes by way of using Camellia sinensis extract (27-29 mm).

7

Contact lens-related combined Pseudomonas aeruginosa, Acanthamoeba and Candida keratitis among adolescents girls

88%

Świerczyńska M., Tronina A., Bogocz M., Mrukwa-Kominek E., Filipek E.

OphthaTherapy

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2022

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vol. 9

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issue 1

36-44

EN

Background: We present a case of combined Pseudomonas aeruginosa, Acanthamoeba and Candida keratitis and clinical course of treatment in two adolescents girls who did not follow the rules of proper use of soft contact lenses. Case reports: Two teenage contact lens users sought medical attention, reporting visual impairment, redness and pain of the eye. Slit lamp examination showed unilateral corneal ulcer. Confocal microscopy revealed images compatible with amoebic cysts and yeasts cells, whereas the results of conjunctival swabs confirmed the presence of Pseudomonas aeruginosa. After topical and general treatment in the first patient, the corneal defect was healed, and visual acuity improved. The girl remains under the constant supervision of an ophthalmological clinic. The medications were gradually reduced, but with time she developed crystalline keratopathy. Regrettably, the second patient, despite the applied treatment had recurrent inflammation and progressive corneal thinning, which required amniotic membrane transplantation to prevent perforation. Conclusion: The early identification of the pathogen as well as adequate treatment is crucial, as contact lens-associated keratitis are potentially sight threatening. Patient and caregiver education, adequate contact lens hygiene and consistent follow-up are important to prevent serious complications.

8

The effect of a combined choline salicylate and cetalkonium chloride gel on particular strains of Pseudomonas aeruginosa, Staphylococcus spp. and Streptococcus spp.

88%

Amangeldykyzy S., Nurlybek A. N., Nurlan A. N., Juszkiewicz K. T., Polski A., Seisembay U. B., Mukasheva A. M., Umirzakovna U. K., Poleszak E.

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2015

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vol. 28

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issue 2

77-80

EN

The ongoing control of virulent bacteria strains is a challenge for today’s medicine. An example of this, is one widely used drug employed in treating less serious external oral and ocular bacterial infections. This is a gel containing both cetalkonium chloride and choline salicylate. However, whether in the era of expanding bacterial resistance this gel is still effective, is not clear. Hence, in our work, its antibacterial effect was studied against 13 strains of Pseudomonas aeruginosa, 6 strains of Staphylococcus spp. and 6 strains of Streptococcus spp. drawn from the collection of the Department of Microbiology, Virology and Immunology, Kazakh National Medical University, as well as against 30 strains of Staphylococcus spp. recently isolated from Kazakh medical students. This work demonstrated that Pseudomonas aeruginosa was insensitive to this preparation in all samples, while the sensitivity of Staphylococcus spp. and Streptococcus spp. was almost halved, compared to untreated samples. An interesting discovery was the greater resistance of strains obtained from student volunteers than from the collection. However, despite the evident resistance of some strains to the combined cetalkonium chloride and choline salicylate gel, we put forward that it can still be used in less serious external bacterial infections.

9

"Ciężkozbrojny" pseudomonas aeruginosa: mechanizmy lekooporności i ich tło genetyczne

88%

Urbanowicz P., Gniadkowski M.

Kosmos

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2017

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vol. 66

|

issue 1

11-29

PL

Występujące powszechnie i narastające zjawisko oporności na antybiotyki wśród bakterii chorobotwórczych jest jednym z największych wyzwań dzisiejszej medycyny zakażeń. Szczególne zagrożenie stanowią zakażenia szpitalne wywoływane przez wielooporne szczepy określonych gatunków, np. Pseudomonas aeruginosa. Jest to patogen oportunistyczny, odznaczający się opornością naturalną na kilka klas stosowanych antybiotyków. Dzięki wysokiej plastyczności genomu, obejmującej różnorodne mutacje funkcjonalne (strukturalne i regulacyjne) oraz pozyskiwanie obcego DNA, jest w stanie szybko adaptować się do niesprzyjających warunków środowiska. Szczególnie niepokoi zdolność nabywania przez P. aeruginosa dodatkowych cech oporności, co w połączeniu z naturalnymi mechanizmami czyni ten patogen wybitnie trudnym do zwalczania. Bakteria ta jest w stanie wywoływać m. in. ostre zapalenie płuc, zakażenia łożyska krwi, skóry i tkanek miękkich (w tym ran operacyjnych i oparzeniowych). Jest również czynnikiem etiologicznym zakażeń przewlekłych, towarzyszących np. mukowiscydozie. Antybiotykami stosowanymi obecnie przeciwko zakażeniom P. aeruginosa są najczęściej cefalosporyny III i IV generacji, karbapenemy, fluorochinolony i aminoglikozydy. W związku z malejącą liczbą dostępnych, skutecznych opcji terapeutycznych pracuje się nad nowymi terapeutykami lub nowatorskim wykorzystywaniem dotąd już poznanych.

EN

The rapid spread of antibiotic resistance (AMR) in pathogenic bacteria is one of the greatest challenges of modern infectiology. In particular, the most threatening are nosocomial infections caused by multi-drug-resistant strains of several major species, such as Pseudomonas aeruginosa. This opportunistic pathogen exibits a broad-spectrum of natural resistance. Due to its high genome plasticity, comprising functional mutations and acquisition of foreign DNA, P. aeruginosa can easily adapt and persist in harsh environmental niches. The critical issue is its outstanding ability to acquire diverse AMR mechanisms, including those encoded by mobile genetic determinants. In addition to the intrinsic resistance, P. aeruginosa can be highly resistant to all of the currently available antipseudomonadal antimicrobials. P. aeruginosa is the etiological agent of a variety of infections, including acute pneumonia, bloodstream infections or skin and soft tissue infections (e. g. postoperative or burn wounds). It is responsible also for chronic infections, like those in cystic fibrosis (CF) patients. The major antimicrobials used in P. aeruginosa infections are newer-generation cephalosporins, carbapenems, fluoroquinolones or aminoglycosides. Owing to limitations of the effective therapeutic options against P. aeruginosa, new antimicrobials and novel indications and thus applications for older drugs are being developed.

10

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

88%

Chabowski K., Junka A. F., Piasecki T., Nowak D., Nitsch K., Smutnicka D., Bartoszewicz M., Moczała M., Szymczyk P.

Metrology and Measurement Systems

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2017

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vol. 24

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issue 2

11

In vitro antibacterial effects of non-thermal atmospheric plasma irradiation on Staphylococcus pseudintermedius and Pseudomonas aeruginosa

75%

Bae S., Lim D., Kim D., Jeon J., Oh T., Bae S., Lim D., Kim D., Jeon J., Oh T.

Polish Journal of Veterinary Sciences

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2020

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vol. 23

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issue 1

12

Three Pseudomonas aeruginosa strains with different protease profiles

75%

Andrejko M., Zdybicka-Barabas A., Janczarek M., Cytryńska M.

|

EN

The proteolytic activity of three Pseudomonas aeruginosa strains, ATCC 27853 - a reference strain, and two clinical isolates was tested. The activity was examined after culturing the bacteria in two different growth media: the minimal M9 medium and rich Luria-Bertani broth (LB). Based on zymograms and protease activity specific assays, it was concluded that the reference strain produced three proteolytic enzymes in the LB medium: protease IV, elastase B and elastase A, while alkaline protease was only produced in the M9 medium. The clinical isolates of P. aeruginosa produced elastase B and alkaline protease when grown in the LB medium and the minimal M9 medium, respectively. PCR analysis confirmed the presence of both the lasB gene encoding elastase B and aprA coding for alkaline protease in the genomes of the three P. aeruginosa strains analyzed. The expression of these genes coding for two important P. aeruginosa virulence factors was dependent on the growth conditions in all the strains studied. The contribution of the extracellular proteinases to the virulence of P. aeruginosa strains used in this study was investigated using an insect model, the greater wax moth Galleria mellonella.

13

Outbreak of Pseudomonas aeruginosa mastitis in a dairy cow herd in northern Greece and its control with an autogenous vaccine

75%

Petridou E. J., Fragkou I. A., Lafi S. Q., Giadinis N. D., Petridou E. J., Fragkou I. A., Lafi S. Q., Giadinis N. D.

Polish Journal of Veterinary Sciences

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2021

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vol. 24

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issue 2

14

Characteristics of Pseudomonas aeruginosa isolates from intensive care unit

75%

Ozer B., Tatman-Otkun M., Memis D., Otkun M.

Open Medicine

|

2009

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vol. 4

|

issue 2

156-163

EN

The study looked at the antimicrobial resistance patterns, serotypes, molecular types, metallo beta-lactamase, and chromosomal betalactamase enzymes of P. aeruginosa strains isolated from the patients and the staffs of the intensive care unit. P. aeruginosa isolates from the patients as nosocomial pathogens and from the staffs were evaluated for their susceptibilities to the antimicrobials by the disk diffusion and E-test methods. Metallo beta-lactamase enzymes were investigated by E-test, the inducibility of β - lactamase enzymes were detected by the disk antagonism test. Serotyping was performed by slide agglutination method. The P. aeruginosa isolates were typed by pulsed field gel electrophoresis. Twenty-five P. aeruginosa strains from the patients and three from the staffs were isolated. Fifteen P. aeruginosa, eleven of which composed of MDR bacteria, were found in serogroup E, 7 strains in G, 4 strains in B, and 1 strain in serogroup A. In all 12 bacteria in the MDR and serogroup E, metallo beta-lactamase enzyme was found to be positive. And in other 15 strains, except the bacterium which could not be serotyped, chromosomal beta-lactamase was found to be positive. The result of the molecular typing showed PFGE A pattern. In conclusion, a pattern in PFGE which included bacteria from MDR and serogroup E, G which was observed in the P. aeruginosa strains which was isolated from the staff’s hands and from the 5 patients, and PFGE F pattern were found to be observed the most. Finally, the two different clonal strains were found to be established in the intensive care.

15

Hemorrhagic pneumonia in neonatal minks in Greece concomitant with Leismania infantum detection

75%

Filioussis G., Petridou E., Papadopoulos D., Karavanis E., Morgan E., Billinis C., Papadopoulos E.

Polish Journal of Veterinary Sciences

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2018

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vol. 21

|

issue 2

Refine search results

Cross-infections with Pseudomonas aeruginosa in patients with cystic fibrosis attending the Warsaw Centre

Comparison of octenidine dihydrochloride (Octenisept®), polihexanide (Prontosan®) and povidon iodine (Betadine®) for topical antibacterial effects in Pseudomonas aeruginosa-contaminated, full-skin thickness burn wounds in rats

Methods for eradication of the biofilms formed by opportunistic pathogens using novel techniques – A review

Złośliwe zapalenie ucha zewnętrznego – opis przypadku

Opportunistic Gram-negative rods' capability of creating biofilm structures on polivynyl chloride and styrene-acronitrile copolymer surfaces

Biosynthesis of cobalt oxide (Co3O4) nanoparticles using plant extract of Camellia sinensis (L.) Kuntze and Apium graveolens L. as the antibacterial application

Contact lens-related combined Pseudomonas aeruginosa, Acanthamoeba and Candida keratitis among adolescents girls

The effect of a combined choline salicylate and cetalkonium chloride gel on particular strains of Pseudomonas aeruginosa, Staphylococcus spp. and Streptococcus spp.

"Ciężkozbrojny" pseudomonas aeruginosa: mechanizmy lekooporności i ich tło genetyczne

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

In vitro antibacterial effects of non-thermal atmospheric plasma irradiation on Staphylococcus pseudintermedius and Pseudomonas aeruginosa

Three Pseudomonas aeruginosa strains with different protease profiles

Outbreak of Pseudomonas aeruginosa mastitis in a dairy cow herd in northern Greece and its control with an autogenous vaccine

Characteristics of Pseudomonas aeruginosa isolates from intensive care unit

Hemorrhagic pneumonia in neonatal minks in Greece concomitant with Leismania infantum detection