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Chemometric analysis of volatiles of propolis from different regions using static headspace GC-MS

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Kaškonienė V., Kaškonas P., Maruška A., Kubilienė L.
Open Chemistry
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2014
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vol. 12
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issue 6
736-746
EN
Six samples of propolis were analyzed in the paper: a sample from Brazil, Estonia, China and three samples from different locations of Uruguay. Static headspace technique coupled with gas chromatography-mass spectrometry analysis has been applied for the determination of the characteristic volatile profile with the aim to differentiate the propolis from different regions. Monoterpenes (α- and β-pinenes) were predominant in all samples, except the sample from China. This sample separated itself by the alcohols 3-methyl-3-buten-1-ol and 3-methyl-2-buten-1-ol, (40.33% and 11.57%, respectively) and ester 4-penten-1-yl acetate (9.04%). α-Pinene and β-pinene composed 64.59–77.56% of volatiles in Brazilian and Uruguayan propolis, and 29.43% in Estonian propolis. Brazilian propolis was distinguished by a high amount of β-methyl crotonaldehyde (10.11%), one of Uruguayan samples 3- by limonene (15.58%), and the Estonian sample - by eucalyptol (25.95%). Statistical investigation of the samples was made applying principal component, hierarchical cluster and K-Means cluster analyses. Various data pre-processing techniques were proposed and used to study and obtain the important volatile compounds contributed to the differentiation of the propolis samples from different regions to separate clusters.
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Total Phenolic, Flavonoid Contents and Antioxidant Activities of Honey and Propolis Collected from the Region of Laghouat (South of Algeria)

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Bakchiche B., Habati M., Benmebarek A., Gherib A.
World News of Natural Sciences
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2017
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vol. 11
91-97
EN
The aim of this study was to determine total phenolic and flavonoid contents and to evaluate the antioxidant activities of two honeys and one propolis sample, collected from the region of Laghouat (South of Algeria). Total phenolic content were determined by using Folin-Ciocalteu reagent as gallic acid equivalent, while flavonoids content was assessed by means of the AlCl3 method as Rutin equivalent. Antioxidant activities of the honeys and propolis were examined by two different methods, namely, scavenging of free radical 2,2-diphenyl-1-picrylhydrazyl and reducing power. The antioxidant activities were compared with standard antioxidants such as Ascorbic acid, BHT and Trolox. The highest level of phenolic was 2385 mg Gallic acid per 100g sample, the highest level of flavonoid was 379 mg Rutin per 100g sample, while the highest protein content was 1177 mg per 100g sample, DPPH (0.026 mg/ml) and TEAC (0.0015) were detected especially in the propolis sample, indicating that it holds good antioxidant properties. A strong positive correlation was found between phenolics, flavonoids, DPPH and TEAC, indicating that in addition to total phenolic content, flavonoid and protein concentrations are good indicators of the antioxidant potential of propolis and honey.
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