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EN
The aim of this study was to investigate the influence of acute stress on salivary fluoride bioavailability. Acute psychological stress was induced using a standardized model of public speaking. The saliva of male subjects was collected before and after the public speaking task and immediately, 10, 30, and 120 minutes after tooth brushing according to the Bass method with a dentifrice containing amine fluoride (AmF) or oral hygiene tablets containing sodium fluoride (NaF). Subjective psychological responses to public speaking were evaluated using the State-Trait Anxiety Inventory (STAI). Saliva cortisol levels were analyzed as an objective stress marker. The saliva secretion rate was measured both before and after public speaking. Saliva fluoride content was measured. Group comparisons were calculated using a univariate analysis of variance (ANOVA). In all analyses, the alpha-level was set at 0.05. Public speaking significantly increased state anxiety (p<0.001) and salivary cortisol concentrations (p<0.001). Acute stress did not influence salivary secretion rates. In the AmF group, stressed individuals exhibited statistically significant (p=0.044) lower fluoride concentration in saliva compared with the nonstressed subjects Acute psychological stress reactions do not cause hyposalivation but decrease fluoride bioavailability after using a dentifrice containing AmF. Fluoride bioavailability during acute stress is dependent on fluoride formulation.
EN
Introduction: Urea phosphate has been used with good results in animal feeding and preservation of wet corn and blood after slaughter that is destined for fodder use. Moreover, that compound, which is also found in poultry manure, shows fungicidal effect. The aim of the present study was to examine disinfecting properties of urea phosphate in mushroom production. Materials and methods: The investigation was carried out using the nutrient Mycobiol C® (containing urea phosphate) to assess the biocidal properties against selected mould fungi and Basidiomycetes fungi as well as its suitability for wood disinfection in mushroom cultivation rooms. Indication was done using the laboratory method on wood artificially infected by selected mould fungi (Ascomycotina and Deuteromycotina) as well as fungi decomposing wood (Basidiomycotina). Moreover, the effect of urea phosphate on the growth of selected bacteria such as Pseudomonas aeruginosa, Pseudomonas tolaasii and Staphylococcus aureus was also investigated. Results and conclusions: The results indicate that urea phosphate shows fungicidal effect against the tested fungi and mycelium Agaricus bisporus, but not Trichoderma viride. The examined compound applied at 7.0 % concentration for 15 minutes showed bactericidal effect against Pseudomonas aeruginosa and Pseudomonas tolaasii.
PL
Wprowadzenie: Fosforan mocznika jest stosowany z dobrymi wynikami w żywieniu zwierząt oraz do konserwowania wilgotnego zboża i krwi poubojowej, przeznaczonych na cele paszowe. Ponadto związek ten obecny w nawozie kurzym wykazuje działanie grzybobójcze. Celem pracy było zbadanie właściwości dezynfekcyjnych fosforanu mocznika w uprawie grzybów. Materiały i metody: Do badań wykorzystano preparat Mycobiol C® (zawierający fosforan mocznika) oceniając jego właściwości biobójcze przeciwko wybranym grzybom pleśniowym i grzybom rodzaju Basidiomycetes, a także jego przydatność do dezynfekcji drewna w pieczarkarniach. Badanie zostało przeprowadzone w warunkach laboratoryjnych na drewnie sztucznie zainfekowanym przez wybrane grzyby pleśniowe (Ascomycotina i Deuteromycotina) jak również grzyby rozkładające drewno (Basidiomycotina). Ponadto zbadano również wpływ fosforanu mocznika na wzrost wybranych bakterii takich jak Pseudomonas aeruginosa, Pseudomonas tolaasii oraz Staphylococcus aureus. Wyniki i wnioski: Uzyskane wyniki wskazują, że fosforan mocznika wywiera działanie grzybobójcze na grzyby patogenne i grzybnię pieczarki Agaricus bisporus, ale nie niszczy Trichoderma viride. Badany związek stosowany w stężeniu 7.0% przez 15 minut działa bakteriobójczo na Pseudomonas aeruginosa i Pseudomonas tolaasii.
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