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G6PD Activity in Malaria Infected Children in Owerri

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Ihenetu F. C., Nwachukwu M. I., Ohalete C. N., Njoku-Obi T. N., Nwachukwu O. I., Okoro C. I., Okereke B. C., Ihenetu S. C.
World News of Natural Sciences
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2017
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vol. 12
63-72
EN
This paper is a study of G6PD activity in malaria-infected children in Owerri. Blood samples were collected from fifty-one (51) children hospitalized in the Federal Medical Center (FMC) and analyzed using standard Medical Laboratory methods. Results revealed that out of the 51 children examined (26 males and 25 females), 39, representing 76.47% of the total malaria infected children numbers, were G6PD deficient, while 12, representing 23.53%, had normal G6PD. Two different plasmodium species and their percentage occurrences were observed. These were: Plasmodium falciparum (78.43%) and Plasmodium malariae (21.57%). The work indicated that male children were more (P < 0.05) deficient than females, with percentage levels of 61.53% and 38.47%, respectively. In addition, children between the ages of 49-60 months was observed to be more (P < 0.05) G6PD deficient (with percentage of 25.64%), while those between 0-12 months were least G6PD deficient (with percentage of 8.3% (P < 0.05)). As being afflicted with malaria carries a high risk of Glucose-6-phosphate dehydrogenase (G6PD) deficiency in children, there is, therefore, urgent need for public enlightenment on public health implications, need for proper hygiene, as well as a need for strategies for preventing and controlling mosquito populations
2
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Bacterially expressed truncated F2 domain of Plasmodium falciparum EBA-140 antigen can bind to human erythrocytes

100%
Rydzak J., Kryńska K., Suchanowska A., Kaczmarek R., Łukasiewicz J., Czerwiński M., Jaśkiewicz E.
Acta Biochimica Polonica
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2012
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vol. 59
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issue 4
685-691
EN
The recently identified erythrocyte binding antigen-140 (EBA-140) is a member of the Plasmodium falciparum DBL family of erythrocyte binding proteins, which are considered as prospective candidates for malaria vaccine development. The EBA-140 ligand is a paralogue of the well-characterized P. falciparum EBA-175 antigen. They share homology of domain structure, including Region II, which consists of two homologous F1 and F2 domains and is responsible for ligand-erythrocyte interaction during invasion. It was shown that the F2 domain of EBA-175 antigen seems to be more important for erythrocyte binding. In order to study activity and immunogenicity of EBA-140 antigen F2 domain, it is necessary to obtain recombinant protein of high purity and in a sufficient amount, which used to pose a challenge due to the high content of disulphide bridges. Here, we present a new method for expression and purification of Plasmodium falciparum EBA-140 antigen F2 domain in E. coli Rosetta-gami strain in fusion with the maltose binding protein (MBP). The truncated F2 domain formed by spontaneous proteolytic degradation of the fusion protein was purified by affinity chromatography on Ni-NTA resin followed by size exclusion chromatography. Molecular mass of this protein was confirmed by mass spectrometry. Its N-terminal amino acid sequencing revealed a proteolytic cleavage site within the F2 domain. The proper folding of the recombinant, truncated F2 domain of EBA-140 antigen was confirmed by circular dichroism analysis. The truncated F2 domain can specifically bind to human erythrocytes but its binding is not as efficient as that of full Region II. This confirms that both the F1 and F2 domains of EBA-140 antigen are required for effective erythrocyte binding.
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Malaria, hepatitis B and their co-infection among pregnant women visiting maternity centers in Akure, Nigeria

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Afolabi O. J., Bakare T. P.
World News of Natural Sciences
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2022
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vol. 45
93-102
EN
The state of pregnancy is considered a high-risk factor for acquisition of malaria and hepatitis B infections due to hormonal changes that occur during pregnancy. The research was aimed at determining the prevalence of malaria, hepatitis B and their co-infection among pregnant women visiting maternity centers in Akure South Local Government. Five hundred pregnant women were examined from Comprehensive Health Centre, Arakale and Mother and Child Hospital, Oke- Aro for malaria and hepatitis B. Thick and thin blood smears were prepared to detect the presence of malaria parasites, hepatitis B virus kit was used to detect hepatitis B virus antigen. The results showed that 65.6% of the pregnant women were infected with malaria parasites, while 3.8% were infected with hepatitis B virus. It was further observed that 2.2% pregnant women who had malaria also had Hepatitis B. Malaria was more prevalent among the age group 21-25years (68.1%), and women in their third trimester (66.7%), while hepatitis B virus was more prevalent among age group 41-45years (14.3%) and women in their second trimester (4.6%). The study therefore revealed high prevalence of malaria and low prevalence of Hepatitis B infections among pregnant women in this study area. Therefore, prompt diagnosis and treatment of the two infections among pregnant women should be intensified to prevent maternal and neonate mortality.
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