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  1. 14 Biotechnologia

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  3. 4 Archivum Immunologiae et Therapiae Experimentalis

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  1. 2 Figlerowicz M.

  2. 2 Gamian A.

  3. 2 Luczak M.

  4. 2 Skiba E.

  5. 2 Skrzydlewska E.

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1

Methodological aspects of proteomic analyses involving two-dimensional electrophoresis and mass spectrometry

100%
Luczak M., Figlerowicz M., Wojtaszek P.
Biotechnologia
|
2009
|
issue 2
7-26
EN
Proteomics is a scientific discipline that focuses on the large-scale study of proteins, particularly their structures, functions and interactions. The proteome is the full complement of proteins expressed by genome. During the last decade, thanks to subset of proteomic techniques and workflows, it has been possible to identify: diagnostic protein biomarkers, potential therapeutic targets and also biotechnologically important plant proteins, like those that give a plant resistance to drought. Over the last years, proteomics has generated a relatively large number of reviews on technical aspects and concepts. Although total automation and reproducibility are possible, the protocols of protein isolation and separation are sample specific. Moreover, most of them address the development of optimal sample preparation protocols of mammalian cells and tissue. In contrast, plant proteomics is still in its infancy, probably because plant material is very recalcitrant. The objective of this review is to pay attention to some methodological aspects of proteomic analyses using 2DE and mass spectrometry, especially in the case of plant material.
2

Interactions of free radicals with proteins

80%
Skrzydlewska E., Farbiszewski R.
|
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vol. 49
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issue 6
7347-766
EN
This review describe the generation of free radicals in the cells under the influence of exogenously and endogenously acting factors.The interactions of free radicals with proteins and amino acids and the consequences of these effects are also presented.
3

Influence of aging on the protein profile of myelin isolated from human brain white matter

80%
Niebroj-Dobosz I., Rafalowska J., Lukasiuk M., Wisniewska W., Dziewulska D.
Neuropatologia Polska
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1992
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vol. 30
|
issue 2
147-154
EN
Myelin proteins composition was examined in material of 20 autoptic cases at ages from 20 to 97 years. The technique of polyacrylamide gel electrophoresis and isotachophoresis was applied. In polyacrylamide gel electrophoresis a progressive increase starting at the age of 60 years of Wolfgram protein at the expense of Folch-Less proteolipid protein and DM-20 protein was observed. The myelin-associated protein started to increase in the 4th and 5th decade of life, returning therafter to values observed in younger cases. The isotachophoretic technique did not differentiate the changes observed in myelin protein in the course of aging.
4

Application of hihg pressure in molecular biology and biotechnology

80%
Krzyzaniak A., Jurczaki J., Barciszewski J.
Biotechnologia
|
1995
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issue 1
148-157
EN
The application of high pressure for biological macromolecules investigations was an object of interest already in the begining of this century.Recently, attention has been paid to this method because of its possible applications in biotechnology.In this paper, we summarized the data existing in the literature on the machanism of high pressure effects on protein and nucleic acids.We also reviewed various practical aplications of high pressure, especially in food industry.
5

Production of therapeutic proteins in the semen of transgenic animals

80%
Lipinski D., Juzwa W., Zeyland J., Slomski R.
Biotechnologia
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2006
|
issue 1
44-52
EN
The expression of the recombinant proteins by transgenic animals represents an opportunity to achieve cost-effective, large-scale production of a wide variety of therapeutics. Among transgenic animal production systems, the transgenic mammary gland is the most advanced. However, the production of proteins in milk is limited by a relatively long interval from birth to first lactation encountered with domestic livestock, the discontinuous nature of the lactation cycle and the substantial time and material investments required to produce transgenic dairy animals. The semen of transgenic boar represents an alternative platform for the production of therapeutic proteins. The expression of such proteins in the male accessory glands, particularly in the seminal vesicle epithelium can be controlled by gene regulatory sequences specific to these tissues. In this review, we consider the possibility of using such regulatory sequences to drive the production of foreign proteins into the seminal fluids of transgenic animals. Application of this technology to pigs which can be ejaculated 2-3 times per week (200-300 ml per ejaculate), could lead to the annual production of several grams of recombinant protein.
6
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Postmicrosomal protein fractions from short-time-predegenerated rat sciatic nerve

80%
Gorka D., Lewin-Kowalik L.-K. J., Lewin-Kowalik J., Larysz-Brysz L.-B. M., Larysz-Brysz M., Golka G. B., Golka B., Swiech-Sabuda S.-S. E., Swiech-Sabuda E., Malecka-Tendera M.-T. E., Malecka-Tendera E.
Acta Neurobiologiae Experimentalis
|
2000
|
vol. 60
|
issue 4
437-445
EN
The postmicrosomal protein fraction obtained from distal stumps of rat sciatic nerves at 0-6 days following transection were investigated by means of one- and two-dimensional electrophoresis. In all experimental groups, total amount of protein was significantly higher than in the control group. Proteins were resolved into 27 bands after SDS-PAGE. Their molecular weights ranged between 16.2 and 335.4 kDa. Eleven fractions displayed significant quantitative differences. After 2-D-electrophoresis, the pI of the proteins ranged from 4.2 to 7.4. They were resolved to 28 molecular masses from 13.5 kDa to 335.4 kDa. The greatest numbers of fractions (90-109) were observed on the 3rd, 4th, 5th and 6th day after nerve transection. Thus, during first 6 days after transection intensive changes in protein fraction content and composition take place in the distal stump of peripheral nerve. These processes seem to be most prominent on the 4th day after lesion. Results confirm our earlier in vivo findings.
7

The oxidative modification of protein during aging

80%
Augustyniak A., Ostrowska J., Skrzydlewska E.
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vol. 55
|
issue 1
53-69
EN
In this review the influence of reactive oxygen species or other reactive substances on the different protein modifications during aging is described. The accumulation of such proteins is observed and many phenomena of aging and age-dependent diseases are due to this accumulation.
8

Methods of measurement of the protein glycation products formed in human organism

80%
Staniszewska M., Gamian A.
Postępy Higieny i Medycyny Doświadczalnej
|
2003
|
vol. 57
|
issue 4
359-368
EN
The advanced glycation end-products (AGEs) are the compounds formed and accumulated in the organism. There is heterogenous group of the glycation products, which have great variety of structural and functional properties. The glycation end-products can be used as markers of the glycation process. Because of the great heterogeneity of the AGEs there is no specific test for their adequate measurement. In this paper there are presented the chromatographic, colorimetric, spectroscopic, mass-spectrometric and serological methods, which are used for determination of AGEs in biological samples. New procedures for the preparation of the model AGEs have been also described. Special attention has been paid to the immunochemical tests. For further assays the model AGE antigens have to be prepared.
9

Some biological properties of outer membrane proteins of Hafnia

80%
Witkowska D., Czarny A., Mulczyk M.
Archivum Immunologiae et Therapiae Experimentalis
|
1992
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vol. 40
|
issue 2
125-128
EN
Outer membrane proteins (OMP) isolated from four antigenically different strains of Hafnia alvei were tested for the toxicity, pyrogenicity, ability to induce Shwartzman reaction as well as for their influence on the leukocyte system. LD50 doses for the studied preparations determined on inbred mice were 18, 20, 28 and 34 mg/kg. These differences in the toxicity of the preparations were reflected in manifestation of Shwartzman reaction; more toxic preparations induced strongest necrohemorrhagic changes at the site of injection. The OMP preparations injected intravenously to rabbits caused moderate increase of body temperature. They induced changes in the number of leukocytes in the animals comparable with those of other preparates of bacterial origin.
10

BH3-only proteins: the lords of death

80%
Fleischer A., Rebollo A., Ayllon V.
Archivum Immunologiae et Therapiae Experimentalis
|
2003
|
vol. 51
|
issue 1
9-17
EN
Although the mechanisms by which Bcl-2 family proteins control the apoptotic machinery of the cell are not fully understood, it becomes clear that the role of BH3-only proteins consists in serving as sensors or sentinels of cellular damage, transducing the apoptotic stimuli to the mitochondria. For this reason, mammalian cells have developed several strategies for their strict regulation throughout evolution. This review aims to highlight the different ways by which BH3-only proteins are controlled, including transcriptional regulation, post-translational modifications and subcellular localization.
11

Identification and genetics of betaglobulin (BgC1) and alphaglobulin (AmiF1) antigenic markers in cattle

80%
Wegrzyn J., Skiba E., Wegrzyn Z.
|
EN
Alloantibodies detecting antigenic determinats called BgC1 and AmiF1 were obtained in cattle.The analysis of determinants' inheritance was done on 1754 offsprings using 72 sires and 1740 dams.The molecule-carriers of both traits were characterized using serological and physico-chemical methods.It was found that identified determinants are markers of beta-globulin and alpha-microglobulin proteins, and of autosomal, dominant genes BgC1 and AmiF1 from independent loci which control them.
12

Bcl-2 proteins in ovarian apoptosis

80%
Opiela J., Katska-Ksiazkiewicz L.
Biotechnologia
|
2006
|
issue 1
90-96
EN
Members of the Bcl-2 family are considered principal players in the cascade of events that activate or inhibit apoptosis. Recent evidence strongly supports fundamental role of Bcl-2 and related proteins in regulating ovarian cell death. This article will provide an overview of the current knowledge regarding Bcl-2 proteins in programmed cell death in development of the ovary and the postnatal ovarian cycles.
13

Thermal hysteresis proteins - structure, fruction, application

80%
Turkiewicz M.
Biotechnologia
|
1999
|
issue 1
11-33
EN
The paper reports on distribution in nature, properties and molecular structure of antifreeze proteins, especially from fish (a continual biosynthesis) and from fish inhabiting northern part of moderate zone waters (a seasonal synthesis). Three main functions of THPs are described, i.e. inhibition of ice crystals growth, inhibition of ice recrystallization, and inhibition of ice nucleating proteins. Main approaches of THPs research, aimed at their commercialization, agricultural application (crop protection, transgenic plants and fish, resitant to low temperatures), food industry (including frozen foods) and medicine (crysoprotection of cells, tissues and organs for transplantions) are discussed. Cryotoxity THPs detected by some researchers is also mentioned.
14

Protein microencapsulation ? methods and application

80%
Piasecka A., Goderska K.
Biotechnologia
|
2010
|
issue 1
34-45
EN
Biomaterials extracted from natural environment may cause several serious problems due to their low stability. More efficient and safe procedures of their stabilization are still demanded to meet the industry requirements. Microencapsulation is one of the possible methods to prolong the action time of active compounds. This paper reports the techniques for preparing microcapsules and their applications with reference to extremely troublesome materials such as native proteins. Special attention is paid to current achievements in microencapsulation and perspectives for the next years, and advantages of this technique. The technology enables reactive or sensitive biomaterials such as enzymes to be turned into stable ingredients. With carefully controlled and fine-tuned release capabilities, microencapsulation is no longer only an added value technique, but the source of totally new systems with matchless properties.
15

Green fluorescent protein (GFP) an ecological transformation marker and a tool for observing processes in the living cell

80%
Sliwinska E.
Biotechnologia
|
2002
|
issue 1
129-135
EN
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been extensively used as a marker of gene expression and protein targeting in intact cells and organisms. Since GFP has no toxic effect on cells and it is easy to detect, it can replace controversial transformation markers such as those for antibiotic or herbicide resistance. The protein can also be used to detect dynamic responses of signal transduction pathways, subcellular localisation of chimeric proteins, and transfection efficiency. It allows visualisation of the organelles and observation of many important cellular processes.
16

Plants as the reactors for production of biopharmaceuticals

80%
Jasinski M., Banasiak J., Frankowska M., Figlerowicz M.
Biotechnologia
|
2006
|
issue 3
53-66
EN
Modern biotechnology is looking for a new source of high quality pharmaceuticals. Interestingly, many of such pharmaceuticals are often natural products provided by plants. An example is a vast array of secondary metabolites. Apart from extraction of these compounds from plant material, many different strategies have been proposed to efficiently produce some of them, using suspension cell cultures. Plants are also a good source of many recombinant proteins, offering a possibility of posttranslational modifications which are limited in prokaryotes. Efficient systems of stable and transient heterologous protein expression for the industrial scale were developed. Here, we briefly introduce such strategies and give examples of different plant products obtained by applying them.
17

An amazing progress in plant transformation methods

80%
Malepszy S.
|
|
issue 4
15-19
EN
A great progress in the higher plants transformation methodology has been observed. It includes the following events: increasing efficiency, development of new markers which are ecologically acceptable, transformation of new monocotyledonous species using A. tumefaciens 'supervectors', control of recombination site.
18

Antigenic markers of protein genes and their nomenclature in cattle

71%
Wegrzyn J., Skiba E.
|
|
issue 3
319-327
EN
The results of previous research on antigenic (allotypic) specificities of immunoglobulins, alpha globulins, beta globulins and lipoproteins in cattle are reviewed. The suitability of heteroantibodies and alloantibodies for identification of this type of markers was analysed. New names/symbols of antigenic markers of proteins in cattle, identified at the Department of Immuno and Cytogenetics of the National Research Institute of Animal Production (Balice/Krak?w, Poland), were introduced.
19

Chemokines, G proteins and natural killer cells

71%
Maghazachi A. A.
Archivum Immunologiae et Therapiae Experimentalis
|
2000
|
vol. 48
|
issue 2
65-72
EN
Natural killer (NK) cells are anti-tumor and anti-viral effector cells. Members of C, CC, CXC and CX3C chemokines induce the chemotaxis and enhance the cytotoxicity of NK cells, suggesting that these cells express receptors for chemokines. The ability of members of chemokines to inhibit the replication of HIV-1 strains, combined with the ability of the same chemokines to activate the anti-viral NK cells, provide compelling evidence for the role of NK cells in eradicating HIV-1 infection. In addition, chemokines induce various intracellular signaling pathways in NK cells, which include activation of the heterotrimeric, and perhaps the small guanine nucleotide binding (G) proteins, as well as the mobilization of intracellular calcium, among other activities. Further, chemokines induce the phosphorylation of chemokine receptors through the recruitment of G protein-coupled receptor kinases (GRKs) resulting in the desensitization and turning off the signals. In this review, I will update the knowledge of the effect of chemokines on NK cell motility and the signal transduction pathways induced by chemokines in these cells.
20

Biochemical properties and clinical effects of the products formed during the glycation of proteins

71%
Staniszewska M., Gamian A.
Postępy Higieny i Medycyny Doświadczalnej
|
2003
|
vol. 57
|
issue 2
123-147
EN
Advanced glycation end-products (AGEs) are formed during non-enzymatic glycation - the process occurring in vitro and in the organism. The glycation products accumulate in tissues and interact with specific receptors, what induces various cellular responses. Some enzymes important in metabolism can be also glycated. The disturbances of homeostasis, related to the glycation products, are the reason for complications observed in diabetes and aging processes. There are presented in this paper: mechanism of the formation of AGEs, their cellular receptor (RAGE), as well as the effects of glycation in aging, diabetes and Alzheimer disease. Finally, there are described the compounds which could be useful as inhibitors of glycation in clinical practice.
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