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1

GMO, conventional and organic crops ? a problem of co-existence

100%
Korbin M.
Biotechnologia
|
2006
|
issue 3
7-15
EN
In the last ten years, the global area of commercially grown, genetically modified (GM) crops has increased more than 50-times to over 90 mln hectares. GM plants are cultivated in twenty one countries (six European). Despite this high adoption rate and future promises, there is still a multitude of concerns about the impact of GM-crops on natural and agricultural environment. In this article, we present: a) current definition of crop co-existence, b) results of the studies on risk assessment and safe co-existence rules, as well as c) examples of wildernesses of public opinion.
2

Investigation of serum cytokine levels and cytokine production in whole blood cultures of paranoid schizophrenic patients

80%
Kaminska T., Wysocka A., Marmurowska-Michalowska H., Dubas-Slemp H., Kandefer-Szerszen M.
Archivum Immunologiae et Therapiae Experimentalis
|
2001
|
vol. 49
|
issue 6
439-446
EN
There is some evidence that the pathophysiology of schizophrenia is related to changes in the innate and adaptive immune systems. In an attempt to define a potential immunological dysfunction in schizophrenia, we measured the serum levels of several cytokines in the sera of 24 patients with paranoid schizophrenia and investigated the cytokine production in whole blood assays after stimulation in vitro with virus (Newcastle disease), phytohemagglutinin (PHA) or bacterial lipopolysaccharide (LPS) and compared them with healthy, normal controls. A significant increase of IL-6, IL-8 and IFN- gamma levels, but a decreased IL-10 level were observed in the sera of patients with schizophrenia. No significant changes in the serum levels of IL-2, IL-4, IFN-alpha and TNF-alpha were detected in these patients. When cytokine production in vitro was examined, a significant defect in PHA-induced IL-2, IL-4 and IFN-gamma, and in virus-induced IFN-alpha production, but no significant alterations in LPS-induced IL-6, IL-10 and TNF-alpha production were observed. In summary, increased serum levels of some cytokines such as IL-6, IL-8 and IFN-gamma indicate an activation of the inflammatory response in schizophrenia, while the in vitro assay indicates significant changes in the Th1 (decreased production of IL-2 and IFN-gamma) and Th2 (decreased production of IL-4) cell system responses. The role of the defective IFN-apha production in the regulation of the imbalance between Th1 and Th2 cell system responses is suggested.
3

Mouse recombinant interleukin 2 ? determining optimal conditions of production and activity evaluation in vitro

80%
Krzyminski P., Dlugonska H.
Biotechnologia
|
2004
|
issue 1
284-290
EN
Interleukin 2 (IL-2) is the fundamental growth factor for T lymphocytes, both in vivo and in vitro. At our department, specific mouse T lymphocyte clones are used for the examination of antigenic structure of Toxoplasma gondii. Their culturing in vitro requires big amounts of IL-2. That is why we determined optimal conditions for producing crude IL-2 preparation, using recombinant myeloma line X63/0 IL-2 as the producer of IL-2 and cytotoxic lymphocyte line CTL-L as the indicator of IL-2 activity.
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