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Resistance of transgenic tobacco lines to Potato Virus Y

100%
Czubacka A., Doroszewska T.
Biotechnologia
|
2010
|
issue 2
72-82
EN
Potato virus Y (PVY) causes a disease which reduces the quality of raw material as well as the yield. This study on resistance included tobacco transgenic lines belonging to four cultivars modified with three genetic constructions, containing Lettuce mosaic virus coat protein gene (LMV CP) and PVY replicase gene used in sense (ROKY1) and antisense (ROKY2) orientation. The plants were inoculated with PVY isolates under greenhouse conditions and tested by DAS-ELISA. The highest effectiveness of antiviral protection was noticed in case of the lines with transgene LMV CP, lower for ROKY2, and the lowest for ROKY1. In following generations, the percentage increase of a number of plants resistant to PVY was observed within the lines containing transgene LMV CP. Cultivar MN 944 showed to be the best genetic background for that transgene, while cultivar AC Gayed for transgene ROKY.
2

Attempts at stimulating tobacco resistance to virus diseases

88%
DoroszewskaT., Chachulska A. M.
Biotechnologia
|
2000
|
issue 4
128-141
EN
Preliminary investigations were carried out on the transformation of high susceptibility tobacco cultivars. The plants were transformed by being infected with Agrobacterium tumefaciens with binary PROK2- derived plasmids carrying a PVY casette in the sense and antisense orientations and with a plasmid carrying the lettuce mosaic virus (LMV) coat protein gene. Kanamycin resistant plants were obtained following transformation. PCR testing of a selected group of regenerants revealed the presence of a transgene. Disease symptoms were absent from 52% of transgenic plants inoculated with PVYN. This was confirmed by ELISA values.In the T1 generation of transgenic plants, 510 (78%) showed no symptoms after inoculation and 155 (22%) showed typical PVYN symptoms. Among 510 symptomless plants 213 (36%) were selected as highly resistant, 207 (31%) as partially resistant and 89 (11%) as tolerant. Forty kanamycin resistant plants of T2 generation were tested with PVYN. Two plants showed vein necrosis, five - vein clearing and 33 plants showed no disease symptoms. ELISA tests allowed to identify 33 plants (84,6%) totally resistant to PVYN. The preliminary data reported here showed more resistant plants in T2 generations.
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