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1

Localization of the calreticulin gene mRNA in unpollinated and pollinated styles of Petunia hybrida

100%
Lenartowska M., Lenartowski R., Bednarska E.
Journal of Applied Genetics
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2001
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vol. 42
|
issue 1
15-20
EN
The objective of our research on Petunia hybrida is to understand the role of calreticulin in the growth of pollen tubes in the pistil. The aim of this study was the first step: finding out whether CRT gene expression takes place in unpollinated and pollinated styles. It was revealed by in situ hybridization that the transcription of the calreticulin gene takes place in the transmitting cells of unpollinated and pollinated styles and in pollen tubes growing in vivo. The mRNA transcripts of the CRT gene were localized mainly on the surface of endoplasmic reticulum (ER) membranes, both in transmitting cells and in the tip cytoplasm of pollen tubes. The results of this study show that calreticulin can be involved in pollen ? pistil interaction in vivo.
2

Evaluation of crossability of Cuphea lanceolata and C.viscosissima based on pollen tube rowth and seed set

86%
Wojciechowski A., Olejniczak J., Adamska E.
Journal of Applied Genetics
|
1996
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vol. 37
|
issue 3
277-384
EN
Many species of Cuphea genus show promise as rich natural sources of valuable seed oils for commercial utility.Among them are C.lancelota and C.viscosissima which seed oils contain mediumm-chain fatty acids.Recoprocal crosses were made between species to assess their usefulnes for Cuphea breeding programme.The degree of crossability between C.lanceolata and C.viscosissima was determinated by pollen grain germinatin and pollen tube growth observed using fluorescence microscopy in addition to capsule formation and seed number per capsule.Treatment of the pollen grain with a laser microbeam reduced the number of germinated pollen grains, tubes growth in the pistil and seed set when C viscisissima plants were used in the crosses as a female form.In the cross C.lanceolata (female form) x C viscosissima the pollen treatment with a laser microbeam improved pollen tube growth but reduced seed set.Crossability evaluated on the basis of pollen tube growth and seed set showed unilateral intraspecific incompatibility in crosses involving C.lanceolata plants as a female form.
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