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issue 4
82-108
EN
Plant secondary products are the substances of great importance in many spheres of human life. In recent years, many methods have been investigated in order to increase yield of these compounds, synthesized in plant in vitro cultures ? systems, which proved to be very useful and efficient for this purpose. Among these techniques, biotic elicitation, although not yet applied to a large scale production, proved to be a very efficient procedure on laboratory scale. One of the major aims of the studies on biotic elicitation of plants is to identify universal and effective, but at the same time the cheapest and simplest elicitors which could be used to increase secondary metabolites' production in plant in vitro cultures. This review focuses on different biotic elicitors of complex composition (e.g.: fungal culture filtrates and homogenates), as well as those with a known structure (e.g.: chitosan or ergosterol). The factors influencing the elicitation process as well as ways of improving efficiency of this method by combining it with other techniques, which can also increase plant tissue productivity, are also discussed.
EN
Plant cell cultures in vitro produce secondary metabolites with varied effectiveness. Despite industrial application of only few cell suspension cultures, considerable progress in research on plant cell biotechnology has been made over the last few years. Transformed organ cultures, especially hairy roots, seem to be an interesting model for stable production of plant metabolites with high yield. In this paper, cultures of hairy roots of Salvia sclarea, S. officinalis, S. miltiorrhiza, S. przewalski, and Centaurium erythracea, as well as transformed shoots and plants of C. erythracea are presented. Also, production of secondary metabolites in these cultures is discussed.
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