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1

PCR-RFLPs within the lactate dehydrogenase (LDH-A) gene of the domestic pigeon (Columba livia var. domestica)

100%
Dybus A., Kmiec M.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 4
501-504
EN
A total of 45 racing pigeons were genotyped using PCR-RFLP method. PCR product of the LDH-A gene was amplified according to the Long-PCR procedure. The amplification products were digested with restriction enzymes. PCR-RFLPs for two restriction enzymes, HaeIII and NlaIV, were observed. Two pairs of alleles LDH-AA and LDH-AB for LDH-A-NlaIV polymorphism and LDH-AC and LDH-AD for LDH-A-HaeIII polymorphism were detected in the homozygous and heterozygous states. Frequencies of alleles were as follows: A ? 0.622, B ? 0.378 and C ? 0.256, D ? 0.744.
2

The role of mono-amine oxidase isozymes on reserpineinduced adrenal hormone release in the pigeon (Columba livia)

88%
Chakrabarti E., Sengupta S., Dasadhikari S., Ghosh A.
Folia Biologica
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1997
|
vol. 45
113-116
EN
The present investigation was performed to study the participation of the specific isozyme of mono-amineoxidase (MAO) during reserpine-modulated release of medullary catecholamines (CAM). This was carried out by pretreatments of pargyline and clorgyline followed by reserpine administration. The findings revealed that the pigeon adrenal contained predominantly MAO-A. Blockade of type B activity by pargyline caused a significant rise in adrenomedullary CAM content in reserpinized pigeons. However, clorgyline (an MAO-A inhibitor) administration followed by reserpine treatment showed no appreciable change in the medullary CAM level, suggesting that type B activity is mainly responsible for reserpine action on the adrenal medulla in the pigeon. In addition, reserpine was also able to decrease the glandular corticosterone content, while pargyline and clorgyline failed to alter this trend in reserpinized pigeons.
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