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Porphyrins and phthalocyanines. Part I. Properties and some applications

100%

Trytek M., Makarska M., Polska K., Radzki S., Fiedurek J.

issue 4

109-127

Nowadays, the chemistry of porphyrins, phthalocyanines and related compounds (usually named as tetrapyrrolic rings) is one of the most interesting and promising subjects of supramolecular chemistry, because of unique photoactive properties of the porphyrin rings and their ability of electron transfer. The possibility of synthesis of tetrapyrroles with countless organic groups substituted to nine reactive centers of porphyrin ring and synthesis of complexes with almost all metal ions and, additionally, easy to modify the molecular structure of such obtained compounds give a chance of using the porphyrins in many different areas of life. In this paper, we present the most significant problems concerning porphyrins and their metal complexes, such as the molecular structures and physicochemical properties. We also show the most interesting applications, including the role of porphyrins as photosensitizers in photolysis of water and in photodynamic therapy (PDT).

In vitro photodynamic therapy with chlorin e6 leads to apoptosis of human vascular smooth muscle cells

88%

Wawrzynska M., Kalas W., Bialy D., Ziolo E., Arkowski J., Mazurek W., Strzadala L.

Archivum Immunologiae et Therapiae Experimentalis

2010

vol. 58

issue 1

67-75

Percutaneous coronary intervention has become the most common and widely implemented method of heart revascularization. However, the development of restenosis remains the major limitation of this method. Photodynamic therapy (PDT) recently emerged as a new and promising method for the prevention of arterial restenosis. Here the efficacy of chlorin e6 in PDT was investigated in vitro using human vascular smooth muscle cells (TG/HA-VSMCs) as one of the cell types crucial in the development of restenosis. PDT-induced cell death was studied on many levels, including annexin V staining, measurement of the generation reactive oxygen species (ROS) and caspase-3 activity, and assessment of changes in mitochondrial membrane potential and fragmentation of DNA. Photosensitization of TG/HA-VSMCs with a 170 M of chlorin e6 and subsequent illumination with the light of a 672-nm diode laser (2 J/cm2) resulted in the generation of ROS, a decrease in cell membrane polarization, caspase-3 activation, as well as DNA fragmentation. Interestingly, the latter two apoptotic events could not be observed in photosensitized and illuminated NIH3T3 fibroblasts, suggesting different outcomes of the model of PDT in various types of cells. The results obtained with human VSMCs show that chlorin e6 may be useful in the PDT of aerial restenosis, but its efficacy still needs to be established in an animal model.

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1 Archivum Immunologiae et Therapiae Experimentalis

1 Biotechnologia

1 2010

1 2005

1 Arkowski J.

1 Bialy D.

1 Fiedurek J.

1 Kalas W.

1 Makarska M.

1 Mazurek W.

1 Polska K.

1 Radzki S.

1 Strzadala L.

1 Trytek M.

1 Wawrzynska M.

1 Ziolo E.

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Porphyrins and phthalocyanines. Part I. Properties and some applications

In vitro photodynamic therapy with chlorin e6 leads to apoptosis of human vascular smooth muscle cells