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1

Phosphorylation of 68 kDa neurofilament proteins has no significant effect on their assembly

100%

Sonska A., Entlicher G.

Acta Neurobiologiae Experimentalis

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1997

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vol. 57

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issue 4

333-338

EN

Native low molecular weight neurofilaments (NF L) from bovine spinal cord with original phosphate content of 0.4 moles of phosphate per 1 mol of protein were phosphorylated with cyclic AMP dependent protein kinase and protein kinase C. In a similar way recombinant mouse NF L proteins which did not contain any phosphate were phosphorylated with the same enzymes in both, the assembled and disassembled forms. The final phosphate content in both types of NF L proteins reached about 4 moles of phosphate per 1 mol of protein. This phosphorylation had no effect on the assembly of NF L into filaments as observed by electron microscopy.

2

Modulation of the composition of AP-1 complex and its impact on transcriptional activity

88%

Kaminska B., Pyrzynska B., Ciechomska I., Wisniewska M.

Acta Neurobiologiae Experimentalis

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2000

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vol. 60

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issue 3

395-402

EN

AP-1 transcription factor known to play a role in cell proliferation and neuronal activation, it is also involved in apoptosis of cells in response to stress, DNA damaging agents or lack of survival signals. AP-1 DNA binding complex is not a single transcritpion factor but a dimer consisting of members of Fos and Jun families. In this review, we discuss evidence that composition of the AP-1 complex is different under various physiological and pathophysiological conditions. Furthermore, we describe biochemical properties of Fos and Jun proteins that may explain the ability of this transcription factor to activate different sets of genes in response to different stimuli. We propose a hypothesis that AP-1 might contribute to distinct biological processes because an activation of specific signaling pathways results in changes of AP-1 composition and/or phosphorylation status and modulates its transactivating potential towards different promoters.

3

Chemical methods of protein isolation from yeasts cells

63%

Stasinska B.

Biotechnologia

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1999

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issue 2

120-130

EN

The paper presents the problem of protein isolation from yeasts cells using alkaline extraction and chemical modification of protein. The influence of these procedures on functional properties, amino acids level, nutritional value and enzyme digestibility is discussed.

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1 Sonska A.

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1 Wisniewska M.

Phosphorylation of 68 kDa neurofilament proteins has no significant effect on their assembly

Modulation of the composition of AP-1 complex and its impact on transcriptional activity

Chemical methods of protein isolation from yeasts cells