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  1. 1 Biotechnologia

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  1. 1 2002

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  1. 1 Jankowski T.

  2. 1 Zielinska-Dawidziak M.

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The separation of biotechnology products in aqueous two-phase systems. Part I ? Theoretical background

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Zielinska-Dawidziak M., Jankowski T.
Biotechnologia
|
2002
|
issue 1
142-152
EN
The article presents the principles of liquid-liquid extraction in aqueous two-phase systems which offer a great potential for the recovery and purification of various biomolecules, cells and organelles from fermentation slurries. These systems consisting of a solution of two polymers (e.g. polyethylene glycol/dextran), polymer and salt (e.g. polyethylene glycol/phosphate) or a thermoseparating polymer (oxyethylene-oxypropylene copolymer) offer mild separation conditions due to the high concentration of water, which makes them suitable for biotechnology applications.
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