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The Influence of Concentration of Acetic Acid and Pepsin Enzyme in Nilem Fish Skin Collagen Extractionto the Amount of Rendement Produced

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Junianto J., Iskandar I., Rizal A., Damayanti W.
World News of Natural Sciences
|
2018
|
vol. 21
164-170
EN
The added value of nilem fish skin needs to be increased. The purpose of this study is to determine the concentration of acetic acid solution combined with the pepsin enzyme in the extraction of collagen from nilem fish skin that is necessary to obtain the highest yield/renderment. The study employed an experimental research method that used a completely randomized factorial design. The first treatment is the concentration of acetic acid solution. This consists of three levels, namely 0.5M, 0.7M and 0.9M. The second treatment is the concentration of the enzyme pepsin. This in turn consists of three levels, namely 0.5 %, 1.0 % and 1.5% (weight / weight). The parameters observed were collagen renderment. The results showed that the combination treatment concentration of 0.7 M solution of acetic acid by the pepsin enzyme at 1.0%, in the extraction of collagen from fish skin, produce the highest yield compared to other combinations. The renderment yield is 6.18%.
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Effect of Using Fermented Lemna sp. in Fish Feed on Growth Rate of Nilem Carp (Osteochilus hasselti)

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Iskandar I., Andriani Y., Rostika R., Zidni I., Riyanti N. A.
World News of Natural Sciences
|
2019
|
vol. 26
157-166
EN
This research aims to determine the highest level of fermented Lemna sp. used in artficial feed to produce optimum growth rate of Nilem Carp. This research was conducted from March to July 2016, in the Ciparanje Laboratory and Aquaculture Laboratory of the Fisheries and Marine Science Faculty, Universitas Padjadjaran. The method used in this research is experimental, and is of a Completely Randomized Design (CRD). It consists of five treatments and three repetitions, providing one commercial diet (as control) and four fermented Lemna sp. levels (10%, 20%, 30%, and 40%). Collected data are analyzed with analysis of variance F-test at 95% confidence level. The parameters observed in this research are divided into four sub-parameters: nutrition changes of fermented Lemna sp., daily growth rate, feed conversion ratio and survival rate. The crude fiber content of Lemna sp. was reduced from 18.37% to 13.57%, and protein content increased from 19.37% to 23.47%, respectively, after fermentation. Based on the results of this research, fermented Lemna sp. used in fish feed at 40% level produced the best results: a daily growth rate at 0.75%, a feed conversion ratio at 3.61 and a survival rate of up to 100%.
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