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Characterization and serological classification of O-specific polysaccharide of Proteus mirabilis TG 276-90 from Proteus serogroup O34

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Kolodziejska K., Siwinska M., Zych K., Rozalski A., Sidorczyk Z.

Archivum Immunologiae et Therapiae Experimentalis

2006

vol. 54

issue 3

223-226

Introduction: Gram-negative bacteria of the genus Proteus from the family Enterobacteriaceae are currently divided into the five species P. mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens and three unnamed Proteus genomospecies 4, 5, and 6. They are important facultative human and animal pathogens which, under favorable conditions, cause mainly intestinal and urinary tract infections, sometimes leading to serious complications such as acute or chronic pyelonephritis and the formation of bladder and kidney stones. In this study we report on the serological properties of the lipopolysaccharide (LPS) of Proteus mirabilis TG 276-90, whose O-polysaccharide chemical structure was described earlier. Materials and Methods: LPS and alkali-treated LPS of a few serologically related Proteus strains and O-antisera against P. mirabilis TG 276-90 and CCUG 4669 (O34) were used. Serological characterization of P. mirabilis TG 276-90 O-specific polysaccharide was done using enzyme immunosorbent assay, passive immunohemolysis test (PIH), inhibition of these tests, SDS/PAGE and Western blot techniques, absorption of rabbit polyclonal O-antisera, and repeated PIH test. Results: Structural and serological investigations showed that the O-polysaccharides of P. mirabilis TG 276-90 and P. vulgaris O34 are identical and that their LPSs differ only in epitopes in the core part. Therefore these two strains could be classified into the same Proteus O34 serogroup. Conclusions: The serological data showed that the beta-D-GalpNAc-(14)-alpha-D-GalpNAc disaccharide is an important epitope of the P. mirabilis TG 276-90 and P. vulgaris O34 LPSs, shared by the P. mirabilis O16 and P. vulgaris TG 251 LPSs. It is responsible for cross-reactions with P. mirabilis TG 276-90 and P. vulgaris O34 O-antisera.

Serological classification and epitope specificity of Proteus vulgaris TG 251 from Proteus serogroup O65

100%

Zych K., Kolodziejska K., Drzewiecka D., Perepelov A. V., Knirel Y. A., Sidorczyk Z.

Archivum Immunologiae et Therapiae Experimentalis

2007

vol. 55

issue 3

187-191

Introduction: Proteus rods are currently subdivided into five named species, i.e. Proteus mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens, and three unnamed Proteus genomospecies 4 to 6. Based on the serospecificity of the lipopolysaccharide (LPS; O-antigen), strains of P. mirabilis and P. vulgaris were divided into 49 O-serogroups and 11 additional O-serogroups were proposed later. About 15 further O-serogroups have been proposed for the third medically important species, P. penneri. Here the serological classification of P. vulgaris strain TG 251, which does not belong to these serogroups, is reported. Serological investigations also allowed characterization of the epitope specificity of its LPS. Materials and Methods: Purified LPSs from five Proteus strains were used as antigens in enzyme immunosorbent assay (EIA), SDS/PAGE, and Western blot and alkali-treated LPSs in the passive immunohemolysis (PIH) test, inhibition of PIH and EIA, and absorption of the rabbit polyclonal O-antisera with the respective LPS. Results: The serological studies of P. vulgaris TG 251 LPS indicated the identity of its O-polysaccharide with that of P. penneri O65. The antibody specificities of P. vulgaris TG 251 and P. penneri O65 O-antisera, were described. Conclusions: P. vulgaris TG 251 was classified to the Proteus O65 serogroup. Two disaccharide-associated epitopes present in P. vulgaris TG 251 and P. penneri O65 LPSs are suggested to be responsible for cross-reactions with three heterologous Proteus strains.

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2007

1 2006

2 Kolodziejska K.

2 Sidorczyk Z.

2 Zych K.

1 Drzewiecka D.

1 Knirel Y. A.

1 Perepelov A. V.

1 Rozalski A.

1 Siwinska M.

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Characterization and serological classification of O-specific polysaccharide of Proteus mirabilis TG 276-90 from Proteus serogroup O34

Serological classification and epitope specificity of Proteus vulgaris TG 251 from Proteus serogroup O65