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Screening of antibiotic-producing bacteria isolated from soil gotten within gaba, Bwari FCT, Abuja

100%
Azode V. U., Anyaegbunam B. C.
World News of Natural Sciences
|
2023
|
vol. 51
61-69
EN
This investigation seeks to determine the antibiotic-producing potential of bacteria in inhibiting the growth of other organisms like Staphylococcus aureus and Escherichia coli. The bacterial isolates were identified using standard cultural, morphological, and biochemical characterization. These were further screened for their antibiotic-producing potential using the Mueller Hinton agar (MHA) media. The identified bacterial isolates had a mean viable count ranging between 1.38×106 / 1,380,000 cfu/ml and 1.52×106 / 1,520,000 cfu/ml. Micrococcus roseus, Brevibacterium species, Bacillus subtilis, Bacillus anthracis, and Bacillus cereus were the predominant bacterial isolates. Nonetheless, the antibiotic test revealed that Bacillus subtilis and Bacillus anthracis showed antimicrobial activity against S. aureus, whereas Bacillus anthracis, Bacillus cereus, and Bacillus subtilis showed zones of inhibition against E. coli. This study shows that Bacillus species have the potential to produce antibiotics and can be used to control microbial growth in the future.
2
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Assessment of Microorganisms in the Rhizosphere Region of Plantain

88%
Teke E. C., Akowei J. S.
World News of Natural Sciences
|
2023
|
vol. 50
178-196
EN
The populations of microorganisms that exist around the roots of plantain in the various farm locations across Toru Orua metropolis of Bayelsa, Nigeria are different due to difference in ecological locations. This paper assessed the different microorganisms in the Rhizosphere region of the sample plantain. Roots samples collected in University of Africa Toru-Orua (UAT) farm, Angalabiri farm and Koroye farm contain high population of bacteria compared to other Area in Toru-orua. In those locations where we have higher population of microorganisms like UAT farm, Angalabirir farm and Koroye farm, there was evidence of good nutrition in those locations. So, microorganisms in essence characteristically enhance the nutrition of plants Nitrobacter winogradkyi was found in roots samples collected in UAT, Angalabiri and Koroye farm due to the presence of cover crops around the plantain. These varieties of fungi were also isolated. The fungal population follows the same trend. They include aspergillus fumigatus, Aspergillus niger and Aspergillus flavus. These are the largest of all microorganisms in the soil. Others were Rhizobium, Nitrobacter, Winogradkyl, Azomona argillis and Pseudomonas aeruginosa. Practices that would enhance nutrition of the plants and the proliferation of bacteria and fungi around the roots of plantain are recommended such as organic matter accumulation in form of green manuring, zero tillage and non-use of chemicals and burning.
3
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Diversity of Microorganisms Associated with Deteriorated Tomatoes Sold by Vendors in Major Markets in Owerri

75%
Dike K. S., Onuoha N.
World News of Natural Sciences
|
2024
|
vol. 54
123-132
EN
Tomatoes hold considerable significance in Nigeria due to their substantial contributions to the employment sector, income generation for cultivators, and satisfaction of domestic and nutritional food requirements. Nonetheless, postharvest deterioration of a considerable quantity of tomato produce occurs in Nigeria as a result of the growth of spoilage organisms. The diversity of microorganisms associated with the deterioration of tomato fruits sold by vendors in four major markets in Owerri was analyzed. One hundred and twenty samples were randomly drawn from the various markets and subjected to standard microbiological techniques Total viable count (2.6 × 104 CFU/g), total coliform count (4.2 × 104 CFU/g), and total fungi count (7.0 × 105 CFU/g) were highest from deteriorated tomato samples analyzed from the relief market. A total of six bacteria genera belonging to Micrococcus sp., Klebsiella sp., Escherichia coli, Proteus sp., Bacillus sp., and Staphylococcus spp. were identified. Staphylococcus spp (31.75%) was the most predominant bacteria isolate associated with the deteriorated tomato samples. This was followed by Escherichia coli 4 (25%), Bacillus sp. 3 (18.75%), Proteus sp. 2 (12.5%), Enterobacter sp. 1 (6.25%), and Micrococcus sp. Penicillium sp., Aspergillus sp., and Rhizopus sp. were also isolated from the deteriorated tomatoes, with Rhizopus sp. being the most predominant isolate 6 (40%) while Aspergillus sp. 4 (26.7%) was the least predominant isolate. The isolation of potential pathogens from deteriorated tomatoes sold by vendors in the four major markets in Owerri represents a significant health risk to consumers. A robust measure to combat post-harvest deterioration of tomatoes should be put in place by government agencies at all levels. Additionally, public enlightenment and awareness campaigns on the consequences of consuming deteriorated tomatoes should be encouraged.
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