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EN
Annona muricata is a recognized medicinal plant species. Despite its usefulness, adequate attention has not been given to its propagation and cultivation. Therefore, this study investigates the effect of some rooting hormones on juvenile stem cuttings of Annona muricata. Uniform, healthy, single node leafy stem cuttings were obtained from eighty (80) uniformly growing seedlings. The cuttings were treated with 1mg/ml of Indole Acetic Acid (IAA), 1 mg/ml of Indole Butyric Acid (IBA), coconut water and distilled water as control, using the quick dip method. The percentage survival of the cuttings, percentage of die back, number of new roots formed per cutting, length of new roots formed, and number of new shoot were assessed after 60 days. Results obtained revealed that the species responded differently to the four treatments. Coconut water had the highest survivor percentage (100%) and IBA had the lowest (25%). Coconut water had the highest mean number of roots (5.25) and IBA had the least (1.75), but there was no significant difference between the values obtained from IBA and distilled water. IAA had the highest mean value (1.80) for new shoots, while IBA had the least value and there was no significant difference between the values obtained from coconut water and distilled water. Significant differences, however, did abound in the root length. Coconut water had the highest mean root length value (4.70 cm), followed by distilled water (1.95 cm), while IBA had 1.55 cm and IAA had the least value (0.80 cm). Thus the use of coconut water might enhance the development of stem cuttings of Annona muricata.
EN
Diabetes mellitus is a chronic disorder which characterized by high concentration of blood glucose. It is known as one of the major deadly diseases that require a serious attention. Many conventional drugs which have been employing in treating the disease are reported to less effective, expensive and some are not locally available. Therefore, in order to search for more effective, inexpensive and locally available drug, this study investigated antidiabetic activity of Cu(II) complex of King of bitters leaves crude extract. The crude extract of the plant was obtained in n-hexane. The metal complex of the crude extract was synthesized and characterized using solubility tests, Infrared and Ultraviolet-Visible spectroscopic analyses. The antidiabetic activities of the crude extract and its metal complex were examined against α-amylase and α-glucosidase enzymes while acarbose drug was employed as a standard drug. The metal complex showed different degrees of solubility in different solvents. Infrared analysis suggested coordination of the crude extract to the metal ion through oxygen donor atom while the formation of the complex was affirmed through the occurrence of d-d transitions in the visible region of the metal complex. The crude extract and its metal complex displayed good activities against α-amylase and α-glucosidase enzymes. It is suggested that the compounds are promising candidates to inhibit α-amylase and α-glucosidase.
EN
Rapid Resolution HPLC/DAD method, on a 1.8 µm, 4.6×50 mm column, was developed to enable a rapid separation of a mixture of 17 compounds, which consisted of hydroxybenzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanone, flavonol-glycoside and antraquinone, in a single run, within 22 minutes. The developed method is precise, accurate and sensitive enough for simultaneous quantitative evaluation of major compounds in crude and hydrolyzed extracts of parsley, buckthorn, mint, caraway and birch. In order to overcome the inability to quantify all the phenolic compounds present in the samples caused by lack of external standards, HPLC approaches for the total phenolic content estimation based on sum of all integrated peak areas were made. These results were compared with the total phenolic content determined by Folin-Cioacalteu method. Although the correlation between the series of data was not significant (pHPLC was not high in the case of parsley, buckthorn and mint extract. Regarding the obtained results, the HPLC approach could serve as an excellent tool for total phenolic content estimation, without the need for complete identification of the individual compounds. [...]
EN
Cissus vitiginea L. is a perennial climber with potent medicinal values and used in Indian traditional systems of medicines to cure wounds, diabetes, cardiovascular illness, cancers, particularly bone diseases and arthritis. This plant is kept under rare category in certain parts of India due to its imprudent harvesting from the wild. Assortment of literature on phytochemical studies of this plant shows the presence of alkaloids, flavonoids, triterpenoids, steroids, glycosides, coumarin, tannins, sugar, proteins etc. The reported secondary metabolites have been reviewed to possess immense biological activities in living systems. In the present review, we have summarized the information concerning the botanical description, phytochemistry, toxicology, pharmacology and the traditional medicinal uses of C. vitiginea. It has been concluded that this plant has not been well explored for its various biological activities and the alternate methods of propagation to increase its natural population in the wild.
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