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Production, purification and characterization of mannanase obtained from Pichia kudriavzevii strain AUMC 10190 isolated from citrus wastes

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Afolabi F. T., Jimoh Y. Z.
World News of Natural Sciences
|
2022
|
vol. 45
1-17
EN
Mannanases from microorganisms are important biotechnologically as they are involved in the hydrolysis of complex polysaccharides of plant tissues into simple molecules. The major mannan-degrading enzymes are β- mannanases, β-mannosidases and β-glucosidase. This study aimed to isolate and screen for mannanase-producing yeasts from citrus wastes and to produce, optimize, purify and characterize the enzyme. One hundred and two (102) yeasts were isolated from different citrus wastes obtained from five different markets and screened for their mannanase producing ability. Gum Arabic was used as a substrate for the production of mannanase enzyme by yeasts isolates using submerged fermentation. Candida sp. LES2, Candida sp. OS12, Pichia sp. and Pichia kudriavzevii (AUMC 10190) were the yeast isolates with the best potential of mannanase production. Incubation period, pH, temperature, carbon and nitrogen source were optimized under submerged fermentation for the production of mannanase. The mannanase produced by Pichia kudriavzevii (AUMC 10190) was optimally active at pH 7.0 (202.10 U/ml) and its temperature is stable at 60 °C. Pichia kudriavzevii (AUMC 10190) was optimally active at 35 °C (147.91U/ml). Mannanase enzyme produced by Pichia kudriavzevii (AUMC 10190) had a purification fold of 2.14 with a specific activity of 134.53U/ml proving that yeasts obtained from citrus wastes have the ability to produce mannanase enzyme under optimized condition. In conclusion, Pichia kudriavzevii (AUMC 10190) isolated from citrus waste is potential yeast for the production of mannanase under suitable condition for higher yield.
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