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1

Transformacja Arabidopsis thaliana z udzia?em Agrobacterium tumefaciens nios?cym geny mirozynazy TGG1 lub TGG2

100%
Troczynska J., Drozdowska L.
Biotechnologia
|
2007
|
issue 4
170-179
EN
In order to obtain the plants with altered myrosinase activity, the transformation via Agrobacterium tumefaciens was performed. The myrosinase genes TGG1 or TGG2 from Arabidopsis thaliana under the control of enhanced 35S promoter and phosphotranspherase neomycin II (NPTII) as the selectable marker were introduced into Arabidopsis. The presence and expression of NPTII gene in selected plants was confirmed by ELISA and PCR analysis. The integration of T-DNA with TGG1 or TGG2 gene caused differentiated effects from a multi-fold increase of myrosinase activity to its lack. No morphological differences were observed between transgenic and control plants. All the plants were fertile and produced seeds. The majority of the plants with additional copy (copies) of TGG1 showed increased enzyme activity. Conversely, most of the plants with insertion of additional copy (copies) of TGG2 demonstrated decreased myrosinase activity. Transgenic plants may be used in further studies explaining the physiological role of glucosinolates in plant-pest interaction and the importance of the myrosinase-glucosinolates system in growth and development.
2

Induction of glucotropaeolin biosynthesis and myrosinase activity in hairy roots of nasturtium

75%
Wielanek M., Urbanek H.
Biotechnologia
|
2000
|
issue 4
123-127
EN
Kinetin, BAP and NAA increased glucotropaeolin content in dry weight of nasturtium hairy roots but they inhibited biomass growth. Salicylates most strongly (by about 100-200% above control) and DL-?-aminobutyric acid and methyl jasmonate to a lesser degree increased glucotropaeolin yield. They also slightly increased myrosinase activity. Trans-cinnamic acid was a much stronger inducer of myrosinase activity than glucotropaeolin biosynthesis: it strongly inhibited biomass growth. L-1-amino-2-phenylethylphosphonic acid, inhibitor of L-phenylalanine ammonia-lyase (PAL), induced glucotropaeolin production and enhanced the effect of salicylates and jasmonate on glucotropaeoplin yield but it did not affect myrosinase activity.
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