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1

Correlation between porcine PPARGC1A mRNA expression and its downstream target genes in backfat and longissimus dorsi muscle

100%
Erkens T., Vandesompele J., Van_ Z. A., Peelman L. J.
Journal of Applied Genetics
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2009
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vol. 50
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issue 4
361-369
EN
Knowledge of in vivo relationship between the coactivator PPARGC1A and its target genes is very limited, especially in the pig. In this study, a real-time PCR experiment was performed on longissimus dorsi muscle (MLD) and backfat with 10 presumed PPARGC1A downstream target genes, involved in energy and fat metabolism, to identify possible relationships with PPARGC1A mRNA expression in vivo in the pig (n = 20). Except for UCP3 and LPL, a very significant difference in expression was found between MLD and backfat for all genes (P < 0.01). Hierarchical cluster analysis and the significant pairing of mRNA expression data between sampling locations suggested a genetic regulation of the expression of several target genes. A positive correlation with PPARGC1A was found for CPT1B, GLUT4, PDK4, and TFAM (P < 0.0001). A negative correlation was found for UCP2, FABP4, LEP (P < 0.0001), and TNF (P = 0.0071). No significant correlation was detected for UCP3 and LPL. This study provides evidence for a clear difference in mRNA expression of crucial genes in fat and energy metabolism between 2 important tissues. Our data suggest a clear impact of PPARGC1A on energy and lipid metabolism in vivo in the pig, through several of these downstream target genes.
2

GM-CSF regulation in eosinophils

100%
Esnault S., Malter J. S.
Archivum Immunologiae et Therapiae Experimentalis
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2002
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vol. 50
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issue 2
121-130
EN
Allergic asthma is characterized by a temporally and quantitatively inappropriate immunologic response. One of hallmarks of this response is the accumulation of eosinophils in the airway and lung parenchyma, which results in bronco-constriction, lung damage and ultimately fibrosis. GM-CSF plays a pivotal role in this process by modulating eosinophil function and survival. In this review, we discuss the effects and molecular regulation of GM-CSF secretion by eosinophils. Recent data demonstrate activated eosinophils release small amounts of anti-apoptotic GM-CSF by stabilizing its coding mRNA.
3

A novel sheep gene, MMP7, differentially expressed in muscles from black-boned sheep and local common sheep

88%
Yonggang L., Shizheng G.
Journal of Applied Genetics
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2009
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vol. 50
|
issue 3
253-256
EN
Differences in gene expression in muscles from Chinese black-boned sheep and local common sheep were investigated using mRNA differential display. One differentially expressed novel gene was identified through semi-quantitative RT-PCR, and the full-length cDNA sequence was then obtained using the rapid amplification of cDNA ends (RACE). The nucleotide sequence of this gene is not homologous to any of the known sheep genes, but it contains an open reading frame encoding a protein of 416 amino acids, which has high homology with matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) of 10 species: bovine (93%), rhesus monkey (75%), human (74%), pig (73%), chimpanzee (73%), dog (73%), horse (72%), mouse (66%), rat (65%), and chicken (53%). Thus the novel gene can be defined as the sheep MMP7 gene. It was finally assigned to GeneID:100192317. The phylogenetic tree analysis revealed that the sheep MMP7 gene is closely related to the bovine MMP7. Our experiment is the first one to establish the primary foundation for further research on the sheep MMP7 gene.
4

Myosin heavy chain protein and gene expression in the masseter muscle of adult patients with distal or mesial malocclusion

88%
Gedrange T., Buttner C., Schneider M., Oppitz R., Harzer W.
Journal of Applied Genetics
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2005
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vol. 46
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issue 2
227-236
EN
The aim of this study was to determine the amount of myosin heavy chain (MyHC) proteins and MyHC mRNA in muscles of patients with different positions of the mandible. Ten adult patients for orthognathic surgery were divided into two groups: distal and mesial malocclusion. The mRNA expression of two MyHC isoforms of the anterior and posterior part of the right and left side of the human masseter muscle was analysed with a competitive RT-PCR assay. An exogenous template that includes oligonucleotide sequences specific for sarcomeric MyHC isoforms (1 and 2x) was constructed and utilized as competitor. Different isoforms of the MyHC protein were identified by Western blot analysis. In the total mRNA pool of the masseter muscle, the MyHC 1 mRNA level was 25.5 ? 7.6% and the MyHC 2x mRNA was 2.5 ? 1.2%. The anterior part of the masseter muscle from patients with distal occlusion contained more type 1 and 2x MyHC mRNA, as compared to patients with mesial occlusion (P < 0.05). No difference in the protein distribution was observed. The differences in mRNA expression may be caused by the enforced stress of the masticatory muscle in distal occlusion because of the disadvantageous pivot.
5

The action of antidepressant drugs administered during calcium channel blockade

75%
Vetulani J.
|
EN
This is an overview of the symposium concerned with the action of electroconvulsive treatment and some antidepressant drugs (mainly imipramine) applied during blockade of voltage-dependent calcium channels with nifedipine. The results in general suggest that a combination of calcium channel blockers with antidepressant drugs of ECT may be of clinical value in treatment of depression.
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