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Biological databases and their application for functional comparative analysis of organisms ? selected issues

100%
Slesak I., Karpinski S.
Biotechnologia
|
2010
|
issue 4
39-52
EN
A quickly increasing amount of biological data, such as DNA/RNA and protein sequences, determines fundamental role of biological databases for functional comparative analysis of genomes/metabolic pathways of various organisms. This is neatly illustrated by ?Databases', the new type of scientific journal that has recently appeared. The journal is dedicated to bioinformatic data processing and storage. Biological databases and platforms play a crucial role in the development and progress of the systems biology. Systems biology integrates key elements of an organism functions e.g. gene and protein content, regulation of gene expression, enzymes activities, and specific profiles of different metabolites. Therefore, it is possible to generate a holistic and integrated model of basic biological functions and processes that determine an organism phenotype.
2

Isolation and cultivation of endosymbiotic alga from green hydra and phylogenetic analysis of 18S rDNA sequences

88%
Kovacevic G., Franjevic D., Jelencic B., Kalafatic M.
Folia Biologica
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2010
|
vol. 58
|
issue 1-2
135-143
EN
Symbiotic associations are of wide significance in evolution and biodiversity. The green hydra is a typical example of endosymbiosis. In its gastrodermal myoepithelial cells it harbors the individuals of a unicellular green algae. Endosymbiotic algae from green hydra have been successfully isolated and permanently maintained in a stable clean lab culture for the first time.We reconstructed the phylogeny of isolated endosymbiotic algae using the 18S rRNA gene to clarify its current status and to validate the traditional inclusion of these endosymbiotic algae within the Chlorella genus. Molecular analyses established that different genera and species of unicellular green algae could be present as symbionts in green hydra, depending on the natural habitat of a particular strain of green hydra.
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