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1

Modification of collagen film by certain chemical agents

100%
Lesiak-Cyganowska E., Sladowski D., Komender J.
Archivum Immunologiae et Therapiae Experimentalis
|
2001
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vol. 49
|
issue 3
247-251
EN
Morphological changes and the content of free carboxyl groups in bovine collagen (type I) film under the influence of trypsin, hydrochloric acid (HCl) and ethylenediaminetetraacetic acid (EDTA) were studied. Incubation with trypsin and HCl was found to cause some delamination of the film and the appearance of some low-density spots. Incubation with EDTA did not cause any morphological changes. A high concentration of free carboxyl groups (10-fold higher than in control) was seen after incubation with trypsin.
2

In vitro culture of human epithelial cells on a modified xenogenic collagen support

80%
Lesiak-Cyganowska E., Sladowski D., Jankowska E., Komender J.
Archivum Immunologiae et Therapiae Experimentalis
|
2001
|
vol. 49
|
issue 3
253-259
EN
Human epithelial cells (HeLa, HaCaT, NHK) were cultured in vitro on chemically modified collagen membranes. Adhesion to the support was measured by estimation of the percentage of adhering 51Cr-labeled cells. Proliferation was estimated with the XTT test. Morphological observations of cells growing on HCl-treated collagen were performed using histological and electron microscopic techniques. HCl and trypsin-modified xenogenic collagen was found to be a good support for human cells in vitro. EDTA-incubated collagen enhanced neither adhesion nor proliferation. The best adhesion and proliferation were found on HCl-treated collagen, depending, however, on the kind of cells.
3

50 years of studies of restriction ? modification systems

80%
Waleron K., Nakonieczna J., Waleron M., Podhajska A. J.
Biotechnologia
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2006
|
issue 2
65-88
EN
The basic function of restriction endonucleases and methyltransferases is protection of the host genome against foreign DNA. Their recombination and transposition related functions are still being discussed. Some authors postulate that R-M genes may act as selfish genetic elements. Restriction endonucleases are indispensable tools in molecular biology. As these enzymes recognize DNA sequence very specifically, they serve as a model for protein-DNA interaction. Restriction-modification genes have also played the same role as a model for evolutionary studies as well as protein structure - function relations. So far, there have been more than 3500 bacterial strains studied which possessed R-M genes of more than 280 different specificities towards recognition sequence and cleavage sites. They became a very good commercial product for many biotechnological companies. At present, in a genome sequencing era, R-M genes seem to be much more common than it was thought before.
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