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EN
Plants produce a vast array of secondary metabolites. There are three major groups of them: terpenes, nitrogen-containing secondary metabolites and phenolics. These molecules have a great impact on biology of plants and environment. Here, we briefly introduce flavonoids ? one of the largest class of plant phenolics. Their biosynthesis, properties and different functions are presented. Special attention is paid to legume plants and a role of flavonoids as signaling molecules in symbiosis. For human nutrition, flavonoids represent compounds with health-promoting activities and such properties for some of them are also indicated.
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issue 4
82-108
EN
Plant secondary products are the substances of great importance in many spheres of human life. In recent years, many methods have been investigated in order to increase yield of these compounds, synthesized in plant in vitro cultures ? systems, which proved to be very useful and efficient for this purpose. Among these techniques, biotic elicitation, although not yet applied to a large scale production, proved to be a very efficient procedure on laboratory scale. One of the major aims of the studies on biotic elicitation of plants is to identify universal and effective, but at the same time the cheapest and simplest elicitors which could be used to increase secondary metabolites' production in plant in vitro cultures. This review focuses on different biotic elicitors of complex composition (e.g.: fungal culture filtrates and homogenates), as well as those with a known structure (e.g.: chitosan or ergosterol). The factors influencing the elicitation process as well as ways of improving efficiency of this method by combining it with other techniques, which can also increase plant tissue productivity, are also discussed.
EN
Modern biotechnology is looking for a new source of high quality pharmaceuticals. Interestingly, many of such pharmaceuticals are often natural products provided by plants. An example is a vast array of secondary metabolites. Apart from extraction of these compounds from plant material, many different strategies have been proposed to efficiently produce some of them, using suspension cell cultures. Plants are also a good source of many recombinant proteins, offering a possibility of posttranslational modifications which are limited in prokaryotes. Efficient systems of stable and transient heterologous protein expression for the industrial scale were developed. Here, we briefly introduce such strategies and give examples of different plant products obtained by applying them.
EN
Propentofylline (PPT) is a drug used in the treatment of both vascular dementia and Alzheimer type dementia. Hydroxy-metabolites of propentofylline (OHPPT) also demonstrate the same biological activity as the parent compound. As steroisomers of HOPPT are not commercially available, we had to produce them for pharmacological and pharmacokinetic studies. The aim of this study was to find the strains of Saccharomyces cerevisiae yielding enantiomerically pure (R)- or (S)-1-(5-hydroxyhexyl)-3,5-dimethyl-7-propylxanthin (OHPPT) from PPT. In this paper, we present the results of stereoselective reduction of PPT into OHPPT when catalysed by whole cells of baker's and a few strains of wine yeast in water and organic solvents.
EN
Transformed roots are also called transgenic roots or due to their appearance ? hairy roots. Transformed roots are obtained by infection of plants or parts of them with soil bacteria, Agrobacterium rhizogenes. Genetically transformed roots are characterised by rapid and stable growth of biomass, genetic stability, and stable biosynthesis of metabolites. Hairy roots can be an alternative source of many economically important chemicals like enzymes, pigments, flavours and especially bioactive pharmaceuticals. Cultures of transformed roots are comparatively easy to cultivate in shaking flasks. Application of flasks does not allow to obtain great amount of biomass. There is a necessity of scaling up the culture using bioreactors. The first attempts of scaling up the culture were connected with bioreactors designed for microbiological processes. However, these bioreactors are inadequate for hairy roots cultures. Some promising results were achieved in the case of using mist bioreactor. This paper is a review of bioreactors employed in cultures of hairy roots and mathematical modelling of hairy root growth.
EN
This article summarizes the medical, ecological, industrial and biotechnological importance of actinomycetes. At present, according to a new hierarchic classification system, they are the most representative group of Actinobacteria classis nov. These unusual microorganisms with such rich biological activity have had a confusing history and classification for over a hundred years. In this review, following subjects have been raised: important, but less known human, animal and plant pathogens as well as actinomycete-diseases; symbionts as endophytes in nitrogenase activity; agents of biological control; biodeterioration and biodegradation; primary and secondary metabolites including their biological, industrial and biotechnological importance. Additionally, some lipid compounds of cell wall as immunomodifiers have been also mentioned. Actinomycetic activities have always had far-reaching effects, and these are sure to remain in different guises.
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