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1

Mathematic's models in metabolic engineering

100%
Szewczyk K. W.
Biotechnologia
|
2005
|
issue 2
7-31
EN
Metabolic engineering is an integrating methodology of analysis and synthesis for improvement of flux distribution of metabolic pathways. It has two main aspects: modeling and analysis of metabolic networks to establish strategies for pathway engineering and actual molecular level engineering the pathway. Mathematical modeling is one of the key methodologies of metabolic engineering. The review presents the currently used metabolic modeling approaches. Metabolite balancing is the basis for analysis of metabolic flux and cell capability to form a targeted product. The use of isotope ? labeled substrates with nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GCMS) analyses of intracellular and extracellular metabolites enables determination of metabolic flux distribution. Metabolic Control Analysis (MCA) is a theoretical framework for investigation of control mechanism of metabolic network to identify key parameters influencing productivity. Kinetics models present a more detailed approach to simulate metabolic net behavior. Linear approximation of kinetic model, the so called (log)linear kinetics, is useful for modeling spatiotemporal variations of the net. The including of genetic regulation to metabolic models, the next step in the development of metabolism models, needs new methods of experimental approaches and mathematic and computational resources. Metabolic engineering is barely a decade old, but its significance is already widely recognized in the research-intensive biotechnology community and attracts great interest of industry.
2

Metabolic engineering of microorganisms for construction of efficient bioethanol producers from lignocellulose

88%
Sybirny W., Puchalski C., Sybirny A.
Biotechnologia
|
2007
|
issue 4
38-54
EN
In the review, the current state of the art, problems and perspectives in the development of the economically feasible production of fuel ethanol from a plant biomass (lignocellulose) are presented. The metabolic engineering of microorganisms directed to design the strains with the improved ability to alcoholic fermentation (expansion of a spectrum of fermenting substrates, increase in the fermentation rate and the yield of ethanol, tolerance to ethanol and the inhibitors present in lignocellulose hydrolyzates, thermotolerance, ability to simultaneous enzymatic hydrolysis of cellulose and hemicelluloses together with alcoholic fermentations of hexoses and penthoses) are considered. The information about the new pilot plants on fuel ethanol production from lignocellulose, which were recently started up in Canada and Sweden is presented. Justification of carrying out the corresponding studies in Poland is discussed.
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