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1

Animal genome mapping projects

100%
Fredholm M.
Biotechnologia
|
1996
|
issue 2
13-19
EN
During the latest years many examples have been provided illustrating that gene maps can be utilized fer search aiming at (i) isolation causing oe having an influence on certain diseases, (ii) studying the genomic organization an evolutionary relationship of mammalian species, and (iii) eveloping animal models of human disease.
2

Chromosomal assignment of R-spondin genes in the donkey (Equus asinus, 2n = 62)

80%
De_ L. L., Genualdo V., Perucatti A., Pia_ D. M. G., Molteni L., Iannuzzi L., Parma P.
Journal of Applied Genetics
|
2010
|
vol. 51
|
issue 3
319-321
EN
R-spondins constitute a recently discovered small family of growth factors, and the evidence of their role in several developmental pathways is growing fast. In this work we describe the chromosomal location of the four RSPO genes in the donkey. Using horse BACs, we localized RSPO1 on EAS 5q23, RSPO2 on EAS 12q13, RSPO3 on EAS 24q26, and RSPO4 on EAS 15p13. Moreover, RSPO2, RSPO3, and RSPO4 are the first genes mapped on donkey chromosomes 12, 24, and 15, respectively.
3

Unidirectional orientation of twelve expressed tagged sites within 40 kb of human chromosomal region 22q13.1

80%
Pusch C., Wang Z., Roe B., Blin N.
Journal of Applied Genetics
|
1998
|
vol. 39
|
issue 2
199-204
EN
. The single copy sequence D22S16 from human chromosomal region 22q13.1 that carries a putative conserved gene, was used to probe a chromosome 22-specific cosmid library. Genomic sequencing of one positive, 40 kb long cosmid (C1155) revealed a hereto unmapped gene (a subunit of DNA-dependent RNA polymerase II, POLR2F), a SOX9-related sequence and 12 expressed sequence tags. Although not parts of one consecutive gene, all 12 ESTs and, in addition, the polymerase gene are oriented in the same transcriptional direction within the genomic sequence represented by cosmid C1155.
4

Application of a mixed inheritance model to the detection of quantitative trait loci in swine

80%
Szyda J., Liu Z., Grindflek E., Lien S.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 1
69-83
EN
The primary goal of this study was to investigate statistical properties of a mixed inheritance model for the localization of quantitative trait loci (QTL). This is based on the analysis of phenotypic data for the amount of intramuscular fat (IMF) scored on 305 individuals originating from a cross between Duroc and Norwegian Landrace breeds. Marker genotype information is available for F1 and F2 generations. Statistical procedures compared involve i) the interval mapping, ii) the composite interval mapping, iii) a regression method, and iv) a mixed inheritance model accounting for a random animal additive genetic effect and relationships between individuals. The basic statistical properties of the latter approach are then assessed using Monte Carlo simulations showing slight unconservativeness as compared to and reasonable power to detect QTL of moderate effects. In the analysis of IMF data, the significant evidence for the existing QTL is detected on chromosome 6. A chromosomal region recommended for a second-step fine mapping analysis is identified between markers SW1823 and S0228, based on three types of confidence intervals derived by using: i) the Jackknife algorithm, ii) the numerical variance approximation, and iii) the LOD score approach. The Jackknife algorithm was additionally used to quantify each family?s contribution to the test statistic and to the estimate of QTL position.
5

Genetic mapping of the gene affecting polyphenol oxidase activity in tetraploid durum wheat

70%
Watanabe N., Masum A. A. S. M. G., Nachit M. M.
|
|
issue 3
201-205
EN
The quality of durum wheat (Triticum turgidum ssp. durum) is influenced by polyphenol oxidase (PPO) activity and its corresponding substrates. A saturated molecular-marker linkage map was constructed previously by using a set of recombinant inbred (RI) lines, derived from a cross between durum wheat cultivars Jennah Khetifa and Cham 1. Quantitative trait loci (QTL) for PPO activity in seeds were mapped in this population. PPO activity in seeds of the parents and 110 RI lines was measured spectrophotometrically. The PPO activity of Cham 1 was significantly lower than that of Jennah Khetifa. QTL analysis of these data indicated that most of PPO activity was associated with major loci on the long arm of chromosome 2A. The trait was found to be strongly associated with the SSR marker Xgwm312@2A. With this knowledge, marker-assisted selection can be used to select genotypes with lower PPO activity in durum wheat populations.
6

Interval mapping of QTLs controlling yield-related traits and seed protein content in Pisum sativum

61%
Irzykowska L., Wolko B.
Journal of Applied Genetics
|
2004
|
vol. 45
|
issue 3
297- 306
EN
A linkage map of garden pea was constructed on the basis of 114 plants (F2 generation) derived from a cross combination Wt10245 ? Wt11238. The map, consisting of 204 morphological, isozyme, AFLP, ISSR, STS, CAPS and RAPD markers, was used for interval mapping of quantitative trait loci (QTLs) controlling seed number, pod number, 1000-seed weight, 1000-yield, and seed protein content. Characterization of each QTL included identification of QTL position with reference to the flanking markers, estimation of the part of variance explained by this QTL, and determination of its gene action. The yield-related traits were measured in F2 plants and in F4 recombinant inbred lines (RILs). The interval mapping revealed two to six QTLs per trait, demonstrating linkage to seven pea chromosomes. A total of 37 detected QTLs accounted for 9.1-55.9% of the trait's phenotypic variation and showed different types of gene action. As many as eight and ten QTLs influencing the analysed traits were mapped in linkage groups III and V, respectively, indicating an important role of these regions of the pea genome in the control of yield and seed protein content.
7

The transpositional elements as a tool for plant genome mapping

51%
Wochniak P., Malepszy S.
Biotechnologia
|
1994
|
issue 3
15-29
EN
This work aims at reviewing current progress in the field of plant transposble elements, especially those described as Ac/Ds and En/dSpm systems, first discovered in maize.We gave the molecular characteristics of plant transposones and the rules of their behaviour within a genome.The procedure for a particular gene mapping and for mapping of many genes responsible for biochemical pathway were cited.In comparison with other genome mapping methods the advantages and drawbacks of "gene tagging" were envisaged.The enclosed tables provide many documental examples of plant genes identification via "gene tagging" method.
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