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1

A simple and reliable method for DNA extraction from bivalve mantle

100%
Aranishi F., Okimoto T.
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issue 3
251-254
EN
A simple and reliable method was developed for extracting genomic DNA from preserved mantle tissues of Pacific oyster Crassostrea gigas for reproducible PCR amplification. The method was based on destruction of the tissue using Proteinase K, Chelex 100 resin, detergents, and urea, followed by preferential capturing of genomic DNA with silica particles. Approximately 5 mg of mantle tissue provided a sufficient quality and quantity of DNA for several hundreds of PCR reactions amplifying the hypervariable mitochondrial DNA intergenic spacer, which is a useful genetic marker for population structure analysis of Pacific oyster. The method can be applied for DNA preparation from not only fresh and frozen but also ethanol-preserved mantle tissues, so this rapid and economical method can serve for investigating a large number of bivalve specimens collected in the field and next transported in ethanol at ambient temperature.
2

Distribution of major lipids and fatty acids of the estuarine gastropod mollusc Telescopium telescopium

75%
Rakshit S., Bhattacharyya D. K., Misra K. K.
Folia Biologica
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1997
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vol. 45
83-87
EN
Lipid fractions and fatty acid composition of different organs, viz., digestive gland, mantle, and foot of the gastropod Telescopium telescopium inhabiting estuarine waters of West Bengal, India were studied. Thin layer chromatography and subsequent colorimetric analyses revealed the presence of phospholipid, cholesterol, and, to a lesser extent, triglycerides. Considerable variation in the three classes of lipids was observed in the organs studied so far. Gas liquid chromatography analyses showed the presence of saturated fatty acids (16: 0, 18: 0, 14:0), monoenoics (18:1, 16:1, 20:1), and polyenoic fatty acids (22:5 w6, 20:5 w3, 18:2) in varying amounts in the three organs. This may be related with a specific function of the organ. Polyunsaturated fatty acids are present in fair amounts in T. telescopium. The occurrence of arachidonic acid (20:4) in this gastropod may be related to salinity, as was pointed out by other workers.
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