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1

The effect of 2,4-D concentration and osmotic stress on the androgenic response in anther culture of barley

100%
Kruczkowska H., Pawlowska H., Skucinska B.
Biotechnologia
|
2004
|
issue 2
244-250
EN
Induction of androgenesis in in vitro microspore culture of barley is the most efficient method of haploidisation, but it is difficult to use in the conditions of a plant breeding station. For this reason, the method has been improved side by side with the technique of anther culture. In anther culture of 3 barley cultivars, pretreatment with 0.7 mol dm-3 mannitol and the addition of 0.2 mg dm-3 2,4-D to the induction medium have been shown to exert a favourable effect. In cv. Mobek, the interaction of the two tested stress agents, mannitol and auxin, has been proved.
2

Genotypic veriation in response to iso-osmotic and soil moisture stresses among old and modern cultivars of winter wheat

63%
Mendel M., Gorny A.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 1
49-63
EN
The study was performed to evaluate range of the genotypic variation in drought susceptibility at juvenile growth stages among 23 old and modern winter cultivars of Triticum aestivum and one old cv. of T.spelta.Response of germinated seeds to mannitol-simulated drought stress as well as that in seedlings shoot and root growth, water use and leaf transpiraton under varied soil moisture were measured.Considerable genotypic variation for all morpho-physiological traits was found between wheat cultivars.There were significant differences between the older and modern cultivars.The former showed usually an enhanced rooting ability and higher water requirements, while the never indicated more efficient water use.The older cultivars were more resistant to both iso-osmotic stress and low soil moisture at the juvenile growth stage.However, no clear differences were found between the two groups of cultivars in the variation range of the drought susceptibility indices.Complex patterns of the response to drought were observed.Cultivars, which distinguished by both a lower shoot:root ratio and a stronger drought-induced stimulative root growth, tended to be more resistant.Although only moderate the relationships noticed, a consideration of the plasticity screening in local wheat breeding appears to be justified.
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