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vol. 34
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issue 3
237-247
EN
Experiments were carried out on (cvs. Wat and Hetman).L.luteus (cvs. Topaz and Iryd), (cv, Remik), and . For culture initiation 5- and 14-day-old seedling explants were used. The of the was stimulated by various media but the universal one appeared to be Gamborg's B5 medium solidified with Gelrite. For each investigated species a medium assuring 100% rooting of explants was developed. The most effective combination of growth substances for multiplication of L. hispanicus was found. Cotyledonary node explants, cultured on medium supplemented with benzyladenine and with or without naphthaleneacetic acid were stimulated to undergo in 4 and 2, for L. hispanicus and L. albus, respectively. Plantlets obtained from all experiments were easily potted.
EN
Observations of a specific rDNA locus behaviour during the cell cycle were made by fluorescent in situ hybridisation (FISH) in 12 Lupinus species. Due to the pattern of chromatin de-condensation in that locus, the number of relevant sites in interphase nuclei was twice as high as the number of signals on metaphase chromosomes. The description of successive phases and an attempt of an explanation are given.
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