Search results

Search:
in the keywords: LOLIUM

Sort By:

Limit search:

Variability of ribosomal DNA sites in Festuca pratensis, Lolium perenne, and their intergeneric hybrids, revealed by FISH and GISH

100%

Ksiazczyk T., Taciak M., Zwierzykowski Z.

This study focuses on the variability of chromosomal location and number of ribosomal DNA (rDNA) sites in some diploid and autotetraploid Festuca pratensis and Lolium perenne cultivars, as well as on identification of rDNA-bearing chromosomes in their triploid and tetraploid F. pratensis x L. perenne hybrids. The rDNA loci were mapped using fluorescence in situ hybridization (FISH) with 5S and 25S rDNA probes, and the origin of parental genomes was verified by genomic in situ hybridization (GISH) with L. perenne genomic DNA as a probe, and F. pratensis genomic DNA as a block. FISH detected variation in the number and chromosomal location of both 5S and 45S rDNA sites. In F. pratensis mostly additional signals of 5S rDNA loci occurred, as compared with standard F. pratensis karyotypes. Losses of 45S rDNA loci were more frequent in L. perenne cultivars and intergeneric hybrids. Comparison of the F. pratensis and L. perenne genomes approved a higher number of rDNA sites as well as variation in chromosomal rDNA location in L. perenne. A greater instability of F. pratensis-genome-like and L. perenne-genome-like chromosomes in tetraploid hybrids was revealed, indicating gains and losses of rDNA loci, respectively. Our data indicate that the rDNA loci physically mapped on chromosomes 2 and 3 in F. pratensis and on chromosome 3 in L. perenne are useful markers for these chromosomes in intergeneric Festuca x Lolium hybrids.

Interspecific and intergeneric hybrids of the Lolium-Festuca complex obtained in Poland in the years 1964-1994 and maitained in the collection at the Institute of Plant Genetics in Poznan

75%

Zwierzykowski Z.

In the years 1964-1994 an extensive programme of wide hybridization within the Lolium-Festuca complex was carried out in Poland.Six Lolium (ryegrass) and five Festuca (fescue) species at differnt ploidy level were used for crosses.Hybrids were obtained from 72 cross combinations.This article presents a complete list of Folium-Festuca hybrids obtained in Poland in the years 1964-1994 and maintained in the collection of Institute of Plant Genetics in Poznan.The available literature concerning these hybrids is cited.

Refine search results

2 Journal of Applied Genetics

1 2010

1 1996

2 Zwierzykowski Z.

1 Ksiazczyk T.

1 Taciak M.

Search results

Variability of ribosomal DNA sites in Festuca pratensis, Lolium perenne, and their intergeneric hybrids, revealed by FISH and GISH

Interspecific and intergeneric hybrids of the Lolium-Festuca complex obtained in Poland in the years 1964-1994 and maitained in the collection at the Institute of Plant Genetics in Poznan