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1

Simultaneous detection of malignant hyperthermia and genetic predisposition for improved litter size in pigs by multiplex PCR-RFLP

100%
Omelka R., Vasicek D., Martiniakova M., Bulla J., Bauerova M.
Folia Biologica
|
2004
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vol. 52
|
issue 1-2
113-115
EN
The ryanodine receptor gene (RYR1) and the estrogen receptor gene (ESR) are the best commercially used markers for predisposition of stress susceptibility (malignant hyperthermia ? MH) and increased litter size, respectively. A simplified method of simultaneous detection of MH and ESR genotypes has been developed. The method is based on simultaneous amplification of fragments of two genes by multiplex PCR and subsequent digestion of the products with two restriction enzymes. The PCR and the digestion could be performed in a single tube and all genotypes could be detected by electrophoretic separation on the same agarose gel. Thus, the development of the method can decrease the cost of the sample analysis and increase the speed and efficiency of the analysis. In our study, frequencies of mutated T allele of the RYR1 gene in Large White (LW), White Meaty (WM) and Landrace (L) were 0.11, 0.13, and 0.15, respectively. Frequencies of the preferred B allele of the ESR gene in the same breeds were 0.35, 0.26, and 0.06, respectively.
2

Decrease in pig fertility as a result of reciprocal translocations and associated economic effects on the basis of rcp(7;13)(q13;q46)

88%
Danielak C. B., Kozubska S. A., Slota E., Rejduch B.
Journal of Applied Genetics
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1996
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vol. 37
|
issue 4
373-384
EN
Reciprocal translocations, very frequently identified in pigs, are the cause of fertitlity decrease. The aim of this work was to provide an objective assessment of the real effect of reciprocal translocation (7;13) on the fertility of carriers and associated economic effects. The experiment has shown that fertility, expressed as a mean litter size, decreased by 48% in comparison to that of the control group. On the basis of simulation account, financial losses incurred a translocation as a result of using carrying boar in a commercial herd were estimated at about 8,000 USD for natural mating and at about 162,000 USD for artificial insemination. The results obtained show the need for introducing a system for cytogenetic control of boars producing small litters into the Polish swine improvement programme.
3

Evaluation of the effect of centromeric heterochromatin polymorphism on pig fertility

75%
Kozubska-Sobocinska A., Danielak-Czech B., Slota E., Rejduch B.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 3
293-298
EN
C-banding identified centromeric heterochromatin polymorphism, most often located in a pair no 16, was a basis for selecting animals for the experiment.The aim of the experiment was to assess the impact of centromeric heterochromatin polymorphism on pig fertility, expressed by litter size.The research included the first two litters obtained from 30 pairs of parent with different genotypes as regards the parameter under study.A statistical analysis of the number of offspring from different mating types showed no significant differences between the experimental groups of animals.The results obtaned did not confirm suggested correlation between polymorphism of the centromeric heterochromatin regons and pig fertility, estimated by mean size.Thus the polymorphism cannot be regarded as a selection criterion.
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