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1

The effect of Attribut 70 WG herbicyde on physiological activity of selected entomophagous fungi

100%
Mazurkiewicz-Zapalowicz K., Michalcewicz W., Mietkiewski R., Janowicz K., Daraz L.
Biotechnologia
|
2004
|
issue 1
201-209
EN
In vitro examination has been carried out to determine the influence of sodium propoxycarbazone, an active ingredient of Attribut 70 WG herbicyde, in a dose of 1,10,100 and 1000 ppm on the growth, sporulation, respiration and lipolytic and proteolytic activities of Beauveria brongniartii (Sacc.) Petch and Metarrhizium flavoviride Gams et Rozsypol mycelia. A specific response of each strain to the contact with the tested herbicyde was found. Higher concentrations of Attribut 70 WG (100 and 1000 ppm) stimulated M. flavoviride sporulation, whereas they significantly reduced the reproductive potentials of B. brongniartii. However, the contact of B. brongniartii with higher doses of Attribut 70 WG had an effect on significant increase of lipolytic and proteolytic activities of that entomophage. Such a response is very indicated for soil ecosystem, as it may be employed in biological plant protection in which B. brongniartii is a very valuable fungi species that produces epizootics of numerous plant pest insects.
2

Influence of substrate on fluctuation of lipolytic activity and parameters of activated sludge

100%
Wiechetek A., Turek S. J., Choinski D., Georgi M., Miksch K.
Biotechnologia
|
2008
|
issue 3
159-173
EN
Lipids are important and burdensome organic constituents of most wastewater. The amount of lipids in municipal wastewater is approximately 30-40% of the total organic matter, measured as chemical oxygen demand (COD). Lipids can form oil films on the surface of activated sludge flocks, preventing the diffusion of oxygen and causing problems in the pumping and aeration systems with development of filamentous microorganisms. The current practice to improve the biodegradation of lipids is bioaugmentation, with the addition of microorganisms or enzyme preparations for wastewater treatment. Influence of substrate on fluctuation of lipolytic activity and parameters of activated sludge were studied. The activated sludge was bred from biological uses to bioaugmentation process. Enzymatic activities of activated sludge formed the schema: first, the maximum of lipolytic activity with respirometric activity were observed, next, the highest was dehydrogenases activity. It could be caused by a change of the metabolic path in Pseudomonas fluorescens cells, which includes replacement of TCA cycle by glyoxylate cycle. This replacement requires higher metabolic energy, so high lipolytic activity was connected with respirometric activity. The influence of pH on enzymatic activity of activated sludge was studied. The most effective method to remain stable pH in the chamber of reactor (6,5 ? 7,0) was buffered inflow with KH2PO4/NaOH.
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