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1

Application of STS markers for leaf rust resistance genes in near-isogenic lines of spring wheat cv. Thatcher

100%
Chelkowski J., Golka L., Stepien L.
Journal of Applied Genetics
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2003
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vol. 44
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issue 3
323-338
EN
Sequence tagged site (STS) markers for eight resistance genes against Puccinia recondita f. sp. tritici were used to screen a set of near-isogenic lines of wheat cv. Thatcher containing in total 40 different Lr genes and their alleles. Polymerase chain reaction (PCR) analysis was carried out by using STS, SCAR and CAPS primers specific for the leaf rust resistance genes Lr1, Lr9, Lr10, Lr19, Lr24, Lr28, Lr37 and Lr47. The STS, CAPS and SCAR markers linked to resistance genes Lr9, Lr10, Lr19, Lr24, Lr37 and Lr47 were found to be reliable in diverse genetic backgrounds. The amplification product of the Lr1 gene marker was detected in the susceptible cv. Thatcher and in all of the near-isogenic lines examined except Lr2a, Lr2b, Lr2c and Lr19. The sequence analysis of PCR products amplified in lines Lr1, Lr10, Lr28 and in cv. Thatcher indicated that the near-isogenic lines and cv. Thatcher contained in the targeted chromosome region an allele that differed from the original alleles corresponding to Lr1/6*Thatcher (TLR621) and susceptible Thatcher (TH621). The amplification product specific to the STS marker of the Lr1 gene was amplified in almost all Thatcher near-isogenic lines and in cv. Thatcher because their alleles possessed primer sequences identical to the original allele TLR621. The marker for the Lr28 resistance gene was identified in line Lr28, carrying gene Lr28, and in 21 other near-isogenic lines. The sequencing of PCR products specific to Lr28 and generated in lines Lr1, Lr10 and Lr28 indicated that the lines Lr1, Lr10 and Lr28 are heterozygous in this region.
2

PstIAFLP based markers for leaf rust resistance genes in common wheat

100%
Blaszczyk L., Tyrka M., Chelkowski J.
Journal of Applied Genetics
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2005
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vol. 46
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issue 4
357-364
EN
The aim of the present study was to detect candidate DNA markers for selected leaf rust resistance genes. A total number of 286 loci in the ?Thatcher' near-isogenic lines carrying resistance gene Lr1, Lr9, Lr10, Lr13, Lr19, Lr21, Lr24, Lr26, Lr28, Lr35, and Lr37 were screened for DNA polymorphism by the PstIAFLP method. A survey with 33 selective primers yielded 16 candidate markers. Further validation studies on cultivars characterized for the presence and absence of selected resistance genes confirmed specificity of markers for Lr24, Lr26 and Lr37. The AFLP-based marker P42-530 was successfully converted into an STS marker. The new marker was linked with the Lr37-specific marker (CslVrga13) at the distance of 1.7 cM. The PstIAFLP method was found to be effective in the identification of DNA changes induced in hexaploid wheat by translocations from Agropyron elongatum, Secale cereale and Aegilops ventricosa.
3

Leaf rust resistance genes of wheat: identification in cultivars and resistance sources

100%
Stepien L., Golka L., Chelkowski J.
Journal of Applied Genetics
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2003
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vol. 44
|
issue 2
139-149
EN
Thirty-seven wheat cultivars originating from seven European countries were examined by using sequence tagged site (STS) markers for seven Lr (leaf rust = brown rust) resistance genes against the fungal pathogen of wheat Puccinia recondita f. sp. tritici (Lr9, Lr10, Lr19, Lr24, Lr26 and Lr37). Additionally, 22 accessions with various Lr genes from two germplasm collections were tested. A Scar (sequence-characterized amplified region) marker for Lr24 and a CAPS (Cleaved Amplified Polymorphic Sequence) marker for Lr47 were also used to identify those genes in the wheat accessions. Each marker amplified one specific DNA fragment. Three Lr gene markers were identified in wheat cultivars (Lr10, Lr26 and Lr37). Another four markers (Lr9, Lr19, Lr24 and Lr47) were found in breeding lines carrying leaf rust resistance genes. The results were compared with leaf rust resistance gene postulations made in previous studies, based on multipathotype testing. Markers for Lr10, Lr26 and Lr37 may be useful in marker-assisted breeding.
4

Resistance of spring wheat cultivars and lines to leaf rust

100%
Wisniewska H., Stepien L., Kowalczyk K.
Journal of Applied Genetics
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2003
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vol. 44
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issue 3
361-368
EN
Spring wheat nursery accessions, including 18 spring wheat lines derived in CIMMYT, Mexico, and 12 spring wheat cultivars bred in Poland, along with cultivars Frontana and Sumai 3 as resistant controls, were examined for resistance to leaf rust under field conditions. Multipathotype tests with 16 different pathogen isolates were performed for postulation of Lr genes in Polish cultivars. Besides, STS markers for resistance genes Lr1, Lr9, Lr10, Lr24, Lr28, Lr37 were analysed in the studied cultivars and lines with Thatcher near-isogenic lines as positive controls. All Polish cultivars appeared to be susceptible to leaf rust. Ten of the CIMMYT nursery lines (IPG-SW: #7, 11, 14, 21, 22, 23, 27, 29, 30, 32) and cv. Frontana were resistant in the same environment and can be sources of resistance genes. Marker for the Lr10 gene was identified in 6 accessions (IPG-SW #14, 22, 23, 29, 30, 32) exhibiting resistance to leaf rust, whereas markers for Lr1 and Lr28 genes were observed in all the examined accessions. STS markers for Lr9, Lr24 and Lr37 genes were not identified in the investigated accessions.
5

Response to stripe rust (Puccinia striiformis Westend. f. sp. tritici) and its coincidence with leaf rust resistance in hexaploid introgressive triticale lines with Triticum monococcum genes

100%
Sodkiewicz W., Strzembicka A., Sodkiewicz T., Majewska M.
Journal of Applied Genetics
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2009
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vol. 50
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issue 3
205-211
EN
Triticale introgressive lines were developed by incorporating diploid wheat (Triticum monococcum [TM16]) genes into the hexaploid triticale genotype LT522/6. The synthetic allotetraploid T. monococcum cereale (AmAmRR) was used as a bridging form to introduce the genes. A group of 43 introgressive lines, parental stocks and a check cultivar were inoculated at the seedling stage (in the greenhouse) and at the adult plant stage (in the field) with four pathotypes of Puccinia striiformis f. sp. tritici to determine if the stripe rust resistance was derived from TM16 and to analyze the expression of the diploid wheat gene(s) at the hexaploid level. At the seedling stage, 14 triticale introgressive lines expressed resistance to some of the used pathotypes, showing introduction of a genetic material from the T. monococcum genome. Among them, 7 lines were resistant to all four stripe rust pathotypes applied at this stage. In the field, adult plant resistance and percentage of infected leaf area were scored and transformed into the coefficient of infection. Plant response to stripe rust was compatible at these two developmental stages with a high statistical probability showing the genetic dependence on the same genetic background. Also observed was a full concordance of the adult plant resistance to stripe rust with previously assessed resistance to leaf rust, as well as the highly significant linkage of the resistance to the both diseases at the seedling stage in the set of the tested introgression lines. This result strongly suggests that T. monococcum genes responsible for these characters are located in proximity.
6

Inheritance of leaf rust resistance in wheat lines carrying Aegilops speltoides Tausch. translocation in Chinese Spring background

88%
Bansal U. K., Saini R. G., Khanna R.
Journal of Applied Genetics
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2008
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vol. 49
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issue 2
141-145
EN
The genetic basis of seedling and adult-plant leaf rust resistance was analysed in wheat lines CS 2A/2M 4/2 and CS 2D/2M 3/8, which are reference lines for the leaf rust resistance gene Lr28. Some seedlings of CS 2A/2M 4/2 were susceptible to Indian Puccinia triticina (Pt) pathotypes 77-1, 77-2 and 77-5. These susceptible seedlings exhibited resistance at the adult-plant growth stage. In contrast, CS 2D/2M 3/8 showed resistance to all Pt pathotypes both at the seedling and adult-plant growth stages. The analysis of inheritance in the susceptible plants of CS 2A/2M 4/2 (CS 2A/2M 4/2 APR selection) and CS 2D/2M 3/8 against Pt 77-5 (the frequently occurring Pt pathotype from the Indian subcontinent), indicated that line CS 2D/2M 3/8 was fixed for a dominant gene, presumed to be Lr28, whereas line CS 2A/2M 4/2 was heterogeneous for Lr28. The adult-plant resistance in the CS 2A/2M 4/2 APR selection was conferred by an unknown recessive gene.
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