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1

Influence of reaction conditions on yield of enzymatic synthesis galactooligosacharides by Penicillium canescens beta-galactosidase

100%
Bednarski W., Kulikowska A.
Biotechnologia
|
2007
|
issue 2
137-148
EN
The aim of the work was to examine the ability of Penicillium canescens beta-galactosidase to synthesize galactooligosacharides in one or two-phase medium (organic solvents: water). The yield of this process depends mainly on: lactose concentration in water phase, time of reaction, kind of organic solvents and their participation in two-phase medium. The application of ultrafiltration for separation of saccharides from the reaction medium stabilized the yield of galactooligosacharides synthesis.
2

Immobilization of recombinant beta-galactosidase in reactions catalysed by transglutaminase

100%
Wolosowska S., Synowiecki J.
Biotechnologia
|
2005
|
issue 3
209-220
EN
Thermostable beta-galactosidase from Escherichia coli transformant containing the enzyme gene from Pyrococcus woesei was immobilized at pH 5.5 on silica gel by crosslinking with transglutaminase. The obtained preparations had a specific activity of 11.573 U/g of support at 70C using oNPG as a substrate. The optimum pH and temperature for immobilized beta-galactosidase activity were 5.5 and 95C. The immobilized enzyme is stable at the temperatures close to the optimal value and the residual activity for oNPG hydrolysis of the preparations incubated 1 h in 0.1 M phosphate citrate buffer (pH 5.5) at 100C was about 70% of the initial value.
3

Improvement of beta-galactosidase properties and conditions for its microbial biosynthesis

88%
Bednarski W., Kowalewska-Piontas J.
Biotechnologia
|
2000
|
issue 2
131-141
EN
We present examples of genetic modification of microorganisms capable of beta-galactosidase synthesis. The technological characteristics as termostability, high activity in a low temperature is improved. We also describe intensification of beta-galactosidase secretion to the medium.
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