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1

Effect of hibernation on sodium and chloride ion transport in isolated frog skin

100%
Kosik-Bogacka D., Tyrakowski T.
|
2007
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vol. 55
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issue 1-2
47-51
EN
The aim of the study was to evaluate the effect of hibernation on electrophysiological parameters of isolated frog skin under control incubation (Ringer solution) and after inhibition of Na+ and Cl- transepithelial transport by application of amiloride and bumetanide. The transepithelial electrical potential difference (PD in mV) was measured before and after mechanical stimulation of isolated frog skin. The tissues were mounted in a modified Ussing chamber. The results revealed a reduced PD of frog skin during hibernation. In February, as compared with November, PD of frog skin incubated in Ringer solution decreased by about 50%. Hibernation also affected hyperpolarization (dPD) of frog skin after mechanical stimulation. In November and December, dPD was about 50% and 30% lower, respectively, compared with the subsequent two months of the experiment. The incubation of frog skin with amiloride, a sodium ion channel blocker, resulted in reduced values of all measured electrophysiological parameters irrespective of the phase of hibernation. After application of chloride ion transport inhibitor (bumetanide), the PD in November and December decreased compared with the control incubation by about 80% and 75%, while in January and February by about 40% and 25%, respectively. In January and February dPD increased by four times and three times as compared with November and December. Hibernation reduces net ion flow in isolated frog skin. During the initial period of hibernation the sensitivity of the skin to mechanical stimulation also decreases. Towards the end of hibernation, on the other hand, excitation of mechanosensitive ion channels takes place.
2

Effect of capsaicin and dimethyl sulphoxide (DMSO) on ion transport in isolated skin of frog (Rana esculenta L.)

100%
Kosik-Bogacka D. I., Tyrakowski T.
Folia Biologica
|
2003
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vol. 51
|
issue 1-2
117-123
EN
The effects of capsaicin, dimethyl sulphoxide and pH changes on transport of sodium and/or chlorine ions in an isolated frog skin, were studied using electrophysiological methods, adapted to evaluation of ionic currents occurring in the epithelial tissues and organs. The experiment consisted in measuring potential difference (PD in mV) of an isolated skin of the aquatic frog, Rana esculenta L., placed in a Ussing apparatus. The ionic transport processes were modified through incubation of the tissue in Ringer solution and in Ringer solution supplemented with amiloride, bumetanide, and also with dimethyl sulphoxide. The direct effect of capsaicin and dimethyl sulphoxide (DMSO) on frog skin was assessed while these compounds were added to the Ussing chamber with a pipette and a peristaltic pump. Adaptive reactions of the tissue were assessed following at least 60-min exposure to those compounds. It has been demonstrated that amiloride-inhibited sodium ion transport and acidification of the incubation medium (pH 6.4) inhibited mechanically induced epithelium reactions. Both compounds, capsaicin and DMSO modified ionic transport processes depending on the mechanical stimulation.
3

Transepithelial transport of sodium and chloride ions in isolated skin of the frog, Rana esculenta

100%
Kosik-Bogacka D. I., Tyrakowski T.
|
|
issue 3-4
107-114
EN
Isolated frog skin, mounted in a Ussing apparatus, was investigated electrophysiologically. Application of amiloride, an inhibitor of sodium ion transport, and bumetanide, known to block the transport of chloride ions, revealed the effect of these ions on PD, both under control conditions and following mechanical stimulation. Under control conditions, mechanical stimulation of the skin caused hyperpolarization, i.e. a transient increase in the electrical potential difference. Preincubation in the presence of amiloride, or amiloride plus bumetanide, brought about both a decrease in electrical potential and an inhibition of the reaction upon stimulation. On the other hand, incubation with bumetanide resulted in a decrease in electrical potential, but did not affect the skin reaction after mechanical stimulation. The above results indicate that hyperpolarization of the frog skin following mechanical stimulation is caused by enhanced transepithelial transport of sodium ions which, in turn, is induced by stimulation of sensory receptors.
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