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Comparative in vitro study on the adhesion of probiotic and pathogenic bacteria to different human intestinal cell lines

100%

Lewandowska M., Olejnik A., Neumann M., Krepulec A., Piotrowska J., Tresiak A., Grajek W.

Biotechnologia

2005

issue 2

69-87

The ability of four strains of Lactobacillus sp. two strains of Bifidobacterium sp. and one strain of Listeria mnocytogenes to adhere to human intestinal cell lines Caco-2, HT-29 and Int 407 was examined. Well-developed monolayers of intestinal cells were obtained when initial concentration of Caco-2 cells was 1 x 104/cm2, HT-29 cells 4.2 x 104/cm2, and Int 407 cells 2 x 104/cm2. The appropriate fetal bovine serum additions for Caco-2, HT-29 and Int 407 were 20%, 10% and 10%, respectively. The reduction of serum addition decreased intestinal cell density and prolonged monolayer development. The highest cell densities in epithelial monolayer were obtained in the Int 407 cell cultures. The yield of bacterial adhesion was strain ? dependent. Significant differences were also observed in bacteria adhesion to individual intestinal cell lines. The best adhesion ability to Caco-2 exhibited Lactobacillus rhamnosus GG and Bifidobacterium bifidum. The highest adhesion to HT-29 line demonstrated B. bifidum and Lactobacillus acidophilus LC1. The adhesion of bacteria to Int 407 was much lower. Significant effect on bacteria adhesion has their cell density being in contact with intestinal monolayer. The adherence of Listeria monocytogenes to Caco-2 and HT-29 was very low in the range of 0.2% and 6.0%, respectively.

Adaptations to the air breathing in the posterior intestine of the catfish (Corydoras aeneus, Callichthyidae). A histological and ultrastructural study

75%

Podkowa D., Goniakowska-Witalinska L.

Folia Biologica

2002

vol. 50

issue 1-2

69-82

A light and transmission electron microscopic study of the intestine of catfish C. aeneus shows that the anterior part of the intestine is a site of digestion and absorption and its structure is typical of that of other teleostean fishes. However, in this species the thin- walled posterior intestine is adapted to air breathing. In this region mucosa is smooth and lined with respiratory epithelium with capillary network. Several types of cells are observed in the epithelium: flattened respiratory epithelial cells with short microvili, goblet cells, scarce epithelial cells with numerous longer microvilli, and two types of endocrine cells (EC). The solitary brush cells with several long and thick microvilli described here are the first observation of such cells in the gastrointestinal tract of fishes. Bodies of respiratory epithelial cells lie between capillaries. Their cytoplasm, apart from typical organelles contains dense and lamellar bodies, which are a site of accumulation of surfactant. In regions where capillaries are covered by thin cytoplasmic sheets of respiratory epithelial cells, a thin air-blood barrier is formed, thus enabling gas exchange. Epithelial cells with longer microvilli do not participate in the formation of the air-blood barrier and are probably responsible for absorbtion. EC of the closed type are dispersed within the epithelium. Their cytoplasm contains characteristic round or oval dense core vesicles 69 to 230 nm in diameter. The role of EC and brush cells in the regulation of processes related to absorbtion, and to respiration, is disscused.

The effect of using whole wheat grain in diet of game pheasants on their body weight, dimensions and development of some internal organs

75%

Kokoszynski D., Bernacki Z., Cisowska A.

Folia Biologica

2010

vol. 58

issue 1-2

101-106

The supplementation of game pheasant diet from 5 weeks of age with 30%whole wheat grain instead of feed mixture did not significantly affect their body weight nor most body dimensions. Female and male pheasants fed a diet containing wheat grain had smaller body and trunk lengths, greater chest circumference and greater length of breastbone, and lower thigh and shank size. Female pheasants were characterized by higher indices of compactness and long-leggedness, whereas male pheasants had higher indices of massiveness, compactness and long-leggedness than pheasants fed only feed mixtures. Cock pheasants receiving the wheat diet also had a statistically shorter trunk, however, hens possessed a statistically longer lower thigh. Length of intestine (174.2 cm) and individual parts of the intestine (small intestine 119.9 cm; caeca 43 cm; rectum 11.3 cm) was greater in cocks fed whole wheat grain compared to cocks receiving only feed mixtures (156.1; 107.6; 38.8; 9.7 cm, respectively). An opposite cocks was found in hens. Supplementation of whole wheat grain in the diets significantly (P#0.05) increased liver weight and percentage in females and significantly decreased testicular weight and percentage in males. It was also found that females of both feed-treatment groups had significantly lower weights of heart, liver and spleen, and hens fed only feed mixtures were also characterized by a significantly lower weight of the proventriculus.

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2 Folia Biologica

1 Biotechnologia

1 2010

1 2005

1 2002

1 Bernacki Z.

1 Cisowska A.

1 Goniakowska-Witalinska L.

1 Grajek W.

1 Kokoszynski D.

1 Krepulec A.

1 Lewandowska M.

1 Neumann M.

1 Olejnik A.

1 Piotrowska J.

1 Podkowa D.

1 Tresiak A.

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Comparative in vitro study on the adhesion of probiotic and pathogenic bacteria to different human intestinal cell lines

Adaptations to the air breathing in the posterior intestine of the catfish (Corydoras aeneus, Callichthyidae). A histological and ultrastructural study

The effect of using whole wheat grain in diet of game pheasants on their body weight, dimensions and development of some internal organs