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1

Genetic gain in livestock populations and application of modern biotechniques of gametes and embryons

100%
Szwaczkowski T., Switonski M.
Biotechnologia
|
1993
|
issue 2
46-54
EN
The objective of this paper was to present an influence of some new biotechnologies on the increase of the livestock genetic gain. The effects of the following biotechniques were discussed: embryo transfer, multiple ovulation, in vitro fertilization of oocytes, embryo and semen sexing,cloning as well as creating transgenic animals. Application of these biotechniques increases selection intensity and descreases genetic variability (except for creating transgenic individuals). Moreover, modification of classical models of evaluation of the genetic value of animals is necessary.
2

Haploidy in early bovine embryos produced in vitro

100%
Lechniak D.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 4
363-371
EN
Early bovine embryos (two -to sixteen blastomers) produced in vitro were cytogenetically analysed to determine the incidence of haploidy.Follicular oocytes were matured in vitro and inseminated with sperm prepared using the swim up method.After 2-3 days of culture, chromosome slides were prepared according to the air-drying technique and stained with Giemsa.Altogether 202 embryos produced mathaphase spreads.Of these 4.5% were pure haploid embryos and 5.5% displayed a haploid cell line within mosaic embryos (n/2n and n/3n).The occurence of haploid embryos observed in this study was compared to results of other studies and a possible origin of haploidy was discussed.
3

Methods for obtaining in vitro oocytes and embryos in mammals - possibilities and limitatios

75%
Katska L.
Biotechnologia
|
1995
|
issue 3
33-43
EN
The autors discuss methods through which the reproductive potentialin cattle may be increased.Two procedures for mass production of embryos to be used in cattle breeding schemes are now available, namely superovulation and embryo transfer, nd more recently in vitro embryo production.Hormonal treatmentfor multiple ovulation, nonsurgical embryo collection and embryo transfer are widespread techniques to obtain more offspring from genetivally superor cattle (MOET program).However,the cost can br high and the yield of embryos is unpredictable.
4

Ethical aspects of animal biotechnology

63%
Smorag Z.
Biotechnologia
|
1995
|
issue 3
7-12
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