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1

Cerebellar granular layer degeneration in small cell lung cancer: paraneoplastic cerebellopathy or artifact?

100%
Barcikowska M., Kida E., Joachimowicz E., Siekierzynska A.
Neuropatologia Polska
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1992
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vol. 30
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issue 2
155-163
EN
The aim of our study was to ascertain whether granular cell degeneration represents uniquely an artifiactual or a supravital event in patients with oat cell carcinoma. The material includes 52 cases of small cell lung cancer (SLC). formalin fixed and paraffin embedded representative cerebellar slides were stained routinely (HE, Kluver-Barrera), and some of them served as material for immunohistochemical study. The following antibodies were used? anti-ferritin, anti-GFAP, anti-IgG and anti-C3 complement fraction. Finally 5 cases out of our material could be diagnosed as paraneoplastic cerebellar degeneration (PCD). on the basis of lack of metastases within the CNS and concomitant intensive loss of Purkinje and granule cells. Clinically the cerebellar syndrome was disclosed in 3 cases. In the granular layer prevalence of microglial cell reaction was noted. GFAP-labeled astroglia were not demonstrated in the same intensity. Antisera to the C3 complement fraction showed moderate staining of Purkinje cell cytoplasm and in 4 cases also in granule cell nuclei. The immunopathological changes presently observed and glial cell proliferation could be evidence for a nonartifactual origin of PCD.
2
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A surface antigen delineating a subset of neurons in the primary somatosensory cortex of the mouse

80%
Nowicka D., Liguz-Lecznar M., Skangiel-Kramska J.
Acta Neurobiologiae Experimentalis
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2003
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vol. 63
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issue 3
185-195
EN
Synapsins are a family of proteins associated with synaptic vesicles that are widely used as markers of synaptic terminals. We decided to investigate synapsin I expression in the mouse primary somatosensory cortex (SI). Immunostaining experiments using a polyclonal antibody against C-terminal domain of synapsin Ia/b (anti-SynI-C) showed an unusual pattern in the SI cortex compared to other regions of the neocortex. The staining delineated the cells located in barrel hollows. The immunoreactive product was located on the perikarya and proximal dendrites of gabaergic neurons found in layer IV and VI of the SI cortex. Other anti-synapsin antibodies did not reveal this pattern within the SI cortex, although in the hippocampus all antibodies examined produced a similar pattern of immunostaining. Deglycosylation of sections resulted in complete loss of immunodecoration on the cell perikarya. We suggest that the anti-SynI-C recognizes a saccharide surface epitope, possibly an element of perineuronal nets that is specific for the primary somatosensory cortex.
3

Prognostic significance of p53 protein accumulation in cancer cells obtained from selected group of patients with sporadic colorectal cancer

80%
Paluszkiewicz P., Karski J., Berbe H., Pawlowska-Wakowicz B., Cybulski M., Karski M., Paszkowska A.
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1999
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vol. 40
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issue 2
135-144
EN
p53 gene instability frequently causes accumulation of mutant protein in neoplastic cells. The goal of this study was to evaluate of p53 protein accumulation in tumour cells in relation to colorectal cancer outcome. p53 protein accumulation was tested immunohistochemically using DO-7 and Pab-1620 antibodies. In the group of 80 selected patients with sporadic colorectal cancer, p53 protein accumulation in tumour cells was found significantly more often (52.6% of cases) in cancers localised in the colon, nonmucinous and poorly differentiated. In 5-year follow-up, a shorter survival time was observed in the group of patients with p53 protein accumulation in cancer cells (P<0.05). The differences in p53 protein accumulation found in cancer cells in relation to tumour localisation and their histological type indicate the possibility of p53-independent carcinogenesis in mucinous and right-sided cancers. We indicate the importance of performing the immunohistochemical tests for prediction of the outcome of sporadic colorectal cancers.
4

Malformations of angiogenesis in the low differentiated human carcinomas. Immunohistochemical study

80%
Zoltowska A., Stepinski J., Lewko B., Zamorska B., Roszkiewicz A., Serkies K., Kruszewski W.
Archivum Immunologiae et Therapiae Experimentalis
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2001
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vol. 49
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issue 1
59-62
EN
Our previous observations showed that the perivascular mesenchyma of the thin-walled vessels (capillaries) in cancers may be the source of organ-specific stem cells. We suggested that the cells forming vascular channels in altered stroma participate in the tumor development. This study was designed to examine the distribution of the vessels and their appearance in the breast, lung and colon cancers. Using immunohistochemical methods, we have shown that in the low differentiated tumors both CD31 and factor VIII antigens may be expressed in capillaries chiefly on the periphery of neoplastic foci. Many of these vessels were discontinuous, with interruptions or unformed tubules. Sporadically, CD31 protein and factor VIII antigens were not expressed in capillaries inside the very low differentiated cancer cases. It is difficult to assess by immunohistochemichal means whether the vascular malformations are the primary or secondary phenomena in the malignancy and why these abnormalities were especially visible in some low differentiated cancers.
5

The effect of TCDD dioxin on the rat liver in biochemical and histological assessment

80%
Czepiel J., Biesiada G., Gajda M., Szczepanski W., Szypula K., Dabrowski Z., Mach T.
Folia Biologica
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2010
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vol. 58
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issue 1-2
85-90
EN
Eighteen maleWistar rats were divided into 3 groups of 6 animals each. Two groups received different intraperitoneal doses of TCDD (0.75 and 8Fg) in DMSO solution and the third group (control) received only DMSO on days 0, 7 and 14. On day 21 the animals were sacrificed, and then blood tests, pathological examination and CYP1A1 activity measurement were performed. In rats that received a high dose of dioxin (8 Fg) hepatic lobules revealed parenchymal degeneration and vacuolization of hepatocytes was observed, and also an increased CYP reaction was found in central parts of lobules, around the central vein. The reaction in control and low dose groups was weak. The resorufin level was significantly (P<0.05) higher in the group receiving a low dose of dioxin as compared to the control group. The study confirmed that TCDD damages the rat liver in a dose-dependent manner. Administration of high TCDD doses causing major liver damage also damaged CYP1A1 (based on higher resorufin levels in epiluminescence). TCDD activates CYP1A1, which was confirmed by increased immunohistochemical reactivity of central areas of hepatic lobules.
6
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Nigrostriatal projecting neurons express GDNF receptor subunit RET in adult rats

80%
He S.-M., Zhao Z.-W., Zhao L., Wang X.-L., Jia D., Hou F., Gao G.-D.
Acta Neurobiologiae Experimentalis
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2008
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vol. 68
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issue 3
347-353
EN
Nigrostriatal neurons expressing RET protein, a receptor protein tyrosine kinase of glial cell line-derived neurotrophic factor (GDNF), were investigated in rats using retrograde neural tracing with horseradish peroxidase (HRP) combined with immunohistochemistry. HRP/RET double-labeled neurons were abundantly distributed in the substantia nigra pars compacta ipsilateral to the caudate-putamen stereotaxically injected with HRP. Almost all the HRP-labeled neurons in nigra exhibited RET-like immunoreactivity, which however constituted more than half of the RET-immunoreactive cells. Our results present morphological evidence that GDNF-RET interaction plays important roles in physiological processes of nigrostriatal neuronal circuits of mammals.
7
Content available remote

Dendritic pattern in mouse barrel field after a neonatal vibrissal follicles removal MAP-2 immunohistochemistry

70%
Majewska B., Skangiel-Kramska J.
Acta Neurobiologiae Experimentalis
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1996
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vol. 56
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issue 2
561-566
EN
A distribution of dendrites was studied in mouse barrel field after a neonatal partial lession of vibrissal follicles using anti-MAP-2 immunohistochemistry.The effect of a neonatal vibrissal follicles removal was studied in adult mice: barrels correspodning to intact follicles were enlarged whereas those representing removed follicles had not developed.MAP-2 immunopositive profiles were considered to be dendritic clusters and their packing density (a number per unit area) was calculated in an enlarged barrel and compared to a control barrel in a contralatral hamisphere.A decrease in the packing density of large dendritic clusters, presumably arising from layer V, was observed in an enlarged barrel in comparison to its control counterpart.This result may indicate a selective neonatal lesion of vibrissal follicles.
8

Expression of interleukin-16 by tumor-associated macrophages/activated microglia in high-grade astrocytic brain tumors

70%
Liebrich M., Guo L.-H., Schluesener H. J., Schwab J. M., Dietz K., Will B. E., Meyermann R.
Archivum Immunologiae et Therapiae Experimentalis
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2007
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vol. 55
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issue 1
41-47
EN
Introduction: Macrophages/microglial cells are considered as immune cells in the central nervous system. Interleukin (IL)-16 is a proinflammatory cytokine produced by activated monocytic cells. Materials and Methods: Expression of IL-16 was analyzed by immunohistochemistry in human astrocytic brain tumors and the rat C6 glioblastoma tumor model. IL-16 was detected in both human astrocytic brain tumors and rat C6 glioma. Results: Compared with human control brains, a significant increase in the percentages of parenchymal IL-16+ macrophages/microglia was observed already in grade II astrocytomas, indicating that IL-16+ immunostaining could be a descriptor of a macrophage/microglia subset in astrocytic brain tumors. A further increase was observed at the transition from grade II to III astrocytomas. This increase in IL-16 immunoreactivity correlated with WHO grades of human astrocytic brain tumors. Conclusions: Therefore, IL-16 might be a so far unknown factor in the regulation of the local inflammatory milieu of human and experimental astrocytomas.
9

The site of aromatization in the mouse cryptorchid testis

61%
Kotula-Balak M., Wojciechowska J., Bilinska B.
Folia Biologica
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2001
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vol. 49
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issue 3-4
279-283
EN
Using the mouse cryptorchid model, degenerations of germ cells were observed as well as a reduced size of seminiferous tubules, while the area of the interstitial tissue increased. Aromatase, the enzyme responsible for the conversion of androgens into oestrogens, was immunolocalized in Leydig cells and in germ cells from both scrotal and abdominal testes, and in Sertoli cells only in a control testis. In the cryptorchid testis, aromatase was strongly expressed in a few tubules, including those spermatids that were still present. Other cells inside the tubules were negative for aromatase. In both testes, oestrogen receptors a were expressed only in Leydig cells. Strong aromatase expression in germ cells indicates an additional source of oestrogens in the testis besides the interstitial tissue.
10

Expression of human blood group antigens A and B in kidney and lung of some vertebrates

61%
Tomova E. S., Popov A. A., Sarafian V. S.
Folia Biologica
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2001
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vol. 49
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issue 3-4
251-257
EN
Human blood group antigens (BGA) are genetically determined glycoproteins found in many cells and tissues of different mammals. Their major biological functions are still undefined. There are few investigations analysing the evolutionary aspect of BGA tissue ditribution. The present study is aimed at examining the expression of human A and B antigens in the kidney and lung of some free-living vertebrates. The biotin-streptavidin-peroxidase immunostaining system was applied on kidney and lung paraffin sections derived from free-living representatives of five different vertebrate classes. Excluding the possibility of any non-specific staining by the application of inhibition tests, A and B antigens were demonstrated most constantly in epithelial cells of renal and respiratory tubules. They were also detected in chondrocytes of fish gills, in some muscular and endothelial cells. Single erythrocytes showed a positive cytoplasmic staining only in some higher vertebrates. Human BGA seem to be conserved carbohydrate structures with biological functions probably related to cell integrity and differentiation.
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