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1

Genetic variation of hordein polypeptides in grains of mutants of hulles spring barley (Hordeum vulgare L.) breeding line

100%
Kapala A., Rybinski W.
Journal of Applied Genetics
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1996
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vol. 37
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issue 1
29-35
EN
Hordein polypeptides pattern of 146 mutants in M7 from a hulless spring barley breeding line were examined.The mutants were obtained after treatment of grains of Polish hulless spring barley line, 1N/86, with the chemomutagens N-nirtiso-N-methylurea and sodium azide.Tehmutants were distinguished from the parential line with regard to morphological and yield structure traits.SDS-PAGE of the main hordein fractons, B and C, showed thar the elecrophoretic patterns of the majority of the analysed mutants were similar to the hordein polypeptide spectrum of the parental line.In the previous study, the same mutants showed a significantly better feeding value as compared to hulled cultivars and the rest of the analysed mutants.
2

Genetic control of hordein polypeptides in kernels of spring barley (Hordeum vulgare L.) high-lysine mutant C-67-7

88%
Kapala A., Patyna H.
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vol. 34
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issue 2
133-138
EN
The subject of the studies were F1 and F2 hybrids originating from reciprocal crosses of the highlysine mutant C-67-7 and cultivar Miranda, the forms with different hordein polypeptide spectra. The method of SDS gel electrophoresis was used for an analysis of the main hordein fractions, hordein-1 and hordein-2, from single kernels of barley. It was found that polypeptide spectra of hordein-1 and hordein-2 of the high lysine mutant C-68-7 are determined by codominant alleles at two loci, which are moderately linked.
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