Biology of animal cell in vitro is discussed. Technical conditions for animal cell cultivation are presented. Special attention to two culture systems is given: one is the use of microcarriers for anchorage-dependent cells cultivation, the other concerns the ACUSYST hollow-fiber technology.
Several methods used to cultivate anchorage-dependent animal cells on a large scale are presented. These methods include the cultures on microcarriers in stirred and air-lift bioreactors, the cultures in hollow-fiber and flat-plate membrane bioreactors as well as the cultures attached to porous or fiber solid supports. The critical parameter in anchorage-dependent cultures is the adhesiveness of the cells to the carrier materials and the surface proliferation rate.
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