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1

miR-122 silencing a novel therapy for HCV infections?

100%
Tyczewska A., Bakowska-Zywicka K.
Biotechnologia
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2010
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issue 3
29-35
EN
Hepatitis C (HCV) infection is one of major epidemiological, medical and social concerns in the modern world. In Poland, around 700 000 people have HCV, worldwide the number is as high as 200 million. Current treatment consists of pegylated interferon-alpha and ribavirin, but is limited by the resistance of the viral strains, adverse effects, and high costs. Since HCV infection is a major cause of liver cirrhosis and hepatocellular carcinoma, it is necessary to develop novel antiviral compounds with improved virological response and reduced toxicity. In this article, we describe the results of recent trials to fight the HCV infection using an antagonising miR-122 oligo-LNA probe in primates.
2
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RNA-Seq-based analysis of differential gene expression associated with hepatitis C virus infection in a cell culture

100%
Hojka-Osinska A., Budzko L., Zmienko A., Rybarczyk A., Maillard P., Budkowska A., Figlerowicz M., Jackowiak P.
Acta Biochimica Polonica
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2016
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vol. 63
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issue 4
789-798
EN
Hepatitis C virus (HCV) infection is one of the major causes of chronic liver diseases. Unfortunately, the mechanisms of HCV infection-induced liver injury and host-virus interactions are still not well recognized. To better understand these processes we determined the changes in the host gene expression that occur during HCV infection of Huh-7.5 cells. As a result, we identified genes that may contribute to the immune and metabolic cellular responses to infection. Pathway enrichment analysis indicated that HCV induced an increased expression of genes involved in mitogen-activated protein kinases signaling, adipocytokine signaling, cell cycle and nitrogen metabolism. In addition, the enrichment analyses of processes and molecular functions revealed that the up-regulated genes were mainly implicated in the negative regulation of phosphorylation. Construction of the pathway-gene-process network enabled exploration of a much more complex landscape of molecular interactions. Consequently, several essential processes altered by HCV infection were identified: negative regulation of cell cycle, response to endoplasmic reticulum stress, response to reactive oxygen species, toll-like receptor signaling and pattern recognition receptor signaling. The analyses of genes whose expression was decreased upon HCV infection showed that the latter were engaged in the metabolism of lipids and amino acids. Moreover, we observed disturbance in the cellular antiviral defense. Altogether, our results demonstrated that HCV infection elicits host response that includes a very wide range of cellular mechanisms. Our findings significantly broaden the understanding of complex processes that accompany HCV infection. Consequently, they may be used for developing new host-oriented therapeutic strategies.
3
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Sustained virologic response and IL28B single-nucleotide polymorphisms in patients with chronic hepatitis C treated with pegylated interferon alfa and ribavirin

75%
Jabłonowska E., Piekarska A., Koślińska-Berkan E., Omulecka A., Szymańska B., Wójcik K.
Acta Biochimica Polonica
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2012
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vol. 59
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issue 3
333-337
EN
Introduction. Hepatitis C virus (HCV) infection is a global health problem which can lead to liver cirrhosis or hepatocellular carcinoma in one-fifth of chronically infected patients. Materials and methods. The study group consisted of 123 patients: 90 with HCV mono- and 33 with HIV/HCV co-infection, who were treated with pegylated interferon alfa (Peg-IFN-α) and ribavirin. We analyzed selected pretreatment factors: age, sex, HIV/HCV co-infection, grade of inflammation, necrotic changes and fibrosis in histological analysis of liver bioptates, HCV viral load, HCV genotypes, and single nucleotide polymorphisms (SNPs) of IL28B and tried to find out which of them influence sustained virological response (SVR). The IL28B SNP C/T (rs12979860) was analyzed using Custom® SNP Genotyping Assays (Applied Biosystems). Results. Multivariate analysis demonstrated that after adjusting for the other variables three predictors independently influence SVR, namely genotype 3 of HCV, presence of the CC genotype and age >40 years (OR respectively 15.14, 3.62, and 0.36). HCV mono-infected patients were infected with HCV genotype 3 or 4 less frequently (p=0.0001) compared to HIV/HCV co-infected individuals. In patients with HIV/HCV co-infection the CC variant occurred more frequently whereas CT was found less frequently (p=0.001, p=0.0146, respectively). In patients with HIV/HCV co-infection, 3 and 4 genotype of HCV occurred more frequently compared to patients with HCV mono-infection (p=0.0001). Conclusions. These data suggest that age, HCV genotype and IL28B polymorphism are useful for prediction of the response to treatment with Peg-IFN-α and ribavirin. The more frequent occurrence of HCV genotypes 3 or 4 in patients with HIV/HCV co-infection could be associated with the route of transmission.
4
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Czynniki socjodemograficzne wpływające na poziom wiedzy dotyczącej dróg szerzenia się zakażenia w kontaktach niezwiązanych z procedurami medycznymi wśród pacjentów z WZW typu C

75%
Barański K., Szuba E., Olszanecka-Glinianowicz M., Chudek J.
Annales Academiae Medicae Silesiensis
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2013
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vol. 67
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issue 5
294-302
EN
INTRODUCTION The fear of transmitting viral hepatitis C infection to family members and others can restrict social relations and adversely affect the quality of life related to the social functioning of patients with HCV. AIM The aim of this study was to analyze the level of knowledge of patients with hepatitis C about the ways of HCV transmission as well as to estimate the patient’s quality of life associated with family, social and work functions. MATERIALS AND METHODS Two hundred patients with HCV (108 women) treated in hepatological outpatients clinics in Chorzow and Bytom in 2011 were enrolled in the study. The patients knowledge on the ways of HCV transmission were evaluated using the authors' questionnaire. The quality of life was assessed using questions from the World Health Organization Quality of Life (WHOQOL) questionnaire. RESULTS 80% of men and 70% women have the knowledge that transmission of HCV infection by consuming fluids from the same dish is not possible. 62% of women and 60% of men were aware that HCV is the sexually transmitted infection. More often men than women (58% vs. 51%) did not have knowledge on vertical infection transmission. The level of knowledge on infection transmission was significantly associated with the search for information. 61% of respondents thought that the disease does not limit their social relations, despite the fact that 41% of women and 30% of men often experienced negative feelings, such as anxiety, depression, despair and sadness. 13% of respondents declared that are not able to work, and 17% of women and 11% of men expressed dissatisfaction from their efficiency in everyday life. Social contacts were limited by the lack of knowledge that transmission of HCV of infection by the use of the same dishes is not possible (44.9% vs. 35.5%, p = 0.08). CONCLUSIONS 1. The low level of knowledge among patients with hepatitis C concerning HCV infection transmission may limit social contacts and impair family functioning as well as decrease their quality of life. 2. The self-search of information about the disease is related to education level. The increase in knowledge level in the population of patients with hepatitis C requires health education system development in this aspect.
PL
WPROWADZENIE Lęk przed zakażeniem się wirusowym zapaleniem wątroby (WZW) typu C przez członków rodziny i inne osoby z otoczenia ogranicza kontakty społeczne i wpływa niekorzystnie na jakość życia chorych. CEL PRACY Celem niniejszej pracy była ocena jakości życia związanej z funkcjonowaniem społecznym oraz stanu wiedzy pacjentów z WZW typu C na temat dróg szerzenia się zakażenia. MATERIAŁ I METODA Badaniem objęto 200 dorosłych pacjentów (w tym 108 kobiet) z WZW typu C leczonych w poradniach hepatologicznych w Chorzowie oraz w Bytomiu w 2011 r. Wiedzę chorych na temat dróg szerzenia się zakażenia oceniano na podstawie autorskiej ankiety, natomiast do oceny jakości życia wykorzystano pytania z kwestionariusza Światowej Organizacji Zdrowia – Jakość Życia (WHOQOL). WYNIKI Wiedzę o braku możliwości przeniesienia zakażenia WZW typu C poprzez spożywanie płynów z tego samego naczynia posiadało 80% mężczyzn i 70% kobiet; 62% kobiet i 60% mężczyzn wiedziało o ryzyku zakażenia związanym ze stosunkiem seksualnym. Mężczyźni częściej niż kobiety (58% vs. 51%) nie wiedzieli, że zakażenie może nastąpić drogą wertykalną. Poziom wiedzy w znacznym stopniu był związany z jej poszukiwaniem. 61% badanych uważało, że choroba nie ogranicza ich kontaktów społecznych; 41% kobiet i 30% mężczyzn często doświadczało negatywnych odczuć, takich jak lęk, depresja, rozpacz i przygnębienie; 13% respondentów obu płci zadeklarowało, że wcale nie są gotowi do podjęcia pracy, a brak zadowolenia ze swojej wydolności w życiu codziennym wyraziło odpowiednio 17% kobiet i 11% mężczyzn. Brak wiedzy o tym, że zakażenie nie szerzy się poprzez stosowanie wspólnych naczyń, ograniczał kontakty społeczne (44,9% vs. 35,5%, p = 0,08). WNIOSKI 1. Niski poziom wiedzy chorych z WZW typu C na temat dróg szerzenia się zarażenia może ograniczać ich kontakty społeczne i upośledzać funkcjonowania w rodzinie, a przez to obniżać jakość ich życia. 2. Samodzielne poszukiwanie wiedzy na temat choroby jest uwarunkowane poziomem wykształcenia. Zwiększenie wiedzy w populacji pacjentów chorujących na WZW typu C wymaga poszerzenia systemu edukacji zdrowotnej.
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The Comparison of Sharps Injuries Reported by Doctors Versus Nurses from Surgical Wards in the Context of the Prevalence of HBV, HCV and HIV Infections

63%
Gańczak M., Bohatyrewicz A., Korzeń M., Karakiewicz B.
Polish Journal of Surgery
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2012
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vol. 84
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issue 4
190-195
EN
The aim of the study was to evaluate the nature and frequency of sharps injuries among doctors and nurses from the same surgical/gynecological wards and the prevalence of HBV/HCV/HIV infection.Material and methods. An anonymous cross-sectional sero-survey, with ELISA system used to detect anti-HBc, anti-HCV, anti-HIV, was conducted among 89 doctors and 414 nurses from 16 randomly selected hospitals in West Pomerania, Poland, between January-June 2009.Results. During the preceding 12 months, 82% doctors and 44.4% nurses (p<0.0001) had sustained at least one sharps injury; 12.3% doctors vs 2.2% nurses (p<0.003) sustained more than 10 injuries. The multivariable regression model revealed that being a doctor was associated with a greater odds (OR 4.2) of being injured with sharps. Sixty nine percent of nurses sustained a hollow-bore needle injury vs 8.9% doctors; p<0.001. Anti-HBc were found in 16.4% of doctors and 11.2% of nurses, p>0.28; anti-HCV - in 1.1% of doctors vs 1.4% of nurses, p>0.79; no anti-HIV positive cases were found. The analysis of potential risk factors for contracting a HBV revealed that for both job categories only length of employment was associated with an increased odds of being infected.Conclusions. Although the prevalence of HBV/HCV infection between doctors and nurses does not differ significantly, modifiable risk factors for contracting a BBI such as frequency and nature of sharps injuries may differ, which call for tailoring preventive measures to specific job categories. Long lasting exposure to injury events should be taken into consideration while assessing the risk for accuiring an occupational infection with HBV, HCV or HIV.
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