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1

Utillity of starch raw materials for biosynthesis of glucose oxidase by Aspegillus niger

100%
Fiedurek J., Szczodrak J.
Biotechnologia
|
1994
|
issue 3
37-44
EN
Potato starch and its hydrolizates were used to substitute glucose cabon souces to synthesize glucose oxidase (GOD) by free and immobiized on pumice stones A.niger mycellium.The basic conditions for starch hydrolisis, securing the maximal enzyme yields, were optimized.The immobilized A.niger mycelium was able to produce the enzyme at a small loss of its acting (about 28%) during ten 24-h batches.Free mycelium produced about 1.5 times higher GOD activity than the immobilized mycelium on glucose and starch hydrolizates.However,the latter was more stable, reaching faster the maximum of enzyme productivity, and it could be used many times with almost the same efficiency.Thus,the total enzyme yields produced by immunobilized mycelium were much higher than those obtained using the free one.
2

Utility of unconventional oxygenation of culture in gluconic acid fermentation

100%
Fiedurek J., Pielecki J., Gromada A.
Biotechnologia
|
1999
|
issue 4
179-185
EN
A new apparatus for controlling dissolved oxygen concentration in the culture media was tested. Using the apparatus, the automatic dosing of hydrogen peroxide (decomposed by catalase to oxygen and water) was possible, and a significant increase of dissolved oxygen, gluconic acid (3-fold) and over 5-fold intracellular catalase activity were obtained in comparison with the traditional aeration. It also minimises contamination, lowers the expenses on power consumption of aeration, reduces foaming and, consequently, high cost of antifoam emulsion and bacteriological filters.
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