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1

On-line monitoring of A. niger metabolic activity in biotransformation of glucose to gluconic acid

100%
Podgorski W.
Biotechnologia
|
2004
|
issue 1
169-186
EN
Control parameters directly connected with metabolism of microorganism that reflect its actual activity in gluconic acid biosynthesis can be chosen basing on mass balance of the process. The relatively high correlation between parameters, normally determined via sample analysis and microorganisms? respiratory activity, enabled on-line estimation of biomass growth, product formation, and substrate uptake rate. The estimated parameters were continuously measured and employed to on-line control of gluconic acid overproduction by Aspergillus niger W78B strain. The application of the noninvasive technique for parameters determinations allowed obtaining high effectiveness of product formation characterized by the yield coefficient that equals 1.0 g g-1 and product formation rate amounted to 5 g dm-3 h-1. The estimation of the parameters cannot be used instead of their quantitative determinations, but it gives a possibility of on-line monitoring and control of the process.
2

Utility of unconventional oxygenation of culture in gluconic acid fermentation

88%
Fiedurek J., Pielecki J., Gromada A.
Biotechnologia
|
1999
|
issue 4
179-185
EN
A new apparatus for controlling dissolved oxygen concentration in the culture media was tested. Using the apparatus, the automatic dosing of hydrogen peroxide (decomposed by catalase to oxygen and water) was possible, and a significant increase of dissolved oxygen, gluconic acid (3-fold) and over 5-fold intracellular catalase activity were obtained in comparison with the traditional aeration. It also minimises contamination, lowers the expenses on power consumption of aeration, reduces foaming and, consequently, high cost of antifoam emulsion and bacteriological filters.
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