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From neoplastic neural development to gene therapy of brain tumors ? IGF-I antisense and triple helix approaches

100%

Trojan J.

Biotechnologia

2003

issue 2

280-289

IGF-I, insulin ? like growth factor I, seems to play a major role in the normal and tumoral development of the nervous system. Glioblastoma is the most frequent brain tumor in man and is usually fatal. Both human and rat glioma cells express high amounts of IGF-I. When rat glioma cells are transfected with vectors expressing either IGF-I antisense RNA or inducing IGF RNA ? DNA triple helix, the synthesis of IGF-I was stopped on translation or transcription levels, respectively. Down-regulation in the expression of IGF-I coincides with the reappearance of B-7 and MHC class I antigens at the surface of transfected cells. When injected subcutaneously, the transfected cancer cells initiate an immune reaction involving CD8+ lymphocytes, followed by tumor regression. The ?anti-gene? strategy for clinical therapy of glioblastoma, and other tumors expressing IGF-I such hepatomas were introduced in University Hospitals of Cleveland (USA), Shanghai ( China) , Krakow and Bydgoszcz (Poland).

Gliomas: association of histology and molecular genetic analysis of chromosomes 1p, 10q, and 19q

86%

Gresner S. M., Rieske P., Wozniak K., Piaskowski S., Jaskolski D. J., Skowronski W., Sikorska B., Papierz W., Liberski P. P.

Acta Neurobiologiae Experimentalis

2007

vol. 67

issue 2

103-112

The aim of our study was to evaluate the frequencies of loss of heterozygosity (LOH) on chromosomes 1p, 10q, and 19q in gliomas and to correlate them with the histological diagnosis and with patient age and gender. We found deletions within chromosome 1p to be significantly associated with the histological subtype of glial tumor (P < 0.05); frequency of 1p deletions increased from astrocytoma (0%) through glioblastoma (31%) and oligoastrocytoma (57%) to oligodendroglioma (63%). In patients with 1p LOH, the odds for having astrocytoma or glioblastoma were approximately 10-fold and 4-fold lower, respectively, than oligodendroglioma. The odds for having oligoastrocytoma were similar to oligodendroglioma (OR = 1.3). The frequency of 10q LOH in patients with glioblastoma was significantly higher than in patients with oligodendroglioma (89% vs. 36%; P < 0.005). In patients with oligodendroglioma, most cases with LOH on chromosome 1p also had LOH 19q (90%), one case of 1p LOH also had a deletion on 10q. Statistical analyses revealed a significant association between deletions on 1p and 19q (P < 0.05). Our data provide evidence that use of molecular genetic analyses of chromosomes 1p, 19q, and 10q might improve the diagnosis of gliomas.

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1 Acta Neurobiologiae Experimentalis

1 Biotechnologia

1 2007

1 2003

1 Gresner S. M.

1 Jaskolski D. J.

1 Liberski P. P.

1 Papierz W.

1 Piaskowski S.

1 Rieske P.

1 Sikorska B.

1 Skowronski W.

1 Trojan J.

1 Wozniak K.

Search results

From neoplastic neural development to gene therapy of brain tumors ? IGF-I antisense and triple helix approaches

Gliomas: association of histology and molecular genetic analysis of chromosomes 1p, 10q, and 19q