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  1. 2 Biotechnologia

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  1. 1 2003

  2. 1 1999

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  1. 2 Mikula A.

  2. 2 Rybczynski J. J.

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1

Present status of Gentiana taxa biotechnology

100%
Mikula A., Rybczynski J. J.
Biotechnologia
|
1999
|
issue 4
76-91
EN
Data on the tissue culture and genetic manipulation of several species of the genus Gentiana in support on the review of literature are presented.
2

Cryopreservation of suspended cells of selected Gentiana

100%
Mikula A., Rybczynski J. J.
|
2003
|
issue 3
39-51
EN
Cryopreservation is recognised as the most efficient method of plant genome preservation and embryogenic potential of plant in vitro cultures with non-changed status. Because of the risk of biological degradation of tissue during freezing treatment and it various sensibility on the chilling stress, the individual treatment with cryoprotectance and parameters of the temperature decreasing is highly required. In this presentation, following subjects will be discussed: 1) description of suspension cultures of various gentians, 2) cell pretreatments for surviving the stress of low temperature, 3) rearrangement of cell cytoplasm because of high sucrose concentrations, 4) the role of sucrose in the development of freezing tolerance and surviving of gentaiana suspensions, 5) the effect of strong plasmolysis induced by 3 M glucose.
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